PMID- 33545791
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 2224-5839 (Electronic)
IS  - 2224-5820 (Linking)
VI  - 10
IP  - 1
DP  - 2021 Jan
TI  - Effects of Salvia miltiorrhiza injection on apoptosis of Schwann cells induced by 
      hydrogen peroxide.
PG  - 625-632
LID - 10.21037/apm-20-2580 [doi]
AB  - BACKGROUND: Salvia miltiorrhiza is a traditional Chinese medicine with remarkable 
      antioxidant, antibacterial, and anticoagulant properties. In the present study, 
      we investigated the effects of Salvia miltiorrhiza injection in protecting 
      Schwann cells (SCs) from hydrogen peroxide (H2O2)-induced cell apoptosis. 
      METHODS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay 
      and immunofluorescence staining were used to detect the establishment of the SC 
      apoptosis model induced by H2O2. The effect of Salvia miltiorrhiza injection on 
      injured cell morphology was observed, and the effect on cell apoptosis was 
      determined by Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit 
      and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assay. Western blotting analysis was used to detect the effect of Salvia 
      miltiorrhiza injection on apoptosis-related protein expression. RESULTS: The 
      results of the MTT assay showed that cell activity significantly decreased after 
      treatment with 1 mM H2O2, but different concentrations of Salvia miltiorrhiza 
      injection could improve cell activity at different degrees. The number of cells 
      increased significantly after treatment with Salvia miltiorrhiza injection. 
      Annexin V-FITC/PI double staining and TUNEL results revealed that Salvia 
      miltiorrhiza injection could significantly reduce apoptosis induced by H2O2. 
      Western blotting analysis showed that the expression of Bcl-2 was significantly 
      upregulated, while the expression level of Bax was significantly downregulated. 
      CONCLUSIONS: Salvia miltiorrhiza injection can protect SCs from H2O2-induced cell 
      apoptosis, and has potential therapeutic effects in neurological disease.
FAU - Zhong, Jingfei
AU  - Zhong J
AD  - Nantong University Xinglin College, Nantong, China.
FAU - Zhou, Jiaming
AU  - Zhou J
AD  - Nantong University Xinglin College, Nantong, China.
FAU - Sun, Hui
AU  - Sun H
AD  - Nantong University Xinglin College, Nantong, China.
FAU - Wu, Yinping
AU  - Wu Y
AD  - Nantong University Xinglin College, Nantong, China.
FAU - Wu, Ye
AU  - Wu Y
AD  - Nantong University Xinglin College, Nantong, China.
FAU - Li, Meiyuan
AU  - Li M
AD  - Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of 
      Education, Co-Innovatioin Center of Neuroregeneration, Nantong University, 
      Nantong, China. Email: limeiyuan1986@ntu.edu.cn.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Palliat Med
JT  - Annals of palliative medicine
JID - 101585484
RN  - 0 (Drugs, Chinese Herbal)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Apoptosis
MH  - *Drugs, Chinese Herbal
MH  - Hydrogen Peroxide
MH  - *Salvia miltiorrhiza
MH  - Schwann Cells
OTO - NOTNLM
OT  - Salvia miltiorrhiza injection
OT  - Schwann cells (SCs)
OT  - cell apoptosis
OT  - hydrogen peroxide (H2O2)
EDAT- 2021/02/07 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/02/06 01:00
PHST- 2020/11/06 00:00 [received]
PHST- 2021/01/25 00:00 [accepted]
PHST- 2021/02/06 01:00 [entrez]
PHST- 2021/02/07 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - 10.21037/apm-20-2580 [doi]
PST - ppublish
SO  - Ann Palliat Med. 2021 Jan;10(1):625-632. doi: 10.21037/apm-20-2580.