PMID- 33551645
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220420
IS  - 1179-1322 (Print)
IS  - 1179-1322 (Electronic)
IS  - 1179-1322 (Linking)
VI  - 13
DP  - 2021
TI  - LncRNA GAS5 Suppressed Proliferation and Promoted Apoptosis in Laryngeal Squamous 
      Cell Carcinoma by Targeting MiR-26a-5p and Modifying ULK2.
PG  - 871-887
LID - 10.2147/CMAR.S250778 [doi]
AB  - PURPOSE: Long noncoding RNAs growth arrest-specific 5 (GAS5) exerts important 
      functions in modulating various tumor behaviors. However, the role of lncRNA GAS5 
      in laryngeal squamous cell carcinoma (LSCC) remains unknown. MATERIALS AND 
      METHODS: Cell viability and apoptosis were, respectively, detected by cell 
      counting kit-8 and flow cytometry, DIANA-LncBase V, Starbase, TargetScan and a 
      dual-luciferase reporter gene assay were employed to assess the relationship 
      among GAS5, miR-26a-5p and uncoordinated 51-like kinase 1 (ULK2), and 
      quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and 
      Western blot were performed to detect the expression of autophagy-relative 
      factors. RESULTS: The expression level of GAS5 was frequently decreased in LSCC 
      cell lines, and up-regulated GAS5 inhibited AMC-HN-8 cells viability and induced 
      apoptosis. More importantly, we found that GAS5 activated autophagy, with 
      enhanced autophagy-related proteins after GAS5 overexpression. While 
      down-regulated GAS5 had opposite results in Tu 177 cells, GAS5 was found to act 
      as a microRNA sponge in a pathway to regulate miR-26a-5p and its target gene 
      ULK2. MiR-26a-5p mimics inhibited apoptosis and autophagy, which were reversed by 
      GAS5 and siGAS5 in AMC-HN-8 cells and Tu 177 cells, as well as ULK2 in AMC-HN-8 
      cells. Meanwhile, the concomitant downregulation of ULK2 and miRNA-26a-5p 
      inhibitor decreased the miRNA-26a-5p inhibitor-induced apoptosis and autophagy. 
      CONCLUSION: This is the first report of LncRNA GAS5 acting as a tumor suppressor 
      in LSCC by regulating the miR-26a-5p/ULK2 axis, and it could be a new target for 
      gene therapy in LSCC.
CI  - (c) 2021 Wang et al.
FAU - Wang, Jian
AU  - Wang J
AD  - Department of Head and Neck Surgery, National Cancer Center/Cancer Hospital, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 
      100021, People's Republic of China.
FAU - Zhu, Yiming
AU  - Zhu Y
AD  - Department of Head and Neck Surgery, National Cancer Center/Cancer Hospital, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 
      100021, People's Republic of China.
FAU - Ni, Song
AU  - Ni S
AD  - Department of Head and Neck Surgery, National Cancer Center/Cancer Hospital, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 
      100021, People's Republic of China.
FAU - Liu, Shaoyan
AU  - Liu S
AD  - Department of Head and Neck Surgery, National Cancer Center/Cancer Hospital, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 
      100021, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210129
PL  - New Zealand
TA  - Cancer Manag Res
JT  - Cancer management and research
JID - 101512700
PMC - PMC7856352
OTO - NOTNLM
OT  - apoptosis
OT  - autophagy
OT  - laryngeal squamous cell carcinoma
OT  - long noncoding RNAs growth arrest-specific 5
OT  - miR-26a-5p
COIS- The authors declare no conflicts of interest.
EDAT- 2021/02/09 06:00
MHDA- 2021/02/09 06:01
PMCR- 2021/01/29
CRDT- 2021/02/08 05:34
PHST- 2020/02/21 00:00 [received]
PHST- 2020/07/18 00:00 [accepted]
PHST- 2021/02/08 05:34 [entrez]
PHST- 2021/02/09 06:00 [pubmed]
PHST- 2021/02/09 06:01 [medline]
PHST- 2021/01/29 00:00 [pmc-release]
AID - 250778 [pii]
AID - 10.2147/CMAR.S250778 [doi]
PST - epublish
SO  - Cancer Manag Res. 2021 Jan 29;13:871-887. doi: 10.2147/CMAR.S250778. eCollection 
      2021.