PMID- 33543212
OWN - NLM
STAT- MEDLINE
DCOM- 20210208
LR  - 20220531
IS  - 1006-7248 (Print)
IS  - 1006-7248 (Linking)
VI  - 29
IP  - 5
DP  - 2020 Oct
TI  - [Construction of ADAM28 shRNA interference vector and its inhibitory effect on 
      human periodontal ligament stem cells].
PG  - 476-481
AB  - PURPOSE: To investigate the inhibition of ADAM28 shRNA interfering vector on 
      ADAM28 gene expression in human periodontal ligament stem cells(HPDLSC), and 
      provide experimental evidence for gene therapy against congenital hypoplasia of 
      tooth root(CHTR) disease. METHODS: Four pairs of shRNA specific interfering 
      fragments were designed, synthesized, and connected with pGPU6/GFP/Neo vector. 
      ADAM28 shRNA interfering vector was constructed and identified, and transfected 
      into HPDLSC for 48 h, and then the inhibition efficiency was detected by 
      real-time fluorescence quantitative RT-PCR（qRT-PCR）and Western blot. Statistical 
      significance was assessed by SPSS 21.0 software package. RESULTS: Enzyme 
      digestion and sequencing identification demonstrated that the double strands 
      shRNA was correctly inserted into the expression vector pGPU6/GFP/Neo, and the 
      recombinant interfering vector was successfully constructed and highly 
      transfected into HPDLSC. QRT-PCR and Western blot showed that 
      pGPU6/GFP/Neo-ADAM28-shRNA1-4 had significant inhibition efficiency, and shRNA1 
      had the highest inhibition efficiency. There were significant differences between 
      ADAM28-shRNA1-4 group and non-transfection group, negative control group, 
      respectively(P<0.05). CONCLUSIONS: ADAM28 shRNA interference vector can 
      effectively inhibit ADAM28 gene expression in HPDLSC.
FAU - Zhao, Zheng
AU  - Zhao Z
AD  - Department of Geriatric Stomatology, Qingdao Stomatological Hospital. Qingdao 
      266001, Shandong Province, China. E-mail:zhaozheng170@163.com.
FAU - Qiu, Hai-Yan
AU  - Qiu HY
FAU - Fu, Lan
AU  - Fu L
FAU - Li, Jie
AU  - Li J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Shanghai Kou Qiang Yi Xue
JT  - Shanghai kou qiang yi xue = Shanghai journal of stomatology
JID - 101090220
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (ADAM28 protein, human)
SB  - IM
MH  - ADAM Proteins
MH  - Genetic Vectors/genetics
MH  - Humans
MH  - *Periodontal Ligament
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics
MH  - *Stem Cells
MH  - Transfection
EDAT- 2021/02/06 06:00
MHDA- 2021/02/09 06:00
CRDT- 2021/02/05 06:00
PHST- 2021/02/05 06:00 [entrez]
PHST- 2021/02/06 06:00 [pubmed]
PHST- 2021/02/09 06:00 [medline]
PST - ppublish
SO  - Shanghai Kou Qiang Yi Xue. 2020 Oct;29(5):476-481.