PMID- 33536463
OWN - NLM
STAT- MEDLINE
DCOM- 20211115
LR  - 20211115
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Feb 3
TI  - An evaluation of multi-excitation-wavelength standing-wave fluorescence 
      microscopy (TartanSW) to improve sampling density in studies of the cell membrane 
      and cytoskeleton.
PG  - 2903
LID - 10.1038/s41598-020-78282-6 [doi]
LID - 2903
AB  - Conventional standing-wave (SW) fluorescence microscopy uses a single wavelength 
      to excite fluorescence from the specimen, which is normally placed in contact 
      with a first surface reflector. The resulting excitation SW creates a pattern of 
      illumination with anti-nodal maxima at multiple evenly-spaced planes 
      perpendicular to the optical axis of the microscope. These maxima are 
      approximately 90 nm thick and spaced 180 nm apart. Where the planes intersect 
      fluorescent structures, emission occurs, but between the planes are 
      non-illuminated regions which are not sampled for fluorescence. We evaluate a 
      multi-excitation-wavelength SW fluorescence microscopy (which we call TartanSW) 
      as a method for increasing the density of sampling by using SWs with different 
      axial periodicities, to resolve more of the overall cell structure. The TartanSW 
      method increased the sampling density from 50 to 98% over seven anti-nodal 
      planes, with no notable change in axial or lateral resolution compared to 
      single-excitation-wavelength SW microscopy. We demonstrate the method with images 
      of the membrane and cytoskeleton of living and fixed cells.
FAU - Schniete, Jana K
AU  - Schniete JK
AD  - Department of Physics, SUPA, University of Strathclyde, 107 Rottenrow East, 
      Glasgow, G4 0NG, UK. jana.schniete@strath.ac.uk.
FAU - Tinning, Peter W
AU  - Tinning PW
AD  - Department of Physics, SUPA, University of Strathclyde, 107 Rottenrow East, 
      Glasgow, G4 0NG, UK.
FAU - Scrimgeour, Ross C
AU  - Scrimgeour RC
AD  - Department of Physics, SUPA, University of Strathclyde, 107 Rottenrow East, 
      Glasgow, G4 0NG, UK.
FAU - Robb, Gillian
AU  - Robb G
AD  - Department of Physics, SUPA, University of Strathclyde, 107 Rottenrow East, 
      Glasgow, G4 0NG, UK.
FAU - Kolln, Lisa S
AU  - Kolln LS
AD  - Department of Physics, SUPA, University of Strathclyde, 107 Rottenrow East, 
      Glasgow, G4 0NG, UK.
FAU - Wesencraft, Katrina
AU  - Wesencraft K
AD  - Department of Physics, SUPA, University of Strathclyde, 107 Rottenrow East, 
      Glasgow, G4 0NG, UK.
FAU - Paul, Nikki R
AU  - Paul NR
AD  - Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Bearsden, 
      Glasgow, G61 1BD, UK.
FAU - Bushell, Trevor J
AU  - Bushell TJ
AD  - Strathclyde Institute of Pharmacy and Biomedical Sciences, University of 
      Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK.
FAU - McConnell, Gail
AU  - McConnell G
AD  - Department of Physics, SUPA, University of Strathclyde, 107 Rottenrow East, 
      Glasgow, G4 0NG, UK.
LA  - eng
GR  - BB/P02565X/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - MR/K015583/1/MRC_/Medical Research Council/United Kingdom
GR  - MR/R017247/1/MRC_/Medical Research Council/United Kingdom
GR  - MR/K015583/1/MRC_/Medical Research Council/United Kingdom
GR  - EP/L016559/1/EPSRC Centre for Doctoral Training in Medical Imaging/
GR  - A17196/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210203
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - *Cell Membrane
MH  - *Cytoskeleton
MH  - Humans
MH  - Image Enhancement/instrumentation/*methods
MH  - Intravital Microscopy/instrumentation/*methods
MH  - Mice
MH  - Microscopy, Fluorescence/instrumentation/methods
PMC - PMC7858599
COIS- The authors declare no competing interests.
EDAT- 2021/02/05 06:00
MHDA- 2021/11/16 06:00
PMCR- 2021/02/03
CRDT- 2021/02/04 05:45
PHST- 2020/05/21 00:00 [received]
PHST- 2020/11/23 00:00 [accepted]
PHST- 2021/02/04 05:45 [entrez]
PHST- 2021/02/05 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
PHST- 2021/02/03 00:00 [pmc-release]
AID - 10.1038/s41598-020-78282-6 [pii]
AID - 78282 [pii]
AID - 10.1038/s41598-020-78282-6 [doi]
PST - epublish
SO  - Sci Rep. 2021 Feb 3;11(1):2903. doi: 10.1038/s41598-020-78282-6.