PMID- 33531052
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1478-6362 (Electronic)
IS  - 1478-6354 (Print)
IS  - 1478-6354 (Linking)
VI  - 23
IP  - 1
DP  - 2021 Feb 2
TI  - Silk fibroin hydrogel scaffolds incorporated with chitosan nanoparticles repair 
      articular cartilage defects by regulating TGF-beta1 and BMP-2.
PG  - 50
LID - 10.1186/s13075-020-02382-x [doi]
LID - 50
AB  - Cartilage defects frequently occur around the knee joint yet cartilage has 
      limited self-repair abilities. Hydrogel scaffolds have excellent potential for 
      use in tissue engineering. Therefore, the aim of the present study was to assess 
      the ability of silk fibroin (SF) hydrogel scaffolds incorporated with chitosan 
      (CS) nanoparticles (NPs) to repair knee joint cartilage defects. In the present 
      study, composite systems of CS NPs incorporated with transforming growth 
      factor-beta1 (TGF-beta1; TGF-beta1@CS) and SF incorporated with bone morphogenetic 
      protein-2 (BMP-2; TGF-beta1@CS/BMP-2@SF) were developed and characterized with 
      respect to their size distribution, zeta potential, morphology, and release of 
      TGF-beta1 and BMP-2. Bone marrow stromal cells (BMSCs) were co-cultured with 
      TGF-beta1@CS/BMP-2@SF extracts to assess chondrogenesis in vitro using a cell 
      counting kit-8 assay, which was followed by in vivo evaluations in a rabbit model 
      of knee joint cartilage defects. The constructed TGF-beta1@CS/BMP-2@SF composite 
      system was successfully characterized and showed favorable biocompatibility. In 
      the presence of TGF-beta1@CS/BMP-2@SF extracts, BMSCs exhibited normal cell 
      morphology and enhanced chondrogenic ability both in vitro and in vivo, as 
      evidenced by the promotion of cell viability and the alleviation of cartilage 
      defects. Thus, the TGF-beta1@CS/BMP-2@SF hydrogel developed in the present study 
      promoted chondrogenic ability of BMSCs both in vivo and in vitro by releasing 
      TGF-beta1 and BMP-2, thereby offering a novel therapeutic strategy for repairing 
      articular cartilage defects in knee joints.
FAU - Li, Yuan
AU  - Li Y
AD  - Department of Joint Surgery, Linyi People's Hospital, Linyi, 276000, People's 
      Republic of China.
FAU - Liu, Yanping
AU  - Liu Y
AD  - Department of Orthopaedics of Integrated traditional and Western Medicine, Linyi 
      People's Hospital, Linyi, 276000, People's Republic of China.
FAU - Guo, Qiang
AU  - Guo Q
AD  - Department of Hand and Foot Surgery, Linyi People's Hospital, Linyi, 276000, 
      People's Republic of China. GQ13573979283@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210202
PL  - England
TA  - Arthritis Res Ther
JT  - Arthritis research & therapy
JID - 101154438
RN  - 0 (Hydrogels)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 9007-76-5 (Fibroins)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - *Cartilage, Articular
MH  - Cell Differentiation
MH  - *Chitosan
MH  - Chondrogenesis
MH  - *Fibroins
MH  - Hydrogels
MH  - *Nanoparticles
MH  - Rabbits
MH  - Tissue Engineering
MH  - Tissue Scaffolds
MH  - Transforming Growth Factor beta1
PMC - PMC7856775
OTO - NOTNLM
OT  - Articular cartilage defects
OT  - Bone morphogenetic protein-2
OT  - Hydrogel
OT  - Tissue-engineered cartilage
OT  - Transforming growth factor-beta1
COIS- The authors declare that they have no competing interests.
EDAT- 2021/02/04 06:00
MHDA- 2021/06/22 06:00
PMCR- 2021/02/02
CRDT- 2021/02/03 05:37
PHST- 2020/03/05 00:00 [received]
PHST- 2020/12/07 00:00 [accepted]
PHST- 2021/02/03 05:37 [entrez]
PHST- 2021/02/04 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/02/02 00:00 [pmc-release]
AID - 10.1186/s13075-020-02382-x [pii]
AID - 2382 [pii]
AID - 10.1186/s13075-020-02382-x [doi]
PST - epublish
SO  - Arthritis Res Ther. 2021 Feb 2;23(1):50. doi: 10.1186/s13075-020-02382-x.