PMID- 33531564
OWN - NLM
STAT- MEDLINE
DCOM- 20211115
LR  - 20211115
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Feb 2
TI  - Alterations in erythrocyte membrane transporter expression levels in type 2 
      diabetic patients.
PG  - 2765
LID - 10.1038/s41598-021-82417-8 [doi]
LID - 2765
AB  - Type 2 diabetes mellitus (T2DM) is one of the most common multifactorial diseases 
      and several membrane transporters are involved in its development, complications 
      and treatment. We have recently developed a flow-cytometry assay panel for the 
      quantitative determination of red cell membrane protein levels with potential 
      relevance in diseases. Here we report a detailed phenotypic analysis of a medium 
      scale, clinically based study on the expression of T2DM-related membrane 
      proteins, the GLUT1, GLUT3, MCT1, URAT1, ABCA1, ABCG2 and the PMCA4 transporters 
      in erythrocytes. By comparing age-matched control subjects and three groups of 
      T2DM patients (recently diagnosed, successfully managed, and patients with 
      disease-related complications), we found significant differences in the membrane 
      expression levels of the transporters in these groups. This is a first detailed 
      analysis of T2DM related alterations in erythrocyte membrane transporter protein 
      levels, and the results suggest significant changes in some of the transporter 
      expression levels in various patient groups. By performing a further, more 
      detailed analysis of the clinical and molecular biology parameters, these data 
      may serve as a basis of establishing new, personalized diagnostic markers helping 
      the prevention and treatment of type 2 diabetes.
FAU - Szabo, Edit
AU  - Szabo E
AD  - Institute of Enzymology, ELKH Research Centre for Natural Sciences, Center of 
      Excellence By Hungarian Academy of Sciences, Magyar tudosok korutja 2, 1117, 
      Budapest, Hungary.
FAU - Kulin, Anna
AU  - Kulin A
AD  - Institute of Enzymology, ELKH Research Centre for Natural Sciences, Center of 
      Excellence By Hungarian Academy of Sciences, Magyar tudosok korutja 2, 1117, 
      Budapest, Hungary.
FAU - Koranyi, Laszlo
AU  - Koranyi L
AD  - Drug Research Center, Balatonfured, Hungary.
FAU - Literati-Nagy, Botond
AU  - Literati-Nagy B
AD  - Drug Research Center, Balatonfured, Hungary.
FAU - Cserepes, Judit
AU  - Cserepes J
AD  - CellPharma Kft, Budapest, Hungary.
FAU - Somogyi, Aniko
AU  - Somogyi A
AD  - 2nd Department of Internal Medicine, Semmelweis University, Budapest, Hungary.
FAU - Sarkadi, Balazs
AU  - Sarkadi B
AD  - Institute of Enzymology, ELKH Research Centre for Natural Sciences, Center of 
      Excellence By Hungarian Academy of Sciences, Magyar tudosok korutja 2, 1117, 
      Budapest, Hungary.
AD  - Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, 
      Hungary.
FAU - Varady, Gyorgy
AU  - Varady G
AD  - Institute of Enzymology, ELKH Research Centre for Natural Sciences, Center of 
      Excellence By Hungarian Academy of Sciences, Magyar tudosok korutja 2, 1117, 
      Budapest, Hungary. varady.gyorgy@ttk.hu.
LA  - eng
PT  - Journal Article
PT  - Observational Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20210202
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Membrane Transport Proteins)
SB  - IM
MH  - Aged
MH  - Biomarkers/blood/metabolism
MH  - Blood Glucose/*metabolism
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/blood/*diagnosis/metabolism
MH  - Erythrocyte Membrane/*metabolism
MH  - Erythrocytes/cytology/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Membrane Transport Proteins/*blood/metabolism
MH  - Middle Aged
PMC - PMC7854743
COIS- The authors declare no competing interests.
EDAT- 2021/02/04 06:00
MHDA- 2021/11/16 06:00
PMCR- 2021/02/02
CRDT- 2021/02/03 05:53
PHST- 2020/06/15 00:00 [received]
PHST- 2021/01/18 00:00 [accepted]
PHST- 2021/02/03 05:53 [entrez]
PHST- 2021/02/04 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
PHST- 2021/02/02 00:00 [pmc-release]
AID - 10.1038/s41598-021-82417-8 [pii]
AID - 82417 [pii]
AID - 10.1038/s41598-021-82417-8 [doi]
PST - epublish
SO  - Sci Rep. 2021 Feb 2;11(1):2765. doi: 10.1038/s41598-021-82417-8.