PMID- 33546151
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231110
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 11
IP  - 2
DP  - 2021 Feb 3
TI  - Green Synthesis of Silver Nanoparticles Using Annona muricata Extract as an 
      Inducer of Apoptosis in Cancer Cells and Inhibitor for NLRP3 Inflammasome via 
      Enhanced Autophagy.
LID - 10.3390/nano11020384 [doi]
LID - 384
AB  - Annona muricata is one of the most important traditional medicinal plants which 
      contains numerous chemicals that exhibit various pharmacological properties. In 
      this study, silver nanoparticles were prepared using A. muricata peel extract as 
      a reducing agent and the effect was enhanced through A. muricata like 
      pharmaceutical activity. AgNPs formation was confirmed by color changes, 
      UV-visible spectroscopy, SEM, DLS, and XRD. The anti-proliferative activity of 
      AgNPs against THP-1, AMJ-13, and HBL cell lines was studied. Apoptotic markers 
      were tested using AO/EtBr staining assay, cell cycle phases using flowcytometry, 
      and the expression of P53. Autophagy takes an essential part in controlling 
      inflammasome activation by primary bone marrow-derived macrophages (BMDMs). We 
      report novel functions for AgNPs-affected autophagy, represented by the control 
      of the release of IL-1beta, caspase-1, adaptor protein apoptosis-associated 
      speck-like protein containing a CARD (ASC), and NLRP3 in BMDMs following 
      treatment with LPS+ATP. The current study revealed that the AgNPs inhibited THP-1 
      and AMJ-13 cell proliferation. Meanwhile, the AgNPs significantly increased 
      autophagy and reduced IL-1b and NLRP3 levels in both in vivo and in vitro models. 
      The secretion of IL-1beta was reduced whereas the degradation of NLRP3 inflammasome 
      was enhanced. These findings propose that AgNPs apply an anti-proliferative 
      activity against THP-1 and AMJ-13 cells through the stimulation of apoptosis via 
      mitochondrial damage and induction of p53 protein pathway. In addition, 
      AgNP-induced autophagy reduced the levels of IL-1beta and NLRP3 inflammasome 
      activation. This indicated that the AgNPs augment autophagy controlled by the 
      IL-1beta pathway via two different novel mechanisms. The first one is regulating 
      activation of the IL-1 beta, caspae-1, and ASC, while the second is NLRP3 targeting 
      for lysosomal degradation. Overall, this study suggests that AgNPs could be a 
      potent therapy for various types of cancer and an alternative treatment for 
      preventing inflammation via enhancing autophagy.
FAU - Jabir, Majid S
AU  - Jabir MS
AD  - Department of Applied Sciences, University of Technology, Baghdad 10066, Iraq.
FAU - Saleh, Yasmin M
AU  - Saleh YM
AD  - College of Education, Mustansiriyah University, Baghdad 10052, Iraq.
FAU - Sulaiman, Ghassan M
AU  - Sulaiman GM
AUID- ORCID: 0000-0001-6388-3849
AD  - Department of Applied Sciences, University of Technology, Baghdad 10066, Iraq.
FAU - Yaseen, Nahi Y
AU  - Yaseen NY
AD  - Iraqi Center for Cancer and Medical Genetics Research, Mustansiriyah University, 
      Baghdad 10052, Iraq.
FAU - Sahib, Usama I
AU  - Sahib UI
AD  - Department of Applied Sciences, University of Technology, Baghdad 10066, Iraq.
FAU - Dewir, Yaser Hassan
AU  - Dewir YH
AUID- ORCID: 0000-0002-5174-8137
AD  - Plant Production Department, P.O. Box 2460, College of Food and Agriculture 
      Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
AD  - Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
FAU - Alwahibi, Mona S
AU  - Alwahibi MS
AUID- ORCID: 0000-0002-3420-1164
AD  - Department of Botany and Microbiology, P.O. Box 22452, College of Science, King 
      Saud University, Riyadh 11495, Saudi Arabia.
FAU - Soliman, Dina A
AU  - Soliman DA
AUID- ORCID: 0000-0001-6729-4347
AD  - Department of Botany and Microbiology, P.O. Box 22452, College of Science, King 
      Saud University, Riyadh 11495, Saudi Arabia.
LA  - eng
GR  - RGP-1435-086/Deanship of Scientific Research at King Saud University/
PT  - Journal Article
DEP - 20210203
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC7913157
OTO - NOTNLM
OT  - Annona muricata
OT  - IL-1- beta
OT  - NLRP3
OT  - anticancer
OT  - apoptosis
OT  - autophagy
OT  - silver nanoparticle
COIS- The authors declare no conflict of interest.
EDAT- 2021/02/07 06:00
MHDA- 2021/02/07 06:01
PMCR- 2021/02/03
CRDT- 2021/02/06 01:02
PHST- 2020/12/25 00:00 [received]
PHST- 2021/01/25 00:00 [revised]
PHST- 2021/01/29 00:00 [accepted]
PHST- 2021/02/06 01:02 [entrez]
PHST- 2021/02/07 06:00 [pubmed]
PHST- 2021/02/07 06:01 [medline]
PHST- 2021/02/03 00:00 [pmc-release]
AID - nano11020384 [pii]
AID - nanomaterials-11-00384 [pii]
AID - 10.3390/nano11020384 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2021 Feb 3;11(2):384. doi: 10.3390/nano11020384.