PMID- 33544927
OWN - NLM
STAT- MEDLINE
DCOM- 20210922
LR  - 20221207
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 106
IP  - 3
DP  - 2021 May
TI  - Cell wall-derived mixed-linked beta-1,3/1,4-glucans trigger immune responses and 
      disease resistance in plants.
PG  - 601-615
LID - 10.1111/tpj.15185 [doi]
AB  - Pattern-triggered immunity (PTI) is activated in plants upon recognition by 
      pattern recognition receptors (PRRs) of damage- and microbe-associated molecular 
      patterns (DAMPs and MAMPs) derived from plants or microorganisms, respectively. 
      To understand better the plant mechanisms involved in the perception of 
      carbohydrate-based structures recognized as DAMPs/MAMPs, we have studied the 
      ability of mixed-linked beta-1,3/1,4-glucans (MLGs), present in some plant and 
      microbial cell walls, to trigger immune responses and disease resistance in 
      plants. A range of MLG structures were tested for their capacity to induce PTI 
      hallmarks, such as cytoplasmic Ca(2+) elevations, reactive oxygen species 
      production, phosphorylation of mitogen-activated protein kinases and gene 
      transcriptional reprogramming. These analyses revealed that MLG oligosaccharides 
      are perceived by Arabidopsis thaliana and identified a trisaccharide, 
      beta-d-cellobiosyl-(1,3)-beta-d-glucose (MLG43), as the smallest MLG structure 
      triggering strong PTI responses. These MLG43-mediated PTI responses are partially 
      dependent on LysM PRRs CERK1, LYK4 and LYK5, as they were weaker in cerk1 and 
      lyk4 lyk5 mutants than in wild-type plants. Cross-elicitation experiments between 
      MLG43 and the carbohydrate MAMP chitohexaose [beta-1,4-d-(GlcNAc)(6) ], which is 
      also perceived by these LysM PRRs, indicated that the mechanism of MLG43 
      recognition could differ from that of chitohexaose, which is fully impaired in 
      cerk1 and lyk4 lyk5 plants. MLG43 treatment confers enhanced disease resistance 
      in A. thaliana to the oomycete Hyaloperonospora arabidopsidis and in tomato and 
      pepper to different bacterial and fungal pathogens. Our data support the 
      classification of MLGs as a group of carbohydrate-based molecular patterns that 
      are perceived by plants and trigger immune responses and disease resistance.
CI  - (c) 2021 The Authors. The Plant Journal published by Society for Experimental 
      Biology and John Wiley & Sons Ltd.
FAU - Rebaque, Diego
AU  - Rebaque D
AD  - Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid 
      (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria 
      (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcon (Madrid), Spain.
AD  - Departamento de Biotecnologia-Biologia Vegetal, Escuela Tecnica Superior de 
      Ingenieria Agronomica, Alimentaria y de Biosistemas, UPM, Madrid, Spain.
AD  - Plant Response Biotech, Centro de Empresas, Campus de Montegancedo UPM, Pozuelo 
      de Alarcon (Madrid), Spain.
FAU - Del Hierro, Irene
AU  - Del Hierro I
AD  - Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid 
      (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria 
      (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcon (Madrid), Spain.
AD  - Departamento de Biotecnologia-Biologia Vegetal, Escuela Tecnica Superior de 
      Ingenieria Agronomica, Alimentaria y de Biosistemas, UPM, Madrid, Spain.
FAU - Lopez, Gemma
AU  - Lopez G
AD  - Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid 
      (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria 
      (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcon (Madrid), Spain.
FAU - Bacete, Laura
AU  - Bacete L
AUID- ORCID: 0000-0003-3171-8181
AD  - Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid 
      (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria 
      (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcon (Madrid), Spain.
AD  - Departamento de Biotecnologia-Biologia Vegetal, Escuela Tecnica Superior de 
      Ingenieria Agronomica, Alimentaria y de Biosistemas, UPM, Madrid, Spain.
