PMID- 33545549
OWN - NLM
STAT- MEDLINE
DCOM- 20210511
LR  - 20240226
IS  - 1618-095X (Electronic)
IS  - 0944-7113 (Linking)
VI  - 83
DP  - 2021 Mar
TI  - Amelioration of hyperglycaemia and hyperlipidaemia by adjusting the interplay 
      between gut microbiota and bile acid metabolism: Radix Scutellariae as a case.
PG  - 153477
LID - S0944-7113(21)00019-2 [pii]
LID - 10.1016/j.phymed.2021.153477 [doi]
AB  - BACKGROUND: Our previous clinical research showed that the interaction between 
      gut microbiota and bile acids (BAs) in patients with type 2 diabetes mellitus 
      (T2DM) changed significantly. We hypothesized that T2DM could be improved by 
      adjusting this interaction mediated by farnesoid X receptor (FXR). T2DM belongs 
      to the category of "xiaoke" in traditional Chinese medicine. Radix scutellariae 
      has the effects of clearing away heat and eliminating dampness, curing jaundice 
      and quenching thirst and is widely used alone or in combination with other 
      medicines for the treatment of T2DM in China and throughout Asia. Additionally, 
      the interaction between Radix scutellariae and gut microbiota may influence its 
      efficacy in the treatment of T2DM. PURPOSE: This study chose Radix scutellariae 
      to validate that T2DM could improve by adjusting the interaction between gut 
      microbiota and bile acid metabolism. STUDY DESIGN AND METHODS: Radix scutellariae 
      water extract (WESB) was administered to a T2DM rat model established by a 
      high-fat diet combined with streptozotocin. The body weight and blood glucose and 
      insulin levels were measured. The levels of serum lipids, creatinine, uric acid, 
      albumin and total bile acid were also detected. Changes in the pathology and 
      histology of the pancreas, liver and kidney were observed by haematoxylin-eosin 
      staining. The 16S rRNAs of gut microbiota were sequenced, and the faecal and 
      serum BAs were determined by liquid chromatography tandem mass spectrometry. The 
      expression levels of BA metabolism-associated proteins in the liver and intestine 
      were evaluated by immunoblot analysis. RESULTS: The results showed that WESB 
      improved hyperglycaemia, hyperlipaemia, and liver and kidney damage in T2DM rats. 
      In addition, the abundances of key gut microbiota and the concentrations of 
      certain secondary BAs in faeces and serum were restored. Moreover, there was a 
      significant correlation between the restored gut microbiota and BAs, which might 
      be related to the activation of liver cholesterol 7alpha-hydroxylase (CYP7A1) and the 
      inhibition of FXR expression in the intestine rather than the liver. CONCLUSIONS: 
      This study provided new ideas for the prevention or treatment of clinical 
      diabetes and its complications by adjusting the interaction between gut 
      microbiota and bile acid metabolism.
CI  - Copyright (c) 2021. Published by Elsevier GmbH.
FAU - Zhao, Lijuan
AU  - Zhao L
AD  - School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 
      200240, China; Department of Clinical Pharmacy, the First Affiliated Hospital, 
      Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, 
      China.
FAU - Ma, Ping
AU  - Ma P
AD  - School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 
      200240, China.
FAU - Peng, Ying
AU  - Peng Y
AD  - School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 
      200240, China.
FAU - Wang, Mengyue
AU  - Wang M
AD  - School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 
      200240, China.
FAU - Peng, Chongsheng
AU  - Peng C
AD  - School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 
      200240, China.
FAU - Zhang, Yulong
AU  - Zhang Y
AD  - School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 
      200240, China.
FAU - Li, Xiaobo
AU  - Li X
AD  - School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 
      200240, China. Electronic address: xbli@sjtu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210121
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (Bile Acids and Salts)
RN  - 0 (Drugs, Chinese Herbal)
RN  - EC 1.14.14.23 (CYP7A1 protein, rat)
RN  - EC 1.14.14.23 (Cholesterol 7-alpha-Hydroxylase)
SB  - IM
MH  - Animals
MH  - Bile Acids and Salts/*metabolism
MH  - Cholesterol 7-alpha-Hydroxylase/metabolism
MH  - Diabetes Mellitus, Experimental/drug therapy/metabolism
MH  - Diabetes Mellitus, Type 2/drug therapy/metabolism
MH  - Drugs, Chinese Herbal/chemistry/pharmacology
MH  - Gastrointestinal Microbiome/*drug effects/physiology
MH  - Hyperglycemia/*drug therapy/metabolism/microbiology
MH  - Hyperlipidemias/*drug therapy/metabolism/microbiology
MH  - Intestines/drug effects
MH  - Lipid Metabolism/drug effects
MH  - Liver/drug effects/metabolism
MH  - Male
MH  - Rats, Sprague-Dawley
MH  - Scutellaria baicalensis/*chemistry
MH  - Rats
OTO - NOTNLM
OT  - Radix scutellariae
OT  - bile acid
OT  - farnesoid X receptor
OT  - gut microbiota
OT  - type 2 diabetes mellitus
EDAT- 2021/02/06 06:00
MHDA- 2021/05/12 06:00
CRDT- 2021/02/05 20:13
PHST- 2020/06/02 00:00 [received]
PHST- 2020/11/06 00:00 [revised]
PHST- 2021/01/18 00:00 [accepted]
PHST- 2021/02/06 06:00 [pubmed]
PHST- 2021/05/12 06:00 [medline]
PHST- 2021/02/05 20:13 [entrez]
AID - S0944-7113(21)00019-2 [pii]
AID - 10.1016/j.phymed.2021.153477 [doi]
PST - ppublish
SO  - Phytomedicine. 2021 Mar;83:153477. doi: 10.1016/j.phymed.2021.153477. Epub 2021 
      Jan 21.