PMID- 33534551 OWN - NLM STAT- MEDLINE DCOM- 20210621 LR - 20240331 IS - 1520-6882 (Electronic) IS - 0003-2700 (Print) IS - 0003-2700 (Linking) VI - 93 IP - 7 DP - 2021 Feb 23 TI - Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies. PG - 3576-3585 LID - 10.1021/acs.analchem.0c05082 [doi] AB - Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry (MS)-based measurements. EME allowed drugs and drug metabolites to be separated from cell medium components (albumin, etc.) that could interfere with subsequent measurements. Multiwell EME (parallel-EME) holding 100 muL solutions allowed for simple and repeatable monitoring of heroin phase I metabolism kinetics. Organoid parallel-EME extracts were compatible with ultrahigh-performance liquid chromatography (UHPLC) used to separate the analytes prior to detection. Taken together, liver organoids are well-matched with EME followed by MS-based measurements. FAU - Skottvoll, Froydis Sved AU - Skottvoll FS AUID- ORCID: 0000-0002-5299-2502 AD - Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway. AD - Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway. FAU - Hansen, Frederik Andre AU - Hansen FA AUID- ORCID: 0000-0002-1666-0447 AD - Department of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, NO-0316 Oslo, Norway. FAU - Harrison, Sean AU - Harrison S AD - Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway. FAU - Boger, Ida Sneis AU - Boger IS AD - Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway. AD - Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway. FAU - Mrsa, Ago AU - Mrsa A AD - Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway. AD - Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway. FAU - Restan, Magnus Saed AU - Restan MS AD - Department of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, NO-0316 Oslo, Norway. FAU - Stein, Matthias AU - Stein M AD - Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Beethovenstr. 55, DE-38106 Braunschweig, Germany. FAU - Lundanes, Elsa AU - Lundanes E AD - Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway. FAU - Pedersen-Bjergaard, Stig AU - Pedersen-Bjergaard S AUID- ORCID: 0000-0002-1666-8043 AD - Department of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, NO-0316 Oslo, Norway. AD - Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark. FAU - Aizenshtadt, Aleksandra AU - Aizenshtadt A AD - Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway. FAU - Krauss, Stefan AU - Krauss S AD - Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway. AD - Department of Immunology and Transfusion Medicine, Oslo University Hospital, P.O. Box 1110, Blindern, 0317, Oslo, Norway. FAU - Sullivan, Gareth AU - Sullivan G AD - Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway. AD - Department of Pediatric Research, Oslo University Hospital and University of Oslo, P.O. Box 1112, Blindern, 0317 Oslo, Norway. FAU - Bogen, Inger Lise AU - Bogen IL AD - Section for Drug Abuse Research, Department of Forensic Sciences, Oslo University Hospital, P.O. Box 4950, Nydalen, NO-0424 Oslo, Norway. AD - Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1103, Blindern, NO-0317 Oslo, Norway. FAU - Wilson, Steven Ray AU - Wilson SR AUID- ORCID: 0000-0002-9755-1188 AD - Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway. AD - Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210203 PL - United States TA - Anal Chem JT - Analytical chemistry JID - 0370536 RN - 0 (Membranes, Artificial) RN - 0 (Pharmaceutical Preparations) SB - IM MH - Liver MH - Mass Spectrometry MH - Membranes, Artificial MH - *Organoids MH - *Pharmaceutical Preparations PMC - PMC8023518 COIS- The authors declare no competing financial interest. EDAT- 2021/02/04 06:00 MHDA- 2021/06/22 06:00 PMCR- 2021/04/06 CRDT- 2021/02/03 17:09 PHST- 2021/02/04 06:00 [pubmed] PHST- 2021/06/22 06:00 [medline] PHST- 2021/02/03 17:09 [entrez] PHST- 2021/04/06 00:00 [pmc-release] AID - 10.1021/acs.analchem.0c05082 [doi] PST - ppublish SO - Anal Chem. 2021 Feb 23;93(7):3576-3585. doi: 10.1021/acs.analchem.0c05082. Epub 2021 Feb 3.