PMID- 33556719
OWN - NLM
STAT- MEDLINE
DCOM- 20211207
LR  - 20211214
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Linking)
VI  - 105
DP  - 2021 Feb
TI  - Involvement of signal transducers and activators of transcription in trophoblast 
      differentiation.
PG  - 94-103
LID - S0143-4004(21)00029-1 [pii]
LID - 10.1016/j.placenta.2021.01.021 [doi]
AB  - INTRODUCTION: To explore the involvement of signal transducers and activators of 
      transcription (STATs) in trophoblast differentiation. METHODS AND RESULTS: First, 
      the localization of STATs in human placentas was detected via 
      immunohistochemistry (IHC) and immunofluorescence (IF). Cytotrophoblasts (CTBs) 
      expressed both STAT1 and 3, but syncytiotrophoblasts (STBs) did not. Staining for 
      these two proteins showed a distinct upregulation from the proximal part to the 
      distal end of cell columns. STAT5B was mainly expressed in the STBs, low in the 
      CTBs, and absent in the extravillous trophoblasts (EVTs). Next, the 44 placenta 
      samples were tested via western blot (WB) and quantitative real time polymerase 
      chain reaction (qRT-PCR). We found a decrease in STAT1 and 3 and an increase in 
      STAT5B as gestation increased from five to 10 weeks. Then, an in vitro co-culture 
      model of placenta with or without decidua stromal cells (DSCs), as detected via 
      flow cytometry, revealed an increase in the human leukocyte antigen (HLA)-G 
      positive rate in trophoblasts from placentas co-cultured with DSCs, accompanied 
      by an increase in p-STAT1 and 3 and a decrease in p-STAT5 and STAT5B. Finally, 
      mRNA of matrix metalloproteinases (MMPs) and integrins after STAT silencing in 
      HTR-8/SVneo was detected via qRT-PCR. STAT1 silencing decreased MMP9 expression, 
      STAT3 silencing decreased MMP9, integrin alpha6, and beta4 expression, and STAT5B 
      silencing increased MMP2 and integrin beta1 expression. DISCUSSION: Different 
      trophoblasts showed distinct STAT expression profiles which were related to their 
      MMP and integrin expression. DSCs promoted trophoblast differentiation into EVTs, 
      possibly by regulating the STAT expression of the trophoblasts.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Chen, Chao
AU  - Chen C
AD  - Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of 
      Gynecologic Oncology, Shanghai, China.
FAU - Kang, Xiaomin
AU  - Kang X
AD  - Department of Reproductive Medical Center, The First People's Hospital of Yunnan 
      Province, Kunming, Yunnan, China.
FAU - Li, Congcong
AU  - Li C
AD  - Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of 
      Gynecologic Oncology, Shanghai, China.
FAU - Guo, Feng
AU  - Guo F
AD  - Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of 
      Gynecologic Oncology, Shanghai, China.
FAU - Wang, Qiaohong
AU  - Wang Q
AD  - Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of 
      Gynecologic Oncology, Shanghai, China.
FAU - Zhao, Aimin
AU  - Zhao A
AD  - Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of 
      Gynecologic Oncology, Shanghai, China. Electronic address: zamzkh0526@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210130
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (STAT Transcription Factors)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Adult
MH  - Cell Differentiation/*physiology
MH  - Cell Line
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinases/genetics/metabolism
MH  - Placenta/cytology/*metabolism
MH  - Pregnancy
MH  - STAT Transcription Factors/genetics/*metabolism
MH  - Stromal Cells/cytology/metabolism
MH  - Trophoblasts/cytology/*metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - Decidual stromal cell
OT  - Differentiation
OT  - Integrins
OT  - Matrix metalloproteinases
OT  - STAT transcription Factors
OT  - Trophoblast
EDAT- 2021/02/09 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/02/08 20:15
PHST- 2020/07/15 00:00 [received]
PHST- 2020/12/27 00:00 [revised]
PHST- 2021/01/27 00:00 [accepted]
PHST- 2021/02/09 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/02/08 20:15 [entrez]
AID - S0143-4004(21)00029-1 [pii]
AID - 10.1016/j.placenta.2021.01.021 [doi]
PST - ppublish
SO  - Placenta. 2021 Feb;105:94-103. doi: 10.1016/j.placenta.2021.01.021. Epub 2021 Jan 
      30.