PMID- 33545389
OWN - NLM
STAT- MEDLINE
DCOM- 20210524
LR  - 20210524
IS  - 1556-1380 (Electronic)
IS  - 1556-0864 (Linking)
VI  - 16
IP  - 5
DP  - 2021 May
TI  - Prospective Multicenter Validation of the Detection of ALK Rearrangements of 
      Circulating Tumor Cells for Noninvasive Longitudinal Management of Patients With 
      Advanced NSCLC.
PG  - 807-816
LID - S1556-0864(21)01664-6 [pii]
LID - 10.1016/j.jtho.2021.01.1617 [doi]
AB  - INTRODUCTION: Patients with advanced-stage NSCLC whose tumors harbor an ALK gene 
      rearrangement benefit from treatment with multiple ALK inhibitors (ALKi). 
      Approximately 30% of tumor biopsy samples contain insufficient tissue for 
      successful ALK molecular characterization. This study evaluated the added value 
      of analyzing circulating tumor cells (CTCs) as a surrogate to ALK tissue analysis 
      and as a function of the response to ALKi. METHODS: We conducted a multicenter, 
      prospective observational study (NCT02372448) of 203 patients with stage IIIB/IV 
      NSCLC across nine French centers, of whom 81 were ALK positive 
      (immunohistochemistry or fluorescence in situ hybridization [FISH]) and 122 ALK 
      negative on paraffin-embedded tissue specimens. Blood samples were collected at 
      baseline and at 6 and 12 weeks after ALKi initiation or at disease progression. 
      ALK gene rearrangement was evaluated with CTCs using immunocytochemistry and FISH 
      analysis after enrichment using a filtration method. RESULTS: At baseline, there 
      was a high concordance between the detection of an ALK rearrangement in the 
      tumor tissue and in CTCs as determined by immunocytochemistry (sensitivity, 
      94.4%; specificity 89.4%). The performance was lower for the FISH analysis 
      (sensitivity, 35.6%; specificity, 56.9%). No significant association between the 
      baseline levels or the dynamic change of CTCs and overall survival (hazard ratio 
      = 0.59, 95% confidence interval: 0.24-1.5, p = 0.244) or progression-free 
      survival (hazard ratio = 0.84, 95% confidence interval: 0.44-1.6, p = 0.591) was 
      observed in the patients with ALK-positive NSCLC. CONCLUSIONS: CTCs can be used 
      as a complementary tool to a tissue biopsy for the detection of ALK 
      rearrangements. Longitudinal analyses of CTCs revealed promise for real-time 
      patient monitoring and improved delivery of molecularly guided therapy in this 
      population.
CI  - Copyright (c) 2021 International Association for the Study of Lung Cancer. 
      Published by Elsevier Inc. All rights reserved.
FAU - Ilie, Marius
AU  - Ilie M
AD  - Laboratory of Clinical and Experimental Pathology, FHU OncoAge, Centre 
      Hospitalier Universitaire de Nice, Universite Cote d'Azur, Nice, France; 
      Hospital-Related Biobank (BB-0033-00025), FHU OncoAge, Centre Hospitalier 
      Universitaire de Nice, Universite Cote d'Azur, Nice, France; Institute of 
      Research on Cancer and Ageing of Nice (IRCAN), CNRS, INSERM, FHU OncoAge, 
      Universite Cote d'Azur, Nice, France.
FAU - Mazieres, Julien
AU  - Mazieres J
AD  - Department of Pulmonology, Centre Hospitalier Universitaire Toulouse, Institut 
      Universitaire du Cancer, Universite Paul Sabatier, Toulouse, France.
FAU - Chamorey, Emmanuel
AU  - Chamorey E
AD  - Biostatistics Unit, Antoine Lacassagne Comprehensive Cancer Center, Nice, France.
FAU - Heeke, Simon
AU  - Heeke S
AD  - Laboratory of Clinical and Experimental Pathology, FHU OncoAge, Centre 
      Hospitalier Universitaire de Nice, Universite Cote d'Azur, Nice, France; 
      Hospital-Related Biobank (BB-0033-00025), FHU OncoAge, Centre Hospitalier 
      Universitaire de Nice, Universite Cote d'Azur, Nice, France; Institute of 
      Research on Cancer and Ageing of Nice (IRCAN), CNRS, INSERM, FHU OncoAge, 
      Universite Cote d'Azur, Nice, France; Department of Thoracic/Head & Neck Medical 
      Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas.
