PMID- 33551454
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 0013-4686 (Print)
IS  - 0013-4686 (Linking)
VI  - 290
DP  - 2018 Nov 10
TI  - A graphene aptasensor for biomarker detection in human serum.
PG  - 356-363
LID - 10.1016/j.electacta.2018.08.062 [doi]
AB  - This paper presents an affinity graphene nanosensor for detection of biomarkers 
      in undiluted and non-desalted human serum. The affinity nanosensor is a 
      field-effect transistor in which graphene serves as the conducting channel. The 
      graphene surface is sequentially functionalized with a nanolayer of the polymer 
      polyethylene glycol (PEG) and a biomarker-specific aptamer. The aptamer is able 
      to specifically bind with and capture unlabeled biomarkers in serum. A captured 
      biomarker induces a change in the electric conductivity of the graphene, which is 
      measured in a buffer of optimally chosen ionic strength to determine the 
      biomarker concentration. The PEG layer effectively rejects nonspecific adsorption 
      of background molecules in serum while still allowing the aptamer to be readily 
      accessible to serum-borne biomarkers and increases the effective Debye screening 
      length on the graphene surface. Thus, the aptamer-biomarker binding sensitively 
      changes the graphene conductivity, thereby achieving specific and label-free 
      detection of biomarkers with high sensitivity and without the need to dilute or 
      desalt the serum. Experimental results demonstrate that the graphene nanosensor 
      is capable of specifically capturing human immunoglobulin E (IgE), used as a 
      representative biomarker, in human serum in the concentration range of 50 pM-250 
      nM, with a resolution of 14.5 pM and a limit of detection of 47 pM.
FAU - Wang, Xuejun
AU  - Wang X
AD  - Department of Mechanical Engineering, East China University of Science and 
      Technology, Shanghai, 200237, China.
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027, 
      USA.
FAU - Zhu, Yibo
AU  - Zhu Y
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027, 
      USA.
FAU - Olsen, Timothy R
AU  - Olsen TR
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027, 
      USA.
FAU - Sun, Na
AU  - Sun N
AD  - Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 
      Suzhou, Jiangsu, 215123, China.
FAU - Zhang, Wenjun
AU  - Zhang W
AD  - Department of Mechanical Engineering, East China University of Science and 
      Technology, Shanghai, 200237, China.
AD  - Department of Mechanical Engineering, University of Saskatchewan, Saskatoon, S7N 
      5A9, Canada.
FAU - Pei, Renjun
AU  - Pei R
AD  - Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 
      Suzhou, Jiangsu, 215123, China.
FAU - Lin, Qiao
AU  - Lin Q
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027, 
      USA.
LA  - eng
GR  - R33 CA196470/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20180913
PL  - United States
TA  - Electrochim Acta
JT  - Electrochimica acta
JID - 9875994
PMC - PMC7861490
MID - NIHMS993264
OTO - NOTNLM
OT  - Affinity sensing
OT  - Biomarker detection
OT  - Graphene
OT  - Human serum
OT  - Nonspecific binding
EDAT- 2018/11/10 00:00
MHDA- 2018/11/10 00:01
PMCR- 2021/02/04
CRDT- 2021/02/08 05:33
PHST- 2021/02/08 05:33 [entrez]
PHST- 2018/11/10 00:00 [pubmed]
PHST- 2018/11/10 00:01 [medline]
PHST- 2021/02/04 00:00 [pmc-release]
AID - 10.1016/j.electacta.2018.08.062 [doi]
PST - ppublish
SO  - Electrochim Acta. 2018 Nov 10;290:356-363. doi: 10.1016/j.electacta.2018.08.062. 
      Epub 2018 Sep 13.