PMID- 33545508
OWN - NLM
STAT- MEDLINE
DCOM- 20220124
LR  - 20240226
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 226
IP  - 2
DP  - 2021 Mar
TI  - Immune induction by adjuvanted Leishmania donovani vaccines against the visceral 
      leishmaniasis in BALB/c mice.
PG  - 152057
LID - S0171-2985(21)00005-X [pii]
LID - 10.1016/j.imbio.2021.152057 [doi]
AB  - Visceral leishmaniasis (VL) is a neglected tropical disease caused by Leishmania 
      donovani or Leishmania infantum. Currently, the patients are treated with 
      chemotherapeutic drugs; however, their toxicity limits their use. It would be 
      desirable to develop a vaccine against this infection. In this study, we assessed 
      the efficacy of different vaccine formulations at variable time points. 
      Heat-killed (HK) antigen of Leishmania donovani was adjuvanted with two adjuvants 
      (AddaVax and Montanide ISA 201) and three immunizations at a gap of 2 weeks (wk) 
      were given to BALB/c mice. After 2 weeks of the last booster, mice were given 
      challenge infection and sacrificed before challenge and after 4wk, 8wk, and 12 wk 
      post-challenge. Significant protective immunity was observed in all the immunized 
      animals and it was indicated by the notable rise in delayed-type hypersensitivity 
      (DTH) response, remarkably declined parasite burden, a significant increase in 
      the levels of interferon-gamma (IFN-gamma), interleukin-12, interleukin-17 (Th1 
      cytokines), and IgG2a in contrast to infected control mice. Montanide ISA 201 
      with HK antigen provided maximum protection followed by AddaVax with HK and then 
      HK alone. These findings elaborate on the importance of the tested adjuvants in 
      the vaccine formulations against murine visceral leishmaniasis.
CI  - Copyright (c) 2021 Elsevier GmbH. All rights reserved.
FAU - Goyal, Deepak Kumar
AU  - Goyal DK
AD  - Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh 
      160014, India.
FAU - Keshav, Poonam
AU  - Keshav P
AD  - Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh 
      160014, India.
FAU - Kaur, Sukhbir
AU  - Kaur S
AD  - Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh 
      160014, India. Electronic address: puzoology@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210123
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Addavax)
RN  - 0 (Adjuvants, Vaccine)
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Leishmaniasis Vaccines)
RN  - 0 (Oleic Acids)
RN  - 0 (Polysorbates)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (montanide ISA-201)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 3OWL53L36A (Mannitol)
RN  - 7QWM220FJH (Squalene)
SB  - IM
MH  - Adjuvants, Vaccine/*administration & dosage
MH  - Animals
MH  - Antibodies, Protozoan/blood
MH  - Antigens, Protozoan/*administration & dosage
MH  - Cytokines/blood
MH  - Female
MH  - Hypersensitivity, Delayed/immunology
MH  - Immunoglobulin G/blood
MH  - *Leishmania donovani
MH  - Leishmaniasis Vaccines/*administration & dosage
MH  - Leishmaniasis, Visceral/*immunology/parasitology/*prevention & control
MH  - Male
MH  - Mannitol/administration & dosage/*analogs & derivatives
MH  - Mice, Inbred BALB C
MH  - Nitric Oxide/immunology
MH  - Oleic Acids/*administration & dosage
MH  - Polysorbates/*administration & dosage
MH  - Reactive Oxygen Species/immunology
MH  - Spleen/cytology/immunology
MH  - Squalene/*administration & dosage
MH  - Mice
OTO - NOTNLM
OT  - AddaVax
OT  - Adjuvant
OT  - Heat-killed antigen
OT  - Montanide ISA 201
OT  - Vaccine
OT  - Visceral leishmaniasis
EDAT- 2021/02/06 06:00
MHDA- 2022/01/27 06:00
CRDT- 2021/02/05 20:13
PHST- 2020/07/21 00:00 [received]
PHST- 2020/12/09 00:00 [revised]
PHST- 2021/01/11 00:00 [accepted]
PHST- 2021/02/06 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2021/02/05 20:13 [entrez]
AID - S0171-2985(21)00005-X [pii]
AID - 10.1016/j.imbio.2021.152057 [doi]
PST - ppublish
SO  - Immunobiology. 2021 Mar;226(2):152057. doi: 10.1016/j.imbio.2021.152057. Epub 
      2021 Jan 23.