PMID- 33531194
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210414
IS  - 0975-9476 (Print)
IS  - 0976-2809 (Electronic)
IS  - 0975-9476 (Linking)
VI  - 12
IP  - 1
DP  - 2021 Jan-Mar
TI  - Elicited soybean extract attenuates proinflammatory cytokines expression by 
      modulating TLR3/TLR4 activation in high-fat, high-fructose diet mice.
PG  - 43-51
LID - S0975-9476(21)00004-8 [pii]
LID - 10.1016/j.jaim.2021.01.003 [doi]
AB  - BACKGROUND: The high-fat, high-fructose diet (HFFD) provokes overnutrition and 
      inflammation directly, mainly through Toll-like receptors (TLRs). Soybean 
      (Glycine max L.) contains isoflavone that can be transformed into glyceollin by 
      microbial and physical stimuli. Glyceollin possesses many beneficial effects on 
      health. OBJECTIVE: This study evaluates the beneficial effect of soybean extract 
      elicited by Saccharomyces cerevisiae and light (ESE) on dendritic cells (DCs) 
      profile and naive T cells in HFFD mice. MATERIALS AND METHODS: Female Balb/C mice 
      were fed with HFFD for 24 weeks then orally administered with simvastatin 
      2.8 mg/kg BW or ESE 78, 104, and 130 mg/kg BW at the last four weeks. The 
      expression of splenic CD11c(+)TLR3(+), CD11c(+)TLR4(+), NFkappaB(+), 
      CD11c(+)IL-17(+), CD11c(+)TNF-alpha(+), CD4(+)CD62L(+), and CD8(+)CD62L(+) subsets 
      was measured by flow cytometry. The molecular docking has been measured using 
      Pyrx 0.8, displayed in PyMol and Biovia Discovery Studio. RESULT: HFFD 
      significantly increased CD11c(+)TLR3(+), CD11c(+)TLR4(+), NFkappaB(+), 
      CD11c(+)IL-17(+), CD11c(+)TNF-alpha(+) expression and decreased CD4(+)CD62L(+) and 
      CD8(+)CD62L(+) (p < 0.05) compared to normal diet (ND) groups. ESE reduced 
      CD11c(+)TLR3(+), CD11c(+)TLR4(+), thereby decreasing NFkappaB(+), as well as 
      decreased the CD11c(+)IL-17(+), CD11c(+)TNF-alpha(+), and restores CD4(+)CD62L(+) and 
      CD8(+)CD62L(+) subsets in HFFD mice. Glyceollin II exhibited the best binding 
      affinity with an average energy of -7.3 kcal/mol to TLR3 and -7.9 kcal/mol to 
      TLR4. CONCLUSION: The bioactive compound in ESE act synergistically to modulate 
      TLR3/TLR4 activation, reduced NFkappaB, IL-17, and TNF-alpha, and restores naive T cells 
      expression in HFFD mice. ESE was a favorable candidate to mitigate chronic 
      inflammation.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Atho'illah, Mochammad Fitri
AU  - Atho'illah MF
AD  - Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya 
      University, 65145, Malang, East Java, Indonesia.
FAU - Safitri, Yunita Diyah
AU  - Safitri YD
AD  - Medical Laboratory Technology Program, Sekolah Tinggi Ilmu Kesehatan Karya Putra 
      Bangsa, 66291, Tulungagung, East Java, Indonesia.
FAU - Nur'aini, Farida Dewi
AU  - Nur'aini FD
AD  - Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya 
      University, 65145, Malang, East Java, Indonesia.
FAU - Widyarti, Sri
AU  - Widyarti S
AD  - Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya 
      University, 65145, Malang, East Java, Indonesia.
FAU - Tsuboi, Hideo
AU  - Tsuboi H
AD  - Department of Immunology, Nagoya University Graduate School of Medicine, 65 
      Tsurumai-cho, Showaku, Nagoya, 466-8550, Japan.
FAU - Rifa'i, Muhaimin
AU  - Rifa'i M
AD  - Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya 
      University, 65145, Malang, East Java, Indonesia; Center of Biosystem Study, LPPM 
      of Brawijaya University, 65145, Malang, East Java, Indonesia. Electronic address: 
      rifa123@ub.ac.id.
LA  - eng
PT  - Journal Article
DEP - 20210130
PL  - United States
TA  - J Ayurveda Integr Med
JT  - Journal of Ayurveda and integrative medicine
JID - 101551404
PMC - PMC8039419
OTO - NOTNLM
OT  - Glyceollin
OT  - High-fat
OT  - High-fructose
OT  - IL-17
OT  - TLR3/TLR4
OT  - TNF-alpha
EDAT- 2021/02/04 06:00
MHDA- 2021/02/04 06:01
PMCR- 2021/01/30
CRDT- 2021/02/03 05:39
PHST- 2020/11/11 00:00 [received]
PHST- 2020/12/31 00:00 [revised]
PHST- 2021/01/08 00:00 [accepted]
PHST- 2021/02/04 06:00 [pubmed]
PHST- 2021/02/04 06:01 [medline]
PHST- 2021/02/03 05:39 [entrez]
PHST- 2021/01/30 00:00 [pmc-release]
AID - S0975-9476(21)00004-8 [pii]
AID - 10.1016/j.jaim.2021.01.003 [doi]
PST - ppublish
SO  - J Ayurveda Integr Med. 2021 Jan-Mar;12(1):43-51. doi: 10.1016/j.jaim.2021.01.003. 
      Epub 2021 Jan 30.