PMID- 33550334 OWN - NLM STAT- MEDLINE DCOM- 20210209 LR - 20230919 IS - 1671-167X (Print) IS - 1671-167X (Linking) VI - 53 IP - 1 DP - 2020 Nov 10 TI - [Clinicopathological analysis of mucosa associated lymphoid tissue lymphoma secondary to Sjogren' s syndrome in salivary gland]. PG - 40-45 AB - OBJECTIVE: To analyze the clinicopathological characteristics of mucosa associated lymphoid tissue (MALT) lymphoma secondary to Sjogren' s syndrome (SS) (SS-MALT lymphoma) in salivary gland and to explore the value of the combined application of histopathological morphology, protein expression and molecular phenotype in pathological diagnosis and prognostic evaluation of SS-MALT lymphoma. METHODS: Sixteen patients with SS-MALT lymphoma were collected from 260 patients who were diagnosed with SS in Peking University School and Hospital of Stomatology from January 1997 to December 2016. Twelve patients with non-MALT lymphoma secondary to SS (non-SS-MALT lymphoma) in salivary gland were selected as controls. The clinical data of the patients were retrospectively reviewed and analyzed. All the patients were followed up until December 20, 2019. Hematoxylin-eosin staining, immunohistochemistry, polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques were used to observe the histologic characteristics and to detect the manifestations of light chain restrictive expression, immunoglobulin (Ig) gene clonal rearrangement, chromosome translocation and gene abnormality, so as to evaluate their values in pathological diagnosis and prognostic evaluation. RESULTS: The malignant transformation rate of SS to MALT lymphoma was about 6.15%, ranged from 3 to 240 months, during which 2 patients died due to high-level deterioration. Microscopically, the acini of the glandular tissue were atrophied and destroyed. The tumor cells dominated by central cell-like lymphocytes grew diffusely, destroying the epithelial islands. All SS-MALT lymphoma cases were positive in CD20 and Pax5. Half of them had the Ki-67 proliferation index of 10% or less, and half greater than 10%. 93.75% cases expressed AE1/AE3 protein, which showed the residual glandular epithelium. All the tumor cells were negative in CD3epsilon, and the plasma cells were detected by CD138 antigen. The light chain restrictive expression of kappa and lambda was 37.5% in SS-MALT lymphoma group. The positive detection rates of immunoglobulin heavy chain (IgH)-FR1, IgH-FR2, IgH-FR3, immunoglobulin kappa chain (IgK)-A, and IgK-B in SS-MALT lymphoma group were 33.3%, 53.3%, 33.3%, 20.0%, and 26.7%, respectively, and 93.3% when together used with IgH and IgK. The positive rates of the MALT1, IGH and BCL6 genes with dual color break-apart probes were 36.4%, 27.3% and 27.3%, and the detection rate of chromosome translocation and gene abnormality by applying the three probes was 72.7%. CONCLUSION: There are no specific histological characteristics and protein phenotypes in the histologic diagnosis of SS-MALT lymphoma in salivary gland. The combined application of histopathological manifestations, immunohistochemistry, PCR and FISH techniques helps the accurate pathologic diagnosis of the disease. Although SS-MALT lymphoma is considered as an indolent lymphoma with a relatively favorable prognosis, the regular return visit and long-term follow-up should be conducted to detect the clues of recurrence and advanced deterioration. FAU - Chi, Y T AU - Chi YT AD - Department of Oral Pathology, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China. AD - Research Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences (2019RU034), Beijing 100081, China. FAU - Zhang, Y P AU - Zhang YP AD - Department of Pathology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. FAU - Zhang, Q L AU - Zhang QL AD - West China School of Medicine, Sichuan University, Chengdu 610041, China. FAU - Liu, C L AU - Liu CL AD - Department of Pathology, Peking University School of Basic Medical Science, Beijing 100191, China. AD - Department of Pathology, Peking University Third Hospital, Beijing 100191, China. FAU - Li, B B AU - Li BB AD - Department of Oral Pathology, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China. AD - Research Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences (2019RU034), Beijing 100081, China. LA - chi PT - Journal Article PL - China TA - Beijing Da Xue Xue Bao Yi Xue Ban JT - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences JID - 101125284 SB - IM MH - Humans MH - In Situ Hybridization, Fluorescence MH - *Lymphoma, B-Cell, Marginal Zone/etiology MH - Neoplasm Recurrence, Local MH - Retrospective Studies MH - Salivary Glands PMC - PMC7867984 OTO - NOTNLM OT - Immunohistochemistry OT - In situ hybridization, fluorescence OT - Mucosa associated lymphoid tissue lymphoma OT - Polymerase chain reaction OT - Sjogren's syndrome EDAT- 2021/02/08 06:00 MHDA- 2021/02/10 06:00 PMCR- 2021/02/18 CRDT- 2021/02/07 20:51 PHST- 2021/02/07 20:51 [entrez] PHST- 2021/02/08 06:00 [pubmed] PHST- 2021/02/10 06:00 [medline] PHST- 2021/02/18 00:00 [pmc-release] AID - bjdxxbyxb-53-1-40 [pii] AID - 10.19723/j.issn.1671-167X.2021.01.007 [doi] PST - ppublish SO - Beijing Da Xue Xue Bao Yi Xue Ban. 2020 Nov 10;53(1):40-45. doi: 10.19723/j.issn.1671-167X.2021.01.007.