PMID- 33533122
OWN - NLM
STAT- MEDLINE
DCOM- 20220228
LR  - 20220228
IS  - 1520-6033 (Electronic)
IS  - 1520-6033 (Linking)
VI  - 37
IP  - 3
DP  - 2021 May
TI  - Two-step sedimentation process for selection of microcapsules containing cell 
      aggregates.
PG  - e3133
LID - 10.1002/btpr.3133 [doi]
AB  - Microencapsulation technologies are being developed to protect transplanted 
      islets from immune rejection, to reduce or even eliminate the need for 
      immunosuppression. However, unencapsulated cells increase the chances of 
      rejection and empty beads increase transplant volumes. Thus, separation processes 
      were investigated to remove these byproducts based on density differences. The 
      densities of islet-sized mouse insulinoma 6 (MIN6) cell aggregates and acellular 
      5% alginate beads generated via emulsification and internal gelation were ~ 1.065 
      and 1.042 g/ml, respectively. The separation of empty beads from those containing 
      aggregates was performed by sedimentation under unit gravity in continuous 
      gradients of polysucrose and sodium diatrizoate with density ranges of 
      1.032-1.045, 1.035-1.044, or 1.039-1.042 g/ml. The 1.039-1.042 g/ml gradient 
      enabled recoveries of ~ 80% of the aggregate-containing beads while the other 
      gradients recovered only ~ 60%. The bottom fraction of the 1.039-1.042 g/ml 
      gradient contained beads with ~ 6% of their volume occupied by cell aggregates. 
      Separation of unencapsulated aggregates from the aggregate-containing beads was 
      then achieved by centrifugation of this purified fraction in a 1.055 g/ml density 
      solution. Thus, these sedimentation-based approaches can effectively remove the 
      byproducts of cell encapsulation.
CI  - (c) 2021 American Institute of Chemical Engineers.
FAU - Pedroza, Rene G
AU  - Pedroza RG
AUID- ORCID: 0000-0003-3927-8280
AD  - Michael Smith Laboratories, University of British Columbia (UBC), Vancouver, 
      British Columbia, Canada.
AD  - School of Biomedical Engineering, University of British Columbia, Vancouver, 
      British Columbia, Canada.
AD  - Department of Chemical and Biological Engineering, University of British 
      Columbia, Vancouver, British Columbia, Canada.
FAU - Rajani, Sarah
AU  - Rajani S
AUID- ORCID: 0000-0002-4377-4271
AD  - Michael Smith Laboratories, University of British Columbia (UBC), Vancouver, 
      British Columbia, Canada.
AD  - Department of Chemical and Biological Engineering, University of British 
      Columbia, Vancouver, British Columbia, Canada.
FAU - Piret, James M
AU  - Piret JM
AUID- ORCID: 0000-0001-7934-9675
AD  - Michael Smith Laboratories, University of British Columbia (UBC), Vancouver, 
      British Columbia, Canada.
AD  - School of Biomedical Engineering, University of British Columbia, Vancouver, 
      British Columbia, Canada.
AD  - Department of Chemical and Biological Engineering, University of British 
      Columbia, Vancouver, British Columbia, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210216
PL  - United States
TA  - Biotechnol Prog
JT  - Biotechnology progress
JID - 8506292
SB  - IM
MH  - Animals
MH  - *Cell Encapsulation
MH  - Cell Line, Tumor
MH  - Cell Separation/*methods
MH  - Cells, Cultured
MH  - Centrifugation/*methods
MH  - Islets of Langerhans/*cytology
MH  - Mice
OTO - NOTNLM
OT  - density gradient separation
OT  - islet transplantation
OT  - microencapsulation
OT  - unencapsulated cells
EDAT- 2021/02/04 06:00
MHDA- 2022/03/01 06:00
CRDT- 2021/02/03 06:05
PHST- 2021/01/21 00:00 [revised]
PHST- 2020/11/09 00:00 [received]
PHST- 2021/01/22 00:00 [accepted]
PHST- 2021/02/04 06:00 [pubmed]
PHST- 2022/03/01 06:00 [medline]
PHST- 2021/02/03 06:05 [entrez]
AID - 10.1002/btpr.3133 [doi]
PST - ppublish
SO  - Biotechnol Prog. 2021 May;37(3):e3133. doi: 10.1002/btpr.3133. Epub 2021 Feb 16.