FAU - Benzaquen, Jonathan
AU  - Benzaquen J
AD  - Institute of Research on Cancer and Ageing of Nice (IRCAN), CNRS, INSERM, FHU 
      OncoAge, Universite Cote d'Azur, Nice, France; Department of Pulmonary Medicine 
      and Oncology, FHU OncoAge, Centre Hospitalier Universitaire de Nice, Universite 
      Cote d'Azur, Nice, France.
FAU - Thamphya, Brice
AU  - Thamphya B
AD  - Biostatistics Unit, Antoine Lacassagne Comprehensive Cancer Center, Nice, France.
FAU - Boutros, Jacques
AU  - Boutros J
AD  - Department of Pulmonary Medicine and Oncology, FHU OncoAge, Centre Hospitalier 
      Universitaire de Nice, Universite Cote d'Azur, Nice, France.
FAU - Tiotiu, Angelica
AU  - Tiotiu A
AD  - Department of Pulmonology, Centre Hospitalier Regional Universitaire de Nancy, 
      Nancy, France; Development, Adaptation and Disadvantage, Cardio-Respiratory 
      Regulations and Motor Control, Universite de Lorraine, Nancy, France.
FAU - Fayada, Julien
AU  - Fayada J
AD  - Hospital-Related Biobank (BB-0033-00025), FHU OncoAge, Centre Hospitalier 
      Universitaire de Nice, Universite Cote d'Azur, Nice, France.
FAU - Cadranel, Jacques
AU  - Cadranel J
AD  - Department of Pulmonology, APHP, Hopital Tenon and GRC04 Theranoscan, Sorbonne 
      Universite, Paris, France.
FAU - Poudenx, Michel
AU  - Poudenx M
AD  - Department of Pulmonary Medicine and Oncology, FHU OncoAge, Centre Hospitalier 
      Universitaire de Nice, Universite Cote d'Azur, Nice, France; Department of 
      Oncology, Centre Antoine Lacassagne, Nice, France.
FAU - Moro-Sibilot, Denis
AU  - Moro-Sibilot D
AD  - Thoracic Oncology Unit, Centre hospitalier universitaire Grenoble-Alpes, 
      Grenoble, France.
FAU - Barlesi, Fabrice
AU  - Barlesi F
AD  - Centre d'Essais Precoces en Cancerologie de Marseille CLIP(2), Aix Marseille 
      University, CNRS, INSERM, CRCM, APHM, Marseille, France; Department of Cancer 
      Medicine, Institut Gustave Roussy, Villejuif, France.
FAU - Thariat, Juliette
AU  - Thariat J
AD  - Department of Radiation Therapy, Centre Francois Baclesse-ARCHADE, Universite de 
      Caen Normandie, Caen, France.
FAU - Clement-Duchene, Christelle
AU  - Clement-Duchene C
AD  - Oncology Department, Institut de Cancerologie de Lorraine, Nancy, France.
FAU - Tomasini, Pascale
AU  - Tomasini P
AD  - Centre d'Essais Precoces en Cancerologie de Marseille CLIP(2), Aix Marseille 
      University, CNRS, INSERM, CRCM, APHM, Marseille, France.
FAU - Hofman, Veronique
AU  - Hofman V
AD  - Laboratory of Clinical and Experimental Pathology, FHU OncoAge, Centre 
      Hospitalier Universitaire de Nice, Universite Cote d'Azur, Nice, France; 
      Hospital-Related Biobank (BB-0033-00025), FHU OncoAge, Centre Hospitalier 
      Universitaire de Nice, Universite Cote d'Azur, Nice, France; Institute of 
      Research on Cancer and Ageing of Nice (IRCAN), CNRS, INSERM, FHU OncoAge, 
      Universite Cote d'Azur, Nice, France.
FAU - Marquette, Charles-Hugo
AU  - Marquette CH
AD  - Institute of Research on Cancer and Ageing of Nice (IRCAN), CNRS, INSERM, FHU 
      OncoAge, Universite Cote d'Azur, Nice, France; Department of Pulmonary Medicine 
      and Oncology, FHU OncoAge, Centre Hospitalier Universitaire de Nice, Universite 
      Cote d'Azur, Nice, France.
FAU - Hofman, Paul
AU  - Hofman P
AD  - Laboratory of Clinical and Experimental Pathology, FHU OncoAge, Centre 
      Hospitalier Universitaire de Nice, Universite Cote d'Azur, Nice, France; 
      Hospital-Related Biobank (BB-0033-00025), FHU OncoAge, Centre Hospitalier 
      Universitaire de Nice, Universite Cote d'Azur, Nice, France; Institute of 
      Research on Cancer and Ageing of Nice (IRCAN), CNRS, INSERM, FHU OncoAge, 
      Universite Cote d'Azur, Nice, France. Electronic address: hofman.p@chu-nice.fr.
