PMID- 33533935
OWN - NLM
STAT- MEDLINE
DCOM- 20210921
LR  - 20211006
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 220
IP  - 4
DP  - 2021 Apr 5
TI  - Dendritic cell actin dynamics control contact duration and priming efficiency at 
      the immunological synapse.
LID - 10.1083/jcb.202006081 [doi]
LID - e202006081
AB  - Dendritic cells (DCs) are crucial for the priming of naive T cells and the 
      initiation of adaptive immunity. Priming is initiated at a heterologous cell-cell 
      contact, the immunological synapse (IS). While it is established that F-actin 
      dynamics regulates signaling at the T cell side of the contact, little is known 
      about the cytoskeletal contribution on the DC side. Here, we show that the DC 
      actin cytoskeleton is decisive for the formation of a multifocal synaptic 
      structure, which correlates with T cell priming efficiency. DC actin at the IS 
      appears in transient foci that are dynamized by the WAVE regulatory complex 
      (WRC). The absence of the WRC in DCs leads to stabilized contacts with T cells, 
      caused by an increase in ICAM1-integrin-mediated cell-cell adhesion. This results 
      in lower numbers of activated and proliferating T cells, demonstrating an 
      important role for DC actin in the regulation of immune synapse functionality.
CI  - (c) 2021 Leithner et al.
FAU - Leithner, Alexander
AU  - Leithner A
AD  - Institute of Science and Technology Austria, Klosterneuburg, Austria.
AD  - Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK.
FAU - Altenburger, Lukas M
AU  - Altenburger LM
AD  - Department of Oncology, Microbiology and Immunology, University of Fribourg, 
      Fribourg, Switzerland.
FAU - Hauschild, Robert
AU  - Hauschild R
AD  - Institute of Science and Technology Austria, Klosterneuburg, Austria.
FAU - Assen, Frank P
AU  - Assen FP
AD  - Institute of Science and Technology Austria, Klosterneuburg, Austria.
FAU - Rottner, Klemens
AU  - Rottner K
AD  - Zoological Institute, Technical University Braunschweig, Braunschweig, Germany.
AD  - Department of Cell Biology, Helmholtz Centre for Infection Research, 
      Braunschweig, Germany.
FAU - Stradal, Theresia E B
AU  - Stradal TEB
AD  - Department of Cell Biology, Helmholtz Centre for Infection Research, 
      Braunschweig, Germany.
FAU - Diz-Munoz, Alba
AU  - Diz-Munoz A
AD  - Cell Biology and Biophysics Units, European Molecular Biology Laboratory, 
      Heidelberg, Germany.
FAU - Stein, Jens V
AU  - Stein JV
AD  - Department of Oncology, Microbiology and Immunology, University of Fribourg, 
      Fribourg, Switzerland.
FAU - Sixt, Michael
AU  - Sixt M
AD  - Institute of Science and Technology Austria, Klosterneuburg, Austria.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Actins)
RN  - 0 (Icam1 protein, mouse)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Actins/genetics/*immunology
MH  - Animals
MH  - Cell Adhesion/genetics/immunology
MH  - Cell Communication/genetics/*immunology
MH  - Cell Proliferation/genetics
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Immunological Synapses/genetics/*immunology
MH  - Intercellular Adhesion Molecule-1/genetics/immunology
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - T-Lymphocytes/*immunology
PMC - PMC7863705
EDAT- 2021/02/04 06:00
MHDA- 2021/09/22 06:00
PMCR- 2021/10/05
CRDT- 2021/02/03 12:13
PHST- 2020/06/13 00:00 [received]
PHST- 2020/11/25 00:00 [revised]
PHST- 2021/01/12 00:00 [accepted]
PHST- 2021/02/03 12:13 [entrez]
PHST- 2021/02/04 06:00 [pubmed]
PHST- 2021/09/22 06:00 [medline]
PHST- 2021/10/05 00:00 [pmc-release]
AID - 211749 [pii]
AID - jcb.202006081 [pii]
AID - 10.1083/jcb.202006081 [doi]
PST - ppublish
SO  - J Cell Biol. 2021 Apr 5;220(4):e202006081. doi: 10.1083/jcb.202006081.