PMID- 33545850
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 120
DP  - 2021 Jan
TI  - An injectable and antibacterial calcium phosphate scaffold inhibiting 
      Staphylococcus aureus and supporting stem cells for bone regeneration.
PG  - 111688
LID - S0928-4931(20)33607-9 [pii]
LID - 10.1016/j.msec.2020.111688 [doi]
AB  - Staphylococcus aureus (S. aureus) is the major pathogen for osteomyelitis, which 
      can lead to bone necrosis and destruction. There has been no report on 
      antibacterial calcium phosphate cement (CPC) against S. aureus. The aims of this 
      study were to: (1) develop novel antibacterial CPC-chitosan-alginate microbead 
      scaffold; (2) investigate mechanical and antibacterial properties of 
      CPC-chitosan-penicillin-alginate scaffold; (3) evaluate the encapsulation and 
      delivery of human umbilical cord mesenchymal stem cells (hUCMSCs). Flexural 
      strength, elastic modulus and work-of-fracture of the 
      CPC-chitosan-penicillin-alginate microbeads scaffold and CPC-chitosan scaffold 
      were evaluated. Penicillin release profile and antibacterial effects on S. aureus 
      were determined. The hUCMSC delivery and release from penicillin-alginate 
      microbeads were investigated. Injectable CPC-chitosan-penicillin-alginate 
      microbeads scaffold was developed for the first time. 
      CPC-chitosan-penicillin-alginate microbeads scaffold had a flexural strength of 
      3.16 +/- 0.55 MPa, matching that of cancellous bone. With sustained penicillin 
      release, the new scaffold had strong antibacterial effects on S. aureus, with an 
      inhibition zone diameter of 32.2 +/- 2.5 mm, greater than that of penicillin disk 
      control (15.1 +/- 2.0 mm) (p < 0.05). Furthermore, this injectable and 
      antibacterial scaffold had no toxic effects, yielding excellent hUCMSC viability, 
      which was similar to that of CPC control without antibacterial activity 
      (p > 0.05). CPC-chitosan-penicillin-microbeads scaffold had injectability, good 
      strength, strong antibacterial effects, and good biocompatibility to support stem 
      cell viability for osteogenesis. CPC-chitosan-penicillin-microbeads scaffold is 
      promising for dental, craniofacial and orthopedic applications to combat 
      infections and promote bone regeneration.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Wu, Shizhou
AU  - Wu S
AD  - Department of Orthopedic Surgery, West China Hospital, Sichuan University, 
      Chengdu, Sichuan 610041, China; Biomaterials & Tissue Engineering Division, 
      Department of Advanced Oral Sciences and Therapeutics, University of Maryland 
      Dental School, Baltimore, MD 21201, USA.
FAU - Lei, Lei
AU  - Lei L
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan 610041, China.
FAU - Bao, Chongyun
AU  - Bao C
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan 610041, China.
FAU - Liu, Jin
AU  - Liu J
AD  - Biomaterials & Tissue Engineering Division, Department of Advanced Oral Sciences 
      and Therapeutics, University of Maryland Dental School, Baltimore, MD 21201, USA; 
      Key Laboratory of Shannxi for Craniofacial Precision Medicine Research, Clinical 
      Research Center of Shannxi for Dental and Maxillofacial Diseases, College of 
      Stomatology, Xi'an Jiaotong University, Shannxi 710004, China.
FAU - Weir, Michael D
AU  - Weir MD
AD  - Biomaterials & Tissue Engineering Division, Department of Advanced Oral Sciences 
      and Therapeutics, University of Maryland Dental School, Baltimore, MD 21201, USA.
FAU - Ren, Ke
AU  - Ren K
AD  - Department of Neural and Pain Sciences, School of Dentistry, Program in 
      Neuroscience, University of Maryland, Baltimore, MD 21201, USA.
FAU - Schneider, Abraham
AU  - Schneider A
AD  - Department of Oncology and Diagnostic Sciences, University of Maryland School of 
      Dentistry, Baltimore, USA; Marlene and Stewart Greenebaum Cancer Center, 
      University of Maryland School of Medicine, Baltimore, MD 21201, USA.
FAU - Oates, Thomas W
AU  - Oates TW
AD  - Biomaterials & Tissue Engineering Division, Department of Advanced Oral Sciences 
      and Therapeutics, University of Maryland Dental School, Baltimore, MD 21201, USA.
FAU - Liu, Jun
AU  - Liu J
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan 610041, China. Electronic address: junliu@scu.edu.cn.
FAU - Xu, Hockin H K
AU  - Xu HHK
AD  - Biomaterials & Tissue Engineering Division, Department of Advanced Oral Sciences 
      and Therapeutics, University of Maryland Dental School, Baltimore, MD 21201, USA; 
      Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of 
      Medicine, Baltimore, MD 21201, USA; Center for Stem Cell Biology and Regenerative 
      Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA. 
      Electronic address: hxu@umaryland.edu.
LA  - eng
PT  - Journal Article
DEP - 20201104
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bone Cements)
RN  - 0 (Calcium Phosphates)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bone Cements/pharmacology
MH  - Bone Regeneration
MH  - Calcium Phosphates/pharmacology
MH  - Cell Differentiation
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - Osteogenesis
MH  - *Staphylococcus aureus
MH  - Stem Cells
MH  - Tissue Engineering
MH  - Tissue Scaffolds
OTO - NOTNLM
OT  - Antibacterial
OT  - Calcium phosphate scaffold
OT  - Injectable
OT  - Penicillin
OT  - Staphylococcus aureus
OT  - Stem cells
EDAT- 2021/02/07 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/02/06 01:00
PHST- 2020/09/07 00:00 [received]
PHST- 2020/10/26 00:00 [revised]
PHST- 2020/10/27 00:00 [accepted]
PHST- 2021/02/06 01:00 [entrez]
PHST- 2021/02/07 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - S0928-4931(20)33607-9 [pii]
AID - 10.1016/j.msec.2020.111688 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111688. doi: 
      10.1016/j.msec.2020.111688. Epub 2020 Nov 4.