PMID- 33548622
OWN - NLM
STAT- MEDLINE
DCOM- 20210510
LR  - 20221207
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 545
DP  - 2021 Mar 19
TI  - Effects of transforming growth factor-beta1 on odontoblastic differentiation in 
      dental papilla cells is determined by IPO7 expression level.
PG  - 105-111
LID - S0006-291X(21)00127-3 [pii]
LID - 10.1016/j.bbrc.2021.01.076 [doi]
AB  - Transforming growth factor beta1 (TGF-beta1) is one of the broad-spectrum 
      growth-promoting factors that participate in tooth development. The influence of 
      TGF-beta1 on the odontoblastic differentiation is still controvercy. Mouse primary 
      dental papilla cells (mDPCs) as well as an immortalized mouse dental papilla cell 
      line (mDPC6Ts) were treated with exogenous TGF-beta1 during odontoblastic 
      differentiation. RT-qPCR, Western blot, alizarin red staining and ALP staining 
      were carried out to investigate the influence of TGF-beta1 on odontoblastic 
      differentiation. IPO7, important for SMAD complex translocation was also detected 
      in mDPCs and mDPC6Ts in response to TGF-beta1. After silencing IPO7 by transfection, 
      the translocation process of P-SMAD2 was investigated by nuclear and cytoplasmic 
      extraction as well as co-immunoprecipitation assay. The odontogenic markers, 
      mineralization and IPO7 expression were significantly up-regulated in 
      TGF-beta1-treated mDPCs while down-regulated in mDPC6Ts. The total level of P-SMAD2 
      was not influenced by IPO7 in mDPCs, however, IPO7 could bind to P-SMAD2 and 
      affect the nuclear-cytoplasm-shuttling of P-SMAD2. Our data demonstrated that 
      TGF-beta1 plays opposite roles in odontoblast differentiation in mDPCs and 
      immortalized mouse dental papilla cell line (mDPC6Ts), which is determined by 
      IPO7.
CI  - Copyright (c) 2021. Published by Elsevier Inc.
FAU - Zhang, Yue
AU  - Zhang Y
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key 
      Laboratory of Oral Biomedicine of Ministry of Education, School and Hospital of 
      Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, China. Electronic 
      address: 2018203040020@whu.edu.cn.
FAU - Zhang, Hao
AU  - Zhang H
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key 
      Laboratory of Oral Biomedicine of Ministry of Education, School and Hospital of 
      Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, China. Electronic 
      address: 2012302220058@whu.edu.cn.
FAU - Yuan, Guohua
AU  - Yuan G
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key 
      Laboratory of Oral Biomedicine of Ministry of Education, School and Hospital of 
      Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, China. Electronic 
      address: yuanguohua@whu.edu.cn.
FAU - Yang, Guobin
AU  - Yang G
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key 
      Laboratory of Oral Biomedicine of Ministry of Education, School and Hospital of 
      Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, China. Electronic 
      address: guobin.yang@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210203
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Karyopherins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Smad2 Protein)
RN  - 0 (Smad2 protein, mouse)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Ipo7 protein, mouse)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - Animals
MH  - Cell Differentiation/genetics/physiology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Dental Papilla
MH  - Down-Regulation
MH  - Gene Knockdown Techniques
MH  - Karyopherins/antagonists & inhibitors/genetics/*metabolism
MH  - Mice
MH  - Odontoblasts/*cytology/*metabolism
MH  - Odontogenesis/genetics/physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Smad2 Protein/metabolism
MH  - Transforming Growth Factor beta1/*metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - Importin 7
OT  - Mouse dental papilla cell line
OT  - Mouse dental papilla cells (mDPCs)
OT  - Odontoblastic differentiation
OT  - P-SMAD2
OT  - TGF-beta1
COIS- Declaration of competing interest The authors declared no potential conflicts of 
      interest with respect to this study.
EDAT- 2021/02/07 06:00
MHDA- 2021/05/11 06:00
CRDT- 2021/02/06 20:11
PHST- 2020/12/27 00:00 [received]
PHST- 2021/01/20 00:00 [accepted]
PHST- 2021/02/07 06:00 [pubmed]
PHST- 2021/05/11 06:00 [medline]
PHST- 2021/02/06 20:11 [entrez]
AID - S0006-291X(21)00127-3 [pii]
AID - 10.1016/j.bbrc.2021.01.076 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2021 Mar 19;545:105-111. doi: 
      10.1016/j.bbrc.2021.01.076. Epub 2021 Feb 3.