PMID- 3355003
OWN - NLM
STAT- MEDLINE
DCOM- 19880422
LR  - 20071114
IS  - 0003-0805 (Print)
IS  - 0003-0805 (Linking)
VI  - 137
IP  - 4
DP  - 1988 Apr
TI  - Surfactant-associated proteins in tracheal aspirates of infants with respiratory 
      distress syndrome after surfactant therapy.
PG  - 943-7
AB  - We have developed enzyme-linked immunoassays (ELISAs) that measure major proteins 
      that are associated with pulmonary surfactant. Using these ELISAs, we tested 
      sequential tracheal aspirates from infants severely ill with respiratory distress 
      syndrome (RDS) who had been treated either with exogenous surfactant or with 
      placebo within 8 h of birth. On average, we found low concentrations of 
      surfactant proteins in tracheal aspirates on Day 1 of life, with increases 
      evident by Day 3. The surfactant used in this study (TA surfactant) contains only 
      the low molecular weight (6 kDa) surfactant proteins and not the 35 kDa 
      surfactant protein. As we expected, those who were treated with TA surfactant 
      more frequently had detectable concentrations of low molecular weight surfactant 
      protein on the second day of life when compared with control infants. No 
      differences were evident in the concentrations of surfactant proteins between the 
      2 groups by Day 3, nor were differences evident between the 2 groups evident for 
      35 kDa surfactant protein during the first 3 days of life. Increased low 
      molecular weight surfactant proteins in tracheal aspirates 1 to 2 days after 
      surfactant therapy may occur either because of persistence of exogenous 
      surfactant proteins and/or enhanced surfactant protein production. Comparisons 
      with measurements from other groups of patients with RDS confirm that absence of 
      both surfactant proteins reflects alveolar surfactant deficiency.
FAU - Chida, S
AU  - Chida S
AD  - Department of Pediatrics, Iwate Medical University, Japan.
FAU - Phelps, D S
AU  - Phelps DS
FAU - Cordle, C
AU  - Cordle C
FAU - Soll, R
AU  - Soll R
FAU - Floros, J
AU  - Floros J
FAU - Taeusch, H W
AU  - Taeusch HW
LA  - eng
GR  - HL-27372/HL/NHLBI NIH HHS/United States
GR  - HL-34597/HL/NHLBI NIH HHS/United States
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am Rev Respir Dis
JT  - The American review of respiratory disease
JID - 0370523
RN  - 0 (Proteolipids)
RN  - 0 (Pulmonary Surfactant-Associated Proteins)
RN  - 0 (Pulmonary Surfactants)
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Infant, Newborn
MH  - Molecular Weight
MH  - Osmolar Concentration
MH  - Proteolipids/*metabolism
MH  - Pulmonary Surfactant-Associated Proteins
MH  - Pulmonary Surfactants/*metabolism/*therapeutic use
MH  - Reference Values
MH  - Respiratory Distress Syndrome, Newborn/drug therapy/*metabolism
MH  - Serum Albumin/metabolism
MH  - Suction
MH  - Trachea/*metabolism
EDAT- 1988/04/01 00:00
MHDA- 1988/04/01 00:01
CRDT- 1988/04/01 00:00
PHST- 1988/04/01 00:00 [pubmed]
PHST- 1988/04/01 00:01 [medline]
PHST- 1988/04/01 00:00 [entrez]
AID - 10.1164/ajrccm/137.4.943 [doi]
PST - ppublish
SO  - Am Rev Respir Dis. 1988 Apr;137(4):943-7. doi: 10.1164/ajrccm/137.4.943.