CN  - STALKLUNG01 Study Consortium Investigators
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Observational Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20210203
PL  - United States
TA  - J Thorac Oncol
JT  - Journal of thoracic oncology : official publication of the International 
      Association for the Study of Lung Cancer
JID - 101274235
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Anaplastic Lymphoma Kinase/genetics
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Lung Neoplasms/drug therapy/genetics
MH  - *Neoplastic Cells, Circulating
MH  - Prospective Studies
MH  - Receptor Protein-Tyrosine Kinases/genetics
OTO - NOTNLM
OT  - ALK
OT  - CTCs
OT  - FISH
OT  - Immunocytochemistry
OT  - Lung adenocarcinoma
FIR - Israel-Biet, Dominique
IR  - Israel-Biet D
IRAD- Hopital Europeen Georges Pompidou.
FIR - Pison, Christophe
IR  - Pison C
IRAD- Hopital Universitaire de Grenoble.
FIR - Moro-Sibilot, Denis
IR  - Moro-Sibilot D
IRAD- Hopital Universitaire de Grenoble.
FIR - Lantuejoul, Sylvie
IR  - Lantuejoul S
IRAD- Hopital Universitaire de Grenoble.
FIR - Stephanov, Olivier
IR  - Stephanov O
IRAD- Hopital Universitaire de Grenoble.
FIR - Juliette, Meyzenc
IR  - Juliette M
IRAD- Hopital Universitaire de Grenoble.
FIR - Mendozat, Christophe
IR  - Mendozat C
IRAD- Hopital Universitaire de Grenoble.
FIR - Zaidi, Manel
IR  - Zaidi M
IRAD- Hopital Universitaire de Grenoble.
FIR - Coulouvrat, Sandra
IR  - Coulouvrat S
FIR - Col, Edwige
IR  - Col E
IRAD- Hopital Universitaire de Grenoble.
FIR - Chanez, Pascal
IR  - Chanez P
IRAD- APHM Hopitaux Universitaires de Marseille.
FIR - Greillier, Laurent
IR  - Greillier L
IRAD- APHM Hopitaux Universitaires de Marseille.
FIR - Tomasini, Pascale
IR  - Tomasini P
IRAD- APHM Hopitaux Universitaires de Marseille.
FIR - Barlesi, Fabrice
IR  - Barlesi F
IRAD- APHM Hopitaux Universitaires de Marseille.
FIR - Mascaux, Celine
IR  - Mascaux C
IRAD- APHM Hopitaux Universitaires de Marseille.
FIR - Jourdan, Sandrine
IR  - Jourdan S
IRAD- APHM Hopitaux Universitaires de Marseille.
FIR - Roger, Aurelie
IR  - Roger A
IRAD- APHM Hopitaux Universitaires de Marseille.
FIR - Biemar, Julie
IR  - Biemar J
IRAD- APHM Hopitaux Universitaires de Marseille.
FIR - Randriamampionona, Rondro
IR  - Randriamampionona R
IRAD- APHM Hopitaux Universitaires de Marseille.
FIR - Chabot, Francois
IR  - Chabot F
IRAD- Hopital Universitaire de Nancy.
FIR - Tiotiu, Angelica
IR  - Tiotiu A
IRAD- Hopital Universitaire de Nancy.
FIR - Clement-Duchene, Christelle
IR  - Clement-Duchene C
IRAD- Hopital Universitaire de Nancy.
FIR - Vignaud, Jean-Michel
IR  - Vignaud JM
IRAD- Hopital Universitaire de Nancy.
FIR - Lacomme, Stephanie
IR  - Lacomme S
IRAD- Hopital Universitaire de Nancy.
FIR - Lomazzi, Sandra
IR  - Lomazzi S
IRAD- Hopital Universitaire de Nancy.
FIR - Laurent, Carine
IR  - Laurent C
IRAD- Institut de Cancerologie de Lorraine.
FIR - Bulsei, Xavier
IR  - Bulsei X
IRAD- Institut de Cancerologie de Lorraine.
FIR - Bischoff, Laura
IR  - Bischoff L
IRAD- Institut de Cancerologie de Lorraine.
FIR - Rakotonirina, Raymond
IR  - Rakotonirina R
IRAD- Institut de Cancerologie de Lorraine.
FIR - Layouni, Mehdi
IR  - Layouni M
IRAD- Institut de Cancerologie de Lorraine.
