PMID- 33536364
OWN - NLM
STAT- MEDLINE
DCOM- 20210823
LR  - 20210823
IS  - 2234-3814 (Electronic)
IS  - 2234-3806 (Print)
IS  - 2234-3806 (Linking)
VI  - 41
IP  - 4
DP  - 2021 Jul 1
TI  - Causes of Positive Pretransplant Crossmatches in the Absence of Donor-Specific 
      Anti-Human Leukocyte Antigen Antibodies: A Single-Center Experience.
PG  - 429-435
LID - 10.3343/alm.2021.41.4.429 [doi]
AB  - Pretransplant crossmatch (XM) testing is widely used for detecting preformed 
      donor-specific antibodies (DSAs) against human leukocyte antigen (HLA). However, 
      in some cases, there is a positive XM result in the absence of HLA-DSAs, the 
      cause of which was rarely identified. We reviewed the causes of sequential 
      positive XM results at a single center and analyzed the presence of non-HLA 
      antibodies in patients with an unexplained positive pretransplant XM result. 
      Among 251 patients with T-cell/B-cell complement-dependent cytotoxicity (CDC) or 
      flow cytometric crossmatch (FCXM) positivity, HLA-DSAs were confirmed in 88 
      (35.1%) by a single antigen bead (SAB) assay, 150 (59.8%) used rituximab 
      (anti-CD20), and 13 (5.2%) had neither HLA-DSAs nor a desensitization history. 
      Anti-angiotensin II type 1 receptor IgG and 33 non-HLA antibodies were tested in 
      the 13 patients with an unexplained positive pretransplant XM result, and more 
      than one non-HLA antibody were revealed in all these patients; 11 patients had 
      non-HLA antibodies reported to be associated with graft rejection, and two 
      patients experienced rejection episode after kidney transplantation. Our study 
      suggests considering non-HLA antibodies testing when a CDC or FCXM test is 
      positive without a definite cause. Assessing non-HLA antibodies might be useful 
      for interpreting XM results and evaluating immunologic risk in transplant 
      recipients.
FAU - Kang, Hyunhye
AU  - Kang H
AUID- ORCID: 0000-0003-1338-6451
AD  - Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of 
      Medicine, The Catholic University of Korea, Seoul, Korea.
FAU - Yoo, Jaeeun
AU  - Yoo J
AUID- ORCID: 0000-0001-9917-5393
AD  - Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of 
      Medicine, The Catholic University of Korea, Seoul, Korea.
FAU - Lee, Sang-Yoon
AU  - Lee SY
AUID- ORCID: 0000-0002-1251-2877
AD  - Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of 
      Medicine, The Catholic University of Korea, Seoul, Korea.
FAU - Oh, Eun-Jee
AU  - Oh EJ
AUID- ORCID: 0000-0001-5870-915X
AD  - Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of 
      Medicine, The Catholic University of Korea, Seoul, Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Ann Lab Med
JT  - Annals of laboratory medicine
JID - 101571172
RN  - 0 (ARHGDIB protein, human)
RN  - 0 (CHAF1B protein, human)
RN  - 0 (Chromatin Assembly Factor-1)
RN  - 0 (HLA Antigens)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoprotein K)
RN  - 0 (Isoantibodies)
RN  - 0 (rho Guanine Nucleotide Dissociation Inhibitor beta)
RN  - 146410-60-8 (HNRNPK protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromatin Assembly Factor-1
MH  - Female
MH  - Flow Cytometry
MH  - Graft Rejection
MH  - HLA Antigens
MH  - Heterogeneous-Nuclear Ribonucleoprotein K
MH  - Histocompatibility Testing
MH  - Humans
MH  - Isoantibodies
MH  - *Liver Transplantation
MH  - Living Donors
MH  - Male
MH  - Middle Aged
MH  - rho Guanine Nucleotide Dissociation Inhibitor beta
PMC - PMC7884190
OTO - NOTNLM
OT  - Complement-dependent cytotoxicity
OT  - Donor-specific antibodies against human leukocyte antigen
OT  - Flow cytometric crossmatch
OT  - Transplantation
OT  - non-HLA antibodies
COIS- CONFLICTS OF INTEREST No potential conflicts of interest relevant to this article 
      are reported.
EDAT- 2021/02/05 06:00
MHDA- 2021/08/24 06:00
PMCR- 2021/07/01
CRDT- 2021/02/04 05:41
PHST- 2020/05/14 00:00 [received]
PHST- 2020/07/20 00:00 [revised]
PHST- 2020/11/24 00:00 [accepted]
PHST- 2021/02/04 05:41 [entrez]
PHST- 2021/02/05 06:00 [pubmed]
PHST- 2021/08/24 06:00 [medline]
PHST- 2021/07/01 00:00 [pmc-release]
AID - alm.2021.41.4.429 [pii]
AID - alm-41-4-429 [pii]
AID - 10.3343/alm.2021.41.4.429 [doi]
PST - ppublish
SO  - Ann Lab Med. 2021 Jul 1;41(4):429-435. doi: 10.3343/alm.2021.41.4.429.