PMID- 33535103
OWN - NLM
STAT- MEDLINE
DCOM- 20210510
LR  - 20210510
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 545
DP  - 2021 Mar 19
TI  - Asarylaldehyde enhances osteogenic differentiation of human periodontal ligament 
      stem cells through the ERK/p38 MAPK signaling pathway.
PG  - 27-32
LID - S0006-291X(21)00103-0 [pii]
LID - 10.1016/j.bbrc.2021.01.053 [doi]
AB  - Periodontitis is an inflammatory disease that affects tooth-supporting tissues. 
      Chronic inflammation can progress to periodontitis, which results in loss of 
      alveolar bone. Asarylaldehyde is a potential substance for bone metabolism 
      present in natural compounds. Here, we propose the application of asarylaldehyde 
      in the osteogenic differentiation of human periodontal ligament stem cells 
      (hPDLSCs) to prevent bone loss. We investigated the effect of asarylaldehyde on 
      hPDLSCs together with bone differentiation media in vitro. The osteogenic 
      differentiation effect was observed after treatment of hPDLSCs with several 
      concentrations of asarylaldehyde. After 21 days, osteogenic cells were identified 
      by mineralization. We also observed that asarylaldehyde increased the mRNA 
      expression of osteoblast-specific markers in hPDLSCs. Interestingly, 
      asarylaldehyde regulated the levels of alkaline phosphatase (ALP) transcriptional 
      activity through the p38/extracellular-signal-regulated kinase (ERK) signaling 
      pathway. Notably, asarylaldehyde induced hPDLSCs to promote osteogenic 
      differentiation. These results suggest that asarylaldehyde plays a key role in 
      the osteogenic differentiation of hPDLSCs. Asarylaldehyde may be a good candidate 
      for the application of natural compounds in future in periodontal regeneration.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Hwang, Jin Wook
AU  - Hwang JW
AD  - INSERM U935, University Paris Saclay, 94800, Villejuif, France.
FAU - Park, Won-Jong
AU  - Park WJ
AD  - Department of Oral Pharmacology, College of Dentistry, Wonkwang University, 
      Iksan, Republic of Korea; Department of Oral and Maxillofaical Surgery, College 
      of Dentistry, Wonkwang University, Iksan, Republic of Korea. Electronic address: 
      wjpark@wku.ac.kr.
FAU - Han, Younho
AU  - Han Y
AD  - Department of Oral Pharmacology, College of Dentistry, Wonkwang University, 
      Iksan, Republic of Korea. Electronic address: daks262@wku.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210131
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Aldehydes)
RN  - NDU8J2Q00D (asaronaldehyde)
SB  - IM
MH  - Aldehydes/administration & dosage/*pharmacology
MH  - Cell Differentiation/drug effects/physiology
MH  - Cells, Cultured
MH  - Gene Expression/drug effects
MH  - Humans
MH  - MAP Kinase Signaling System/drug effects
MH  - Osteogenesis/*drug effects/genetics/physiology
MH  - Periodontal Ligament/cytology/*drug effects/metabolism
MH  - Periodontitis/drug therapy/pathology/physiopathology
MH  - Phytotherapy
MH  - Regeneration/drug effects/physiology
MH  - Stem Cells/cytology/*drug effects/metabolism
OTO - NOTNLM
OT  - Asarylaldehyde (2,4,5-trimethoxybenzaldehyde)
OT  - ERK/p38 MAPK signaling
OT  - Osteogenic differentiation
OT  - Periodontal ligament stem cells
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/02/04 06:00
MHDA- 2021/05/11 06:00
CRDT- 2021/02/03 20:06
PHST- 2020/12/28 00:00 [received]
PHST- 2021/01/19 00:00 [accepted]
PHST- 2021/02/04 06:00 [pubmed]
PHST- 2021/05/11 06:00 [medline]
PHST- 2021/02/03 20:06 [entrez]
AID - S0006-291X(21)00103-0 [pii]
AID - 10.1016/j.bbrc.2021.01.053 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2021 Mar 19;545:27-32. doi: 
      10.1016/j.bbrc.2021.01.053. Epub 2021 Jan 31.