PMID- 33553018
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231110
IS  - 2251-6581 (Print)
IS  - 2251-6581 (Electronic)
IS  - 2251-6581 (Linking)
VI  - 19
IP  - 2
DP  - 2020 Dec
TI  - SNPs in the 3'-untranslated region of SLC30A8 confer risk of type 2 diabetes 
      mellitus in a south-east Iranian population: Evidences from case-control and 
      bioinformatics studies.
PG  - 979-988
LID - 10.1007/s40200-020-00590-5 [doi]
AB  - BACKGROUND: Type 2 diabetes mellitus (T2DM) is a heterogenic disease with 
      increasing incidence. The SLC30A8 gene encodes an islet zinc transporter (ZnT8), 
      and its variants have been associated with glucose and pro-insulin levels. This 
      study was aimed to examine the effects of a missense variant (rs13266634 C/T), 
      and two 3'UTR variants (rs2466294 C/G and rs2466293 T/C) in SLC30A8 gene on T2DM 
      risk in a south-east Iranian population. METHODS: In this experiment, 450 
      patients diagnosed with T2DM and 453 healthy subjects from the same geographic 
      area were enrolled. Genotypes were amplified using the ARMS-PCR method. In silico 
      analyses were performed to determine the effects of the variants on the local 
      structure of mRNA, splicing patterns, and potential miRNA-gene interactions as 
      well. RESULTS: Significant differences were noticed between cases and controls 
      regarding the genotypic and allelic distribution of the studied variants. As 
      regards rs2466293 and rs2466294 variants, enhanced risk of T2DM was found under 
      allelic, dominant, recessive, and codominant models (OR > 1). Besides, different 
      genetic models of rs13266634 were associated with decreased risk of T2DM 
      (OR < 1). Bioinformatics analyses indicated that the rs2466293 variant might 
      influence the binding of some miRNAs, while the G-allele of rs2466294 decreased 
      the stability of SLC30A8-mRNA. CONCLUSIONS: In our population, both SNPs in the 
      3'-untranslated region of SLC30A8 increased the risk of T2DM, while the 
      rs13266634 variant showed a protective association against T2DM susceptibility. 
      Investigating the effects of other variants in this gene or other ZnTs can 
      further indicate such associations in subjects from the same ethnicity.
CI  - (c) Springer Nature Switzerland AG 2020.
FAU - Sargazi, Saman
AU  - Sargazi S
AD  - Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan 
      University of Medical Sciences, Zahedan, Iran. GRID: grid.488433.0. ISNI: 0000 
      0004 0612 8339
FAU - Heidari Nia, Milad
AU  - Heidari Nia M
AD  - Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan 
      University of Medical Sciences, Zahedan, Iran. GRID: grid.488433.0. ISNI: 0000 
      0004 0612 8339
FAU - Sargazi, Fariba Mirani
AU  - Sargazi FM
AD  - Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan 
      University of Medical Sciences, Zahedan, Iran. GRID: grid.488433.0. ISNI: 0000 
      0004 0612 8339
FAU - Sheervalilou, Roghayeh
AU  - Sheervalilou R
AD  - Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan 
      University of Medical Sciences, Zahedan, Iran. GRID: grid.488433.0. ISNI: 0000 
      0004 0612 8339
FAU - Saravani, Ramin
AU  - Saravani R
AD  - Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan 
      University of Medical Sciences, Zahedan, Iran. GRID: grid.488433.0. ISNI: 0000 
      0004 0612 8339
AD  - Department of Clinical Biochemistry, School of Medicine, Zahedan University of 
      Medical Sciences, Zahedan, Iran. GRID: grid.488433.0. ISNI: 0000 0004 0612 8339
FAU - Mirinejad, Shekoufeh
AU  - Mirinejad S
AD  - Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan 
      University of Medical Sciences, Zahedan, Iran. GRID: grid.488433.0. ISNI: 0000 
      0004 0612 8339
LA  - eng
PT  - Journal Article
DEP - 20200721
PL  - Switzerland
TA  - J Diabetes Metab Disord
JT  - Journal of diabetes and metabolic disorders
JID - 101590741
PMC - PMC7843856
OTO - NOTNLM
OT  - Diabetes mellitus
OT  - Gene Polymorphism
OT  - SLC30A8
COIS- Conflict of interestNone declared.
EDAT- 2021/02/09 06:00
MHDA- 2021/02/09 06:01
PMCR- 2021/07/21
CRDT- 2021/02/08 05:44
PHST- 2020/05/08 00:00 [received]
PHST- 2020/06/26 00:00 [revised]
PHST- 2020/07/15 00:00 [accepted]
PHST- 2021/02/08 05:44 [entrez]
PHST- 2021/02/09 06:00 [pubmed]
PHST- 2021/02/09 06:01 [medline]
PHST- 2021/07/21 00:00 [pmc-release]
AID - 590 [pii]
AID - 10.1007/s40200-020-00590-5 [doi]
PST - epublish
SO  - J Diabetes Metab Disord. 2020 Jul 21;19(2):979-988. doi: 
      10.1007/s40200-020-00590-5. eCollection 2020 Dec.