PMID- 33537343 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210206 IS - 2296-889X (Print) IS - 2296-889X (Electronic) IS - 2296-889X (Linking) VI - 7 DP - 2020 TI - CRISPR-Cas13-Mediated Knockdown of lncRNA-GACAT3 Inhibited Cell Proliferation and Motility, and Induced Apoptosis by Increasing p21, Bax, and E-Cadherin Expression in Bladder Cancer. PG - 627774 LID - 10.3389/fmolb.2020.627774 [doi] LID - 627774 AB - The current study is to investigate the expression pattern and biological function of long non-coding RNA Focally gastric cancer-associated transcript3 (GACAT3) in bladder cancer. Real-time quantitative qPCR was used to detect the expression level of GACAT-3 in tumor tissues and paired normal tissues. Human bladder cancer T24 and 5637 cell lines were transiently transfected with specific CRISPR-Cas13 or negative control CRISPR-Cas13. Cell migration, proliferation, and apoptosis were measured by using wound healing assay CCK-8 assay and Caspase-3 ELISA assay, respectively. The expression changes of p21, Bax, and E-cadherin after knockdown of GACAT3 were detected by using Western blot. The results demonstrated that GACAT3 was up-regulated in bladder cancer tissues than that in the paired normal tissues. Inhibition of cell proliferation, increased apoptosis, and decreased motility were observed in T24 and 5637 cell lines transfected by CRISPR-Cas13 targeting GACAT3. Downregulation of GACAT3 increased p21, Bax, and E-cadherin expression and silencing these genes could eliminate the phenotypic changes induced by knockdown of GACAT3. A ceRNA mechanism for GACAT3 was also revealed. By using CRISPR-Cas13 biotechnology, we suggested that GACAT3 may be a novel target for diagnosis and treatment of bladder cancer. CI - Copyright (c) 2021 Zhang, Chen, Zhu, Zhu, Liao, Wu, Cheng, Zhang, Mei and Yang. FAU - Zhang, Zhongfu AU - Zhang Z AD - The Second School of Clinical Medicine, Southern Medical University Affiliated Guangdong Second Provincial General Hospital, Southern Medical University, Guangzhou, China. AD - Department of Urology, Guangdong Second Provincial General Hospital, Guangzhou, China. AD - Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. AD - Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. FAU - Chen, Jieqing AU - Chen J AD - Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. AD - Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. FAU - Zhu, Zhongshuang AU - Zhu Z AD - Peking University Shenzhen Hospital, Shenzhen, China. FAU - Zhu, Zhongqing AU - Zhu Z AD - Hong Kong University Shenzhen Hospital, Shenzhen, China. FAU - Liao, Xinhui AU - Liao X AD - Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. AD - Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. FAU - Wu, Jianting AU - Wu J AD - Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. AD - Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. FAU - Cheng, Jianli AU - Cheng J AD - Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. AD - Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. FAU - Zhang, Xintao AU - Zhang X AD - Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. AD - Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. FAU - Mei, Hongbing AU - Mei H AD - Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. AD - Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China. FAU - Yang, Guosheng AU - Yang G AD - The Second School of Clinical Medicine, Southern Medical University Affiliated Guangdong Second Provincial General Hospital, Southern Medical University, Guangzhou, China. AD - Department of Urology, Guangdong Second Provincial General Hospital, Guangzhou, China. AD - Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China. LA - eng PT - Journal Article DEP - 20210118 PL - Switzerland TA - Front Mol Biosci JT - Frontiers in molecular biosciences JID - 101653173 PMC - PMC7848205 OTO - NOTNLM OT - CRISPR-Cas13 OT - GACAT3 OT - bladder cancer OT - cancer development OT - long non-coding RNA COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2021/02/05 06:00 MHDA- 2021/02/05 06:01 PMCR- 2020/01/01 CRDT- 2021/02/04 05:52 PHST- 2020/11/10 00:00 [received] PHST- 2020/11/27 00:00 [accepted] PHST- 2021/02/04 05:52 [entrez] PHST- 2021/02/05 06:00 [pubmed] PHST- 2021/02/05 06:01 [medline] PHST- 2020/01/01 00:00 [pmc-release] AID - 10.3389/fmolb.2020.627774 [doi] PST - epublish SO - Front Mol Biosci. 2021 Jan 18;7:627774. doi: 10.3389/fmolb.2020.627774. eCollection 2020.