FIR - Deslee, Gaetan
IR  - Deslee G
IRAD- Hopital Universitaire de Reims.
FIR - Mal, Herve
IR  - Mal H
IRAD- Hopital Bichat.
FIR - Kessler, Romain
IR  - Kessler R
IRAD- Hopital Universitaire de Strasbourg.
FIR - Vergnon, Jean-Michel
IR  - Vergnon JM
IRAD- Hopital Universitaire de St Etienne.
FIR - Pelissier, Isabelle
IR  - Pelissier I
IRAD- Hopital Universitaire de St Etienne.
FIR - Cuvelier, Antoine
IR  - Cuvelier A
IRAD- Hopital Universitaire de Rouen.
FIR - Bourdin, Arnaud
IR  - Bourdin A
IRAD- Hopital Universitaire de Montpellier.
FIR - Jounieaux, Vincent
IR  - Jounieaux V
IRAD- Hopital Universitaire d'Amiens.
FIR - Roche, Nicolas
IR  - Roche N
IRAD- Hopital Cochin.
FIR - Jouneau, Stephane
IR  - Jouneau S
IRAD- Hopital Universitaire de Rennes.
FIR - Bonniaud, Philippe
IR  - Bonniaud P
IRAD- Hopital Universitaire de Dijon.
FIR - Scherpereel, Arnaud
IR  - Scherpereel A
IRAD- Hopital Universitaire de Lille.
FIR - Mornex, Jean Francois
IR  - Mornex JF
IRAD- Hopital Universitaire de Lyon.
FIR - Steenhouwer, Francois
IR  - Steenhouwer F
IRAD- Hopital de Roubaix.
FIR - Leroy, Sylvie
IR  - Leroy S
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Marquette, Charles Hugo
IR  - Marquette CH
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Benzaquen, Jonathan
IR  - Benzaquen J
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Mazzette, Andrea
IR  - Mazzette A
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Padovani, Bernard
IR  - Padovani B
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Hofman, Paul
IR  - Hofman P
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Ilie, Marius
IR  - Ilie M
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Hofman, Veronique
IR  - Hofman V
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Fayada, Julien
IR  - Fayada J
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Long-Mira, Elodie
IR  - Long-Mira E
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Lassalle, Sandra
IR  - Lassalle S
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Pradelli, Johanna
IR  - Pradelli J
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Martinez, Estelle
IR  - Martinez E
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Habault, Marine
IR  - Habault M
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Bonnard, Melanie
IR  - Bonnard M
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Moutarde, Julie
IR  - Moutarde J
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Yatimi, Rachida
IR  - Yatimi R
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Labsi, Hakima
IR  - Labsi H
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Gazoppi, Loic
IR  - Gazoppi L
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Lpce, Tumorotheque
IR  - Lpce T
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Griffonnet, Jennifer
IR  - Griffonnet J
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Fontaine, Maureen
IR  - Fontaine M
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Guillemart, Ariane
IR  - Guillemart A
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Butori, Catherine
IR  - Butori C
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Selva, Eric
IR  - Selva E
IRAD- Centre Hospitalier Universitaire de Nice.
FIR - Poudenx, Michel
IR  - Poudenx M
IRAD- Centre Antoine Lacassagne de Nice.
FIR - Otto, Josiane
IR  - Otto J
IRAD- Centre Antoine Lacassagne de Nice.
FIR - Hebert, Christophe
IR  - Hebert C
IRAD- Centre Antoine Lacassagne de Nice.
FIR - Botchiellini, Delphine
IR  - Botchiellini D
IRAD- Centre Antoine Lacassagne de Nice.
FIR - Boudouf, Soukaina
IR  - Boudouf S
IRAD- Centre Antoine Lacassagne de Nice.
FIR - Menier, Margaux
IR  - Menier M
IRAD- Centre Antoine Lacassagne de Nice.
FIR - Occeli, Estelle
IR  - Occeli E
IRAD- Centre Antoine Lacassagne de Nice.
FIR - Bellentani, Sophie
IR  - Bellentani S
IRAD- Centre Antoine Lacassagne de Nice.
FIR - Pion, Carine
IR  - Pion C
IRAD- Centre Antoine Lacassagne de Nice.
FIR - Fournier, Elodie
IR  - Fournier E
IRAD- Centre Antoine Lacassagne de Nice.
FIR - Thariat, Juliette
IR  - Thariat J
IRAD- Centre Francois BACLESSE a Caen.
FIR - Gervais, Radj
IR  - Gervais R
IRAD- Centre Francois BACLESSE a Caen.
FIR - Hamond, Karim
IR  - Hamond K
IRAD- Centre Francois BACLESSE a Caen.
