PMID- 33535499
OWN - NLM
STAT- MEDLINE
DCOM- 20211102
LR  - 20211102
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 10
IP  - 2
DP  - 2021 Feb 1
TI  - Expression of Mst89B and CG31287 is Needed for Effective Sperm Storage and Egg 
      Fertilization in Drosophila.
LID - 10.3390/cells10020289 [doi]
LID - 289
AB  - In Drosophila, male reproductive fitness can be affected by any number of 
      processes, ranging from development of gametes, transfer to and storage of mature 
      sperm within the female sperm storage organs, and utilization of sperm for 
      fertilization. We have previously identified the 89B cytogenetic map position of 
      D. melanogaster as a hub for genes that effect male paternity success when 
      disturbed. Here, we used RNA interference to test 11 genes that are highly 
      expressed in the testes and located within the 89B region for their role in sperm 
      competition and male fecundity when their expression is perturbed. 
      Testes-specific knockdown (KD) of bor and CSN5 resulted in complete sterility, 
      whereas KD of CG31287, Manf and Mst89B, showed a breakdown in sperm competitive 
      success when second to mate (P2 < 0.5) and reduced fecundity in single matings. 
      The low fecundity of Manf KD is explained by a significant reduction in the 
      amount of mature sperm produced. KD of Mst89B and CG31287 does not affect sperm 
      production, sperm transfer into the female bursa or storage within 30 min after 
      mating. Instead, a significant reduction of sperm in female storage is observed 
      24 h after mating. Egg hatchability 24 h after mating is also drastically reduced 
      for females mated to Mst89B or CG31287 KD males, and this reduction parallels the 
      decrease in fecundity. We show that normal germ-line expression of Mst89B and 
      CG31287 is needed for effective sperm usage and egg fertilization.
FAU - Grewal, Gurman
AU  - Grewal G
AD  - Department of Biology, University of Winnipeg, Winnipeg, MB R3B 2E9, Canada.
FAU - Patlar, Bahar
AU  - Patlar B
AUID- ORCID: 0000-0002-0442-9061
AD  - Department of Biology, University of Winnipeg, Winnipeg, MB R3B 2E9, Canada.
FAU - Civetta, Alberto
AU  - Civetta A
AD  - Department of Biology, University of Winnipeg, Winnipeg, MB R3B 2E9, Canada.
LA  - eng
SI  - Dryad/10.5061/dryad.jh9w0vt9w
GR  - RGPIN-2017-04599/Natural Sciences and Engineering Research Council of Canada/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210201
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Drosophila Proteins)
SB  - IM
MH  - Animals
MH  - Drosophila/*genetics/*physiology
MH  - Drosophila Proteins/*genetics
MH  - Female
MH  - Fertility/*physiology
MH  - Fertilization/*physiology
MH  - Gene Expression
MH  - Male
MH  - Spermatozoa
MH  - Zygote/*physiology
PMC - PMC7912738
OTO - NOTNLM
OT  - fecundity
OT  - fertilization
OT  - gene expression
OT  - reproduction
OT  - sperm competition
OT  - sperm gene function
COIS- The authors declare no conflict of interest.
EDAT- 2021/02/05 06:00
MHDA- 2021/11/03 06:00
PMCR- 2021/02/01
CRDT- 2021/02/04 01:01
PHST- 2020/12/07 00:00 [received]
PHST- 2021/01/26 00:00 [revised]
PHST- 2021/01/28 00:00 [accepted]
PHST- 2021/02/04 01:01 [entrez]
PHST- 2021/02/05 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
PHST- 2021/02/01 00:00 [pmc-release]
AID - cells10020289 [pii]
AID - cells-10-00289 [pii]
AID - 10.3390/cells10020289 [doi]
PST - epublish
SO  - Cells. 2021 Feb 1;10(2):289. doi: 10.3390/cells10020289.