PMID- 33531650
OWN - NLM
STAT- MEDLINE
DCOM- 20211115
LR  - 20211115
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Feb 2
TI  - Alterations in the gut bacterial microbiome in people with type 2 diabetes 
      mellitus and diabetic retinopathy.
PG  - 2738
LID - 10.1038/s41598-021-82538-0 [doi]
LID - 2738
AB  - Gut bacterial microbiome dysbiosis in type 2 Diabetes Mellitus (T2DM) has been 
      reported, but such an association with Diabetic Retinopathy (DR) is not known. We 
      explored possible link between gut bacterial microbiome dysbiosis and DR. Using 
      fecal samples of healthy controls (HC) and people with T2DM with/without DR, gut 
      bacterial communities were analysed using 16S rRNA gene sequencing and data 
      analysed using QIIME and R software. Dysbiosis in the gut microbiomes, at phyla 
      and genera level, was observed in people with T2DM and DR compared to HC. People 
      with DR exhibited greater discrimination from HC. Microbiomes of people with T2DM 
      and DR were also significantly different. Both DM and DR microbiomes showed a 
      decrease in anti-inflammatory, probiotic and other bacteria that could be 
      pathogenic, compared to HC, and the observed change was more pronounced in people 
      with DR. This is the first report demonstrating dysbiosis in the gut microbiome 
      (alteration in the diversity and abundance at the phyla and genera level) in 
      people with DR compared to HC. Such studies would help in developing novel and 
      targeted therapies to improve treatment of DR.
FAU - Das, Taraprasad
AU  - Das T
AD  - Smt. Kanuri Santhamma Centre for Vitreo-Retinal Diseases, L. V. Prasad Eye 
      Institute, Kallam Anji Reddy Campus, Hyderabad, 500034, India.
FAU - Jayasudha, Rajagopalaboopathi
AU  - Jayasudha R
AD  - Prof. Brien Holden Eye Research Centre, L. V. Prasad Eye Institute, Kallam Anji 
      Reddy Campus, Hyderabad, 500034, India.
FAU - Chakravarthy, SamaKalyana
AU  - Chakravarthy S
AD  - Prof. Brien Holden Eye Research Centre, L. V. Prasad Eye Institute, Kallam Anji 
      Reddy Campus, Hyderabad, 500034, India.
FAU - Prashanthi, Gumpili Sai
AU  - Prashanthi GS
AD  - Prof. Brien Holden Eye Research Centre, L. V. Prasad Eye Institute, Kallam Anji 
      Reddy Campus, Hyderabad, 500034, India.
FAU - Bhargava, Archana
AU  - Bhargava A
AD  - Internal Medicine, L. V. Prasad Eye Institute, Kallam Anji Reddy Campus, 
      Hyderabad, 500034, India.
FAU - Tyagi, Mudit
AU  - Tyagi M
AD  - Smt. Kanuri Santhamma Centre for Vitreo-Retinal Diseases, L. V. Prasad Eye 
      Institute, Kallam Anji Reddy Campus, Hyderabad, 500034, India.
FAU - Rani, Padmaja Kumari
AU  - Rani PK
AD  - Smt. Kanuri Santhamma Centre for Vitreo-Retinal Diseases, L. V. Prasad Eye 
      Institute, Kallam Anji Reddy Campus, Hyderabad, 500034, India.
FAU - Pappuru, Rajeev Reddy
AU  - Pappuru RR
AD  - Smt. Kanuri Santhamma Centre for Vitreo-Retinal Diseases, L. V. Prasad Eye 
      Institute, Kallam Anji Reddy Campus, Hyderabad, 500034, India.
FAU - Sharma, Savitri
AU  - Sharma S
AD  - Prof. Brien Holden Eye Research Centre, L. V. Prasad Eye Institute, Kallam Anji 
      Reddy Campus, Hyderabad, 500034, India.
FAU - Shivaji, Sisinthy
AU  - Shivaji S
AD  - Prof. Brien Holden Eye Research Centre, L. V. Prasad Eye Institute, Kallam Anji 
      Reddy Campus, Hyderabad, 500034, India. shivas@lvpei.org.
LA  - eng
GR  - EMR/2017/000815; August 2018/Department of Science and Technology, Ministry of 
      Science and Technology, India/
PT  - Journal Article
PT  - Observational Study
DEP - 20210202
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Case-Control Studies
MH  - DNA, Bacterial
MH  - Diabetes Mellitus, Type 2/*complications/immunology/microbiology
MH  - Diabetic Retinopathy/*immunology/microbiology
MH  - Dysbiosis/complications/*diagnosis/immunology/microbiology
MH  - Feces/microbiology
MH  - Female
MH  - Gastrointestinal Microbiome/*immunology
MH  - Healthy Volunteers
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - RNA, Ribosomal, 16S/genetics
PMC - PMC7854632
COIS- The authors declare no competing interests.
EDAT- 2021/02/04 06:00
MHDA- 2021/11/16 06:00
PMCR- 2021/02/02
CRDT- 2021/02/03 05:54
PHST- 2020/03/25 00:00 [received]
PHST- 2021/01/21 00:00 [accepted]
PHST- 2021/02/03 05:54 [entrez]
PHST- 2021/02/04 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
PHST- 2021/02/02 00:00 [pmc-release]
AID - 10.1038/s41598-021-82538-0 [pii]
AID - 82538 [pii]
AID - 10.1038/s41598-021-82538-0 [doi]
PST - epublish
SO  - Sci Rep. 2021 Feb 2;11(1):2738. doi: 10.1038/s41598-021-82538-0.