FIR - Marchand-Adam, Sylvain
IR  - Marchand-Adam S
IRAD- Hopital Universitaire de Tours.
FIR - Plantier, Laurent
IR  - Plantier L
IRAD- Hopital Universitaire de Tours.
FIR - Fajolle, Gaelle
IR  - Fajolle G
IRAD- Hopital Universitaire de Tours.
FIR - Rayez, Melanie
IR  - Rayez M
IRAD- Hopital Universitaire de Tours.
FIR - Cadranel, Jacques
IR  - Cadranel J
IRAD- Hopital Tenon.
FIR - Fallet, Vincent
IR  - Fallet V
IRAD- Hopital Tenon.
FIR - Wislez, Marie
IR  - Wislez M
IRAD- Hopital Tenon.
FIR - Antoine, Martine
IR  - Antoine M
IRAD- Hopital Tenon.
FIR - Cote, Jean-Francois
IR  - Cote JF
IRAD- Hopital Tenon.
FIR - Chaabane, Nouha
IR  - Chaabane N
IRAD- Hopital Tenon.
FIR - Ruppert, Anne Marie
IR  - Ruppert AM
IRAD- Hopital Tenon.
FIR - Bertrand, Eliane
IR  - Bertrand E
IRAD- Hopital Tenon.
FIR - Rodenas, Anita
IR  - Rodenas A
IRAD- Hopital Tenon.
FIR - Pontdeme, Gwenaelle
IR  - Pontdeme G
IRAD- Hopital Tenon.
FIR - Mathiot, Nathalie
IR  - Mathiot N
IRAD- Hopital Tenon.
FIR - Ribert, Tamazouzt
IR  - Ribert T
IRAD- Hopital Tenon.
FIR - Mazieres, Julien
IR  - Mazieres J
IRAD- Hopital Universitaire de Toulouse.
FIR - Guibert, Nicolas
IR  - Guibert N
IRAD- Hopital Universitaire de Toulouse.
FIR - Rouviere, Damien
IR  - Rouviere D
IRAD- Hopital Universitaire de Toulouse.
FIR - Bousquet, Emilie
IR  - Bousquet E
IRAD- Hopital Universitaire de Toulouse.
FIR - Bigay-Game, Laurence
IR  - Bigay-Game L
IRAD- Hopital Universitaire de Toulouse.
FIR - Hermant, Christophe
IR  - Hermant C
IRAD- Hopital Universitaire de Toulouse.
FIR - Plat, Gavin
IR  - Plat G
IRAD- Hopital Universitaire de Toulouse.
FIR - Rouquette, Isabelle
IR  - Rouquette I
IRAD- Hopital Universitaire de Toulouse.
FIR - Evrard, Solene
IR  - Evrard S
IRAD- Hopital Universitaire de Toulouse.
FIR - Gouin, Sandrine
IR  - Gouin S
IRAD- Hopital Universitaire de Toulouse.
FIR - Clermont, Estelle Taranchon
IR  - Clermont ET
IRAD- Hopital Universitaire de Toulouse.
FIR - Dormoy, Inge
IR  - Dormoy I
IRAD- Hopital Universitaire de Toulouse.
FIR - Coulomb, Christelle
IR  - Coulomb C
IRAD- Hopital Universitaire de Toulouse.
FIR - Pradine, Anne
IR  - Pradine A
IRAD- Hopital Universitaire de Toulouse.
FIR - Lambert, Veronique
IR  - Lambert V
IRAD- Hopital Universitaire de Toulouse.
FIR - Laborde, Lilian
IR  - Laborde L
IRAD- Institut Paoli-Calmettes de Marseille.
FIR - Castelnau, Olivier
IR  - Castelnau O
IRAD- Institut Tzanck de St Laurent du Var.
EDAT- 2021/02/06 06:00
MHDA- 2021/05/25 06:00
CRDT- 2021/02/05 20:11
PHST- 2020/09/30 00:00 [received]
PHST- 2021/01/18 00:00 [revised]
PHST- 2021/01/22 00:00 [accepted]
PHST- 2021/02/06 06:00 [pubmed]
PHST- 2021/05/25 06:00 [medline]
PHST- 2021/02/05 20:11 [entrez]
AID - S1556-0864(21)01664-6 [pii]
AID - 10.1016/j.jtho.2021.01.1617 [doi]
PST - ppublish
SO  - J Thorac Oncol. 2021 May;16(5):807-816. doi: 10.1016/j.jtho.2021.01.1617. Epub 
      2021 Feb 3.