PMID- 33532367
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210206
IS  - 2239-7132 (Print)
IS  - 2239-7132 (Electronic)
IS  - 2239-7132 (Linking)
VI  - 9
IP  - 4
DP  - 2020 Dec 3
TI  - Identification and evaluation of antimicrobial resistance of enterococci isolated 
      from raw ewes' and cows' milk collected in western Sicily: a preliminary 
      investigation.
PG  - 8406
LID - 10.4081/ijfs.2020.8406 [doi]
LID - 8406
AB  - The present work was carried out to investigate the Antimicrobial Resistance 
      (AMR) of enterococci isolated from raw ewes' and cows' milk. The samples were 
      collected from eighteen semi-extensive dairy sheep and cow farms throughout 
      western Sicily. Plate counts, carried out on Rapid Enterococcus Agar commonly 
      used to detect food enterococci, revealed a maximal enterococcal concentration of 
      approximately 4.58 Log Colony Forming Unit (CFU)/mL. Colonies were isolated and 
      differentiated based on genetic analysis by Randomly Amplified Polymorphic DNA 
      (RAPD)-PCR. Thirty-eight different strains were identified. Analysis by a 
      species-specific multiplex PCR assay grouped the strains into three Enterococcus 
      species such as Enterococcus durans, Enterococcus faecalis and Enterococcus 
      faecium. The 38 strains were also investigated for their antimicrobial resistance 
      by a phenotypic approach. All 38 Enterococcus displayed resistance to at least 
      one or more of the antimicrobials tested confirmed that the dairy enterococci 
      could be a vector for the dissemination of antimicrobial resistance. This work 
      showed that enterococci with AMR traits are commonly present in semiextensive 
      dairy sheep and cow farms of western Sicily pointed out the relevance of 
      informing dairy makers and veterinary regarding the antimicrobial use in order to 
      mitigate problems of public health and veterinary medicine.
CI  - (c)Copyright: the Author(s).
FAU - Palmeri, Marisa
AU  - Palmeri M
AD  - Institute for Experimental Veterinary Medicine of Sicily A. Mirri, Palermo.
FAU - Mancuso, Isabella
AU  - Mancuso I
AD  - Institute for Experimental Veterinary Medicine of Sicily A. Mirri, Palermo.
FAU - Gaglio, Raimondo
AU  - Gaglio R
AD  - Department of Agricultural, Food and Forest Sciences, University of Palermo, 
      Palermo, Italy.
FAU - Arcuri, Luigi
AU  - Arcuri L
AD  - Local Health Unit, Palermo.
FAU - Barreca, Santino
AU  - Barreca S
AD  - Institute for Experimental Veterinary Medicine of Sicily A. Mirri, Palermo.
FAU - Barbaccia, Pietro
AU  - Barbaccia P
AD  - Department of Agricultural, Food and Forest Sciences, University of Palermo, 
      Palermo, Italy.
FAU - Scatassa, Maria Luisa
AU  - Scatassa ML
AD  - Institute for Experimental Veterinary Medicine of Sicily A. Mirri, Palermo.
LA  - eng
PT  - Journal Article
DEP - 20210119
PL  - Italy
TA  - Ital J Food Saf
JT  - Italian journal of food safety
JID - 101684120
PMC - PMC7844583
OTO - NOTNLM
OT  - Enterococcus
OT  - antimicrobial resistance
OT  - cow milk
OT  - sheep milk
COIS- Conflict of interests: The authors declare no conflict of interest.
EDAT- 2021/02/04 06:00
MHDA- 2021/02/04 06:01
PMCR- 2021/01/19
CRDT- 2021/02/03 05:59
PHST- 2019/07/16 00:00 [received]
PHST- 2020/02/04 00:00 [accepted]
PHST- 2021/02/03 05:59 [entrez]
PHST- 2021/02/04 06:00 [pubmed]
PHST- 2021/02/04 06:01 [medline]
PHST- 2021/01/19 00:00 [pmc-release]
AID - 10.4081/ijfs.2020.8406 [doi]
PST - epublish
SO  - Ital J Food Saf. 2021 Jan 19;9(4):8406. doi: 10.4081/ijfs.2020.8406. eCollection 
      2020 Dec 3.