FAU - Vilaplana, Francisco
AU  - Vilaplana F
AD  - Division of Glycoscience, School of Biotechnology, Royal Institute of Technology 
      (KTH), Stockholm, Sweden.
FAU - Dallabernardina, Pietro
AU  - Dallabernardina P
AD  - Department of Biomolecular Systems, Max Planck Institute of Colloids and 
      Interfaces, Potsdam, Germany.
FAU - Pfrengle, Fabian
AU  - Pfrengle F
AD  - Department of Biomolecular Systems, Max Planck Institute of Colloids and 
      Interfaces, Potsdam, Germany.
FAU - Jorda, Lucia
AU  - Jorda L
AD  - Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid 
      (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria 
      (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcon (Madrid), Spain.
AD  - Departamento de Biotecnologia-Biologia Vegetal, Escuela Tecnica Superior de 
      Ingenieria Agronomica, Alimentaria y de Biosistemas, UPM, Madrid, Spain.
FAU - Sanchez-Vallet, Andrea
AU  - Sanchez-Vallet A
AD  - Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid 
      (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria 
      (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcon (Madrid), Spain.
FAU - Perez, Rosa
AU  - Perez R
AD  - Plant Response Biotech, Centro de Empresas, Campus de Montegancedo UPM, Pozuelo 
      de Alarcon (Madrid), Spain.
FAU - Brunner, Frederic
AU  - Brunner F
AD  - Plant Response Biotech, Centro de Empresas, Campus de Montegancedo UPM, Pozuelo 
      de Alarcon (Madrid), Spain.
FAU - Molina, Antonio
AU  - Molina A
AUID- ORCID: 0000-0003-3137-7938
AD  - Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid 
      (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria 
      (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcon (Madrid), Spain.
AD  - Departamento de Biotecnologia-Biologia Vegetal, Escuela Tecnica Superior de 
      Ingenieria Agronomica, Alimentaria y de Biosistemas, UPM, Madrid, Spain.
FAU - Melida, Hugo
AU  - Melida H
AUID- ORCID: 0000-0003-1792-0113
AD  - Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid 
      (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria 
      (INIA), Campus de Montegancedo UPM, Pozuelo de Alarcon (Madrid), Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210322
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Trisaccharides)
RN  - 0 (beta-Glucans)
RN  - 55965-23-6 (beta-glucan, (1-3)(1-4)-)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Arabidopsis/immunology/metabolism
MH  - Calcium/metabolism
MH  - Capsicum/immunology/metabolism
MH  - Cell Wall/*metabolism
MH  - *Disease Resistance
MH  - Solanum lycopersicum/immunology/metabolism
MH  - Oomycetes/immunology
MH  - Plant Diseases/immunology/microbiology
MH  - *Plant Immunity
MH  - Trisaccharides
MH  - beta-Glucans/*metabolism
PMC - PMC8252745
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - Capsicum annuum
OT  - Hyaloperonospora arabidopsidis
OT  - Solanum lycopersicum
OT  - cell wall
OT  - disease resistance
OT  - mixed-linked glucan
OT  - pattern triggered immunity
OT  - plant immunity
COIS- The authors declare that they have no competing interests.
EDAT- 2021/02/06 06:00
MHDA- 2021/09/23 06:00
PMCR- 2021/07/02
CRDT- 2021/02/05 17:13
PHST- 2021/01/26 00:00 [revised]
PHST- 2020/08/27 00:00 [received]
PHST- 2021/01/28 00:00 [accepted]
PHST- 2021/02/06 06:00 [pubmed]
PHST- 2021/09/23 06:00 [medline]
PHST- 2021/02/05 17:13 [entrez]
PHST- 2021/07/02 00:00 [pmc-release]
AID - TPJ15185 [pii]
AID - 10.1111/tpj.15185 [doi]
PST - ppublish
SO  - Plant J. 2021 May;106(3):601-615. doi: 10.1111/tpj.15185. Epub 2021 Mar 22.