PMID- 33554756
OWN - NLM
STAT- MEDLINE
DCOM- 20220316
LR  - 20220531
IS  - 1531-2267 (Electronic)
IS  - 1094-8341 (Print)
IS  - 1094-8341 (Linking)
VI  - 53
IP  - 4
DP  - 2021 Apr 1
TI  - Shared aspects of mRNA expression associated with oocyte maturation failure in 
      humans and rhesus monkeys indicating compromised oocyte quality.
PG  - 137-149
LID - 10.1152/physiolgenomics.00155.2020 [doi]
AB  - Oocyte maturation failure observed in assisted reproduction technology (ART) 
      cycles can limit the number of quality oocytes obtained and present a pronounced 
      barrier for some patients. The potential exists to use unmatured oocytes for ART 
      through in vitro maturation. Understanding the molecular basis of oocyte 
      maturation failure is pertinent to minimizing this loss of oocytes and 
      considerations of whether such oocytes can be used safely for ART. We identified 
      shared transcriptome abnormalities for rhesus monkey and human failed-to-mature 
      (FTM) oocytes relative to healthy matured MII stage oocytes. We discovered that, 
      although the number of shared affected genes was comparatively small, FTM oocytes 
      in both species shared effects for several pathways and functions, including 
      predicted activation of oxidative phosphorylation (OxPhos) with additional 
      effects on mitochondrial function, lipid metabolism, transcription, nucleotide 
      excision repair, endoplasmic reticulum stress, unfolded protein response, and 
      cell viability. RICTOR emerged as a prominent upstream regulator with predicted 
      inhibition across all analyses. Alterations in KDM5A, MTOR, MTORC1, INSR, CAB39L, 
      and STK11 activities were implicated along with RICTOR in modulating 
      mitochondrial activity and OxPhos. Defects in cell cycle progression were not a 
      prominent feature of FTM oocytes. These results identify a common set of 
      transcriptome abnormalities associated with oocyte maturation failure. While our 
      results do not demonstrate causality, they indicate that fundamental aspects of 
      cellular function are abnormal in FTM oocytes and raise significant concerns 
      about the potential risks of using FTM oocytes for ART.
FAU - Ruebel, Meghan L
AU  - Ruebel ML
AUID- ORCID: 0000-0003-4303-1491
AD  - Department of Animal Science and Reproductive and Developmental Sciences Program, 
      Michigan State University, East Lansing, Michigan.
FAU - Zambelli, Filippo
AU  - Zambelli F
AD  - Clinica EUGIN, Barcelona, Spain.
FAU - Schall, Peter Z
AU  - Schall PZ
AD  - Department of Animal Science and Reproductive and Developmental Sciences Program, 
      Michigan State University, East Lansing, Michigan.
FAU - Barragan, Montserrat
AU  - Barragan M
AD  - Clinica EUGIN, Barcelona, Spain.
FAU - VandeVoort, Catherine A
AU  - VandeVoort CA
AD  - California National Primate Research Center, University of California, Davis, 
      California.
AD  - Department of Obstetrics and Gynecology, University of California, Davis, 
      California.
FAU - Vassena, Rita
AU  - Vassena R
AD  - Clinica EUGIN, Barcelona, Spain.
FAU - Latham, Keith E
AU  - Latham KE
AUID- ORCID: 0000-0003-1206-9059
AD  - Department of Animal Science and Reproductive and Developmental Sciences Program, 
      Michigan State University, East Lansing, Michigan.
LA  - eng
GR  - R24 OD012221/OD/NIH HHS/United States
GR  - T32HD087166/HD/NICHD NIH HHS/United States
GR  - T32 HD087166/HD/NICHD NIH HHS/United States
GR  - P51 OD011107/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20210208
PL  - United States
TA  - Physiol Genomics
JT  - Physiological genomics
JID - 9815683
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *In Vitro Oocyte Maturation Techniques/methods
MH  - Macaca mulatta/genetics
MH  - Mitochondria/metabolism
MH  - *Oocytes/metabolism
MH  - RNA, Messenger/metabolism
PMC - PMC8083967
OTO - NOTNLM
OT  - meiosis
OT  - mitochondria
OT  - oocyte maturation
OT  - oxidative phosphorylation
OT  - transcriptome
COIS- No conflicts of interest, financial or otherwise, are declared by the authors.
EDAT- 2021/02/09 06:00
MHDA- 2022/03/22 06:00
PMCR- 2022/04/01
CRDT- 2021/02/08 08:42
PHST- 2021/02/09 06:00 [pubmed]
PHST- 2022/03/22 06:00 [medline]
PHST- 2021/02/08 08:42 [entrez]
PHST- 2022/04/01 00:00 [pmc-release]
AID - PG-00155-2020 [pii]
AID - 10.1152/physiolgenomics.00155.2020 [doi]
PST - ppublish
SO  - Physiol Genomics. 2021 Apr 1;53(4):137-149. doi: 
      10.1152/physiolgenomics.00155.2020. Epub 2021 Feb 8.