PMID- 33538066
OWN - NLM
STAT- MEDLINE
DCOM- 20220216
LR  - 20220216
IS  - 1470-8744 (Electronic)
IS  - 0885-4513 (Linking)
VI  - 69
IP  - 1
DP  - 2022 Feb
TI  - Palbociclib negatively regulates fatty acid synthesis due to upregulation of 
      AMPKalpha and miR-33a levels to increase apoptosis in Panc-1 and MiaPaCa-2 cells.
PG  - 342-354
LID - 10.1002/bab.2113 [doi]
AB  - Fatty acids (FAs) synthesis mechanism has various regulators such as fatty acid 
      synthase (FASN), AMP-regulated protein kinase (AMPK), or mammalian target of 
      rapamycin (mTOR), which are aberrantly dysregulated in various pancreatic cancer 
      cells. In this study, we aim to understand the regulatory role of palbociclib, a 
      CDK4/6 inhibitor, on the cellular energy metabolism through regulation of 
      AMPK/mTOR signaling by modulation of intracellular miR-33a levels in Panc-1 and 
      MiaPaCa-2 cells. Palbociclib downregulated FAs metabolism more effectively in 
      MiaPaCa-2 cells than Panc-1 cells. Moreover, palbociclib treatment increased the 
      levels of miR-33a in each cell line albeit a higher increase was evident in 
      MiaPaCa-2 cells. Stress-mediated activation of mTOR signaling axis was found 
      associated with palbociclib-mediated AMPKalpha activation and miR33a upregulation. 
      These findings provided that a deeper understanding about possible interactions 
      of cell cycle activity and reduction of FAs synthesis may facilitate the 
      enhancement of cell death mechanisms in pancreatic cancer cells.
CI  - (c) 2021 International Union of Biochemistry and Molecular Biology, Inc.
FAU - Rencuzogullari, Ozge
AU  - Rencuzogullari O
AUID- ORCID: 0000-0002-2157-1289
AD  - Department of Molecular Biology and Genetics, Science and Literature Faculty, 
      Istanbul Kultur University, Istanbul, Turkey.
AD  - Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe 
      University, Istanbul, Turkey.
FAU - Yerlikaya, Pinar Obakan
AU  - Yerlikaya PO
AUID- ORCID: 0000-0001-7058-955X
AD  - Department of Molecular Biology and Genetics, Science and Literature Faculty, 
      Istanbul Kultur University, Istanbul, Turkey.
FAU - Gurkan, Ajda Coker
AU  - Gurkan AC
AUID- ORCID: 0000-0003-2298-5755
AD  - Department of Molecular Biology and Genetics, Science and Literature Faculty, 
      Istanbul Kultur University, Istanbul, Turkey.
FAU - Arisan, Elif Damla
AU  - Arisan ED
AUID- ORCID: 0000-0002-4844-6381
AD  - Department of Biotechnology, Institute of Biotechnology, Gebze Technical 
      University, Gebze, Kocaeli, Turkey.
FAU - Telci, Dilek
AU  - Telci D
AUID- ORCID: 0000-0002-2793-6533
AD  - Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe 
      University, Istanbul, Turkey.
LA  - eng
GR  - 000x/Istanbul Kultur University Scientific Research Center/
GR  - 119Z158/Turkiye Bilimsel ve Teknolojik Arastirma Kurumu/
GR  - 118Z100/Turkiye Bilimsel ve Teknolojik Arastirma Kurumu/
PT  - Journal Article
DEP - 20210218
PL  - United States
TA  - Biotechnol Appl Biochem
JT  - Biotechnology and applied biochemistry
JID - 8609465
RN  - 0 (Fatty Acids)
RN  - 0 (MicroRNAs)
RN  - 0 (Piperazines)
RN  - 0 (Pyridines)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - G9ZF61LE7G (palbociclib)
SB  - IM
MH  - *AMP-Activated Protein Kinases/metabolism
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Fatty Acids
MH  - *MicroRNAs/genetics
MH  - Piperazines
MH  - Pyridines
MH  - Up-Regulation
OTO - NOTNLM
OT  - AMPKalpha
OT  - fatty acid synthesis
OT  - leptin
OT  - miR-33a
OT  - palbociclib
OT  - pancreatic cancer
EDAT- 2021/02/05 06:00
MHDA- 2022/02/17 06:00
CRDT- 2021/02/04 05:57
PHST- 2020/07/21 00:00 [received]
PHST- 2021/01/10 00:00 [accepted]
PHST- 2021/02/05 06:00 [pubmed]
PHST- 2022/02/17 06:00 [medline]
PHST- 2021/02/04 05:57 [entrez]
AID - 10.1002/bab.2113 [doi]
PST - ppublish
SO  - Biotechnol Appl Biochem. 2022 Feb;69(1):342-354. doi: 10.1002/bab.2113. Epub 2021 
      Feb 18.