PMID- 33557582
OWN - NLM
STAT- MEDLINE
DCOM- 20211101
LR  - 20220531
IS  - 1945-8932 (Electronic)
IS  - 1945-8932 (Linking)
VI  - 35
IP  - 6
DP  - 2021 Nov
TI  - Upregulated Expression of Toll-Like Receptor 7 in Peripheral Blood Basophils of 
      Patients With Allergic Rhinitis.
PG  - 746-760
LID - 10.1177/1945892421993034 [doi]
AB  - BACKGROUND: Recently, it has been reported that Toll-like receptor 7 (TLR7) 
      agonists can improve allergic rhinitis (AR) symptoms by up-regulation of Th1 
      cytokine release and suppression of Th2 cell functions. However, little is known 
      of the expression of TLR7 in basophils of AR. OBJECTIVE: To explore the 
      expression of TLR7 in basophils of AR, and influence of allergens on TLR7 
      expression. METHODS: The expression levels of TLR7 in basophils of patients with 
      AR were determined by flow cytometry, and the influence of allergens on TLR7 
      expression was examined by real time (q) PCR. RESULTS: The percentages of 
      TLR7(+)CCR3(+) cells (P < 0.001 and P = 0.011), TLR7(+)CD123(+)HLA-DR(-) cells 
      (P = 0 .016 and P = 0.042) and TLR7(+)CCR3(+)CD123(+)HLA-DR(-) cells (P = 0.046 
      and P = 0.035) in blood granulocyte and mononucleated cell populations of the 
      patients with AR were increased, respectively compared with HC subjects. TLR7 MFI 
      on CCR3(+) cells (P = 0.050 and P = 0.043), CD123(+)HLA-DR(-) cells (P < 0.001 
      and P = 0.002) and CCR3(+)CD123(+)HLA-DR(-) cells (P < 0.001 and P = 0.003) were 
      enhanced compared with HC subjects. Allergens Der p1 and OVA provoked 
      upregulation of TLR7 expression at both protein and mRNA levels and IL-13 
      production in KU812 cells. House Dust Mite extract (HDME), Artemisia sieversiana 
      wild allergen extract (ASWE), IL-31, IL-33, IL-37, and TSLP provoked elevation of 
      IL-6 release from KU812 cells following 2 h incubation period. CONCLUSIONS: The 
      percentage of TLR7(+) basophils and TLR7 expression intensity in a single 
      basophil are both increased in the blood of patients with AR, indicating that 
      basophils likely contribute to the pathogenesis of AR via TLR7.
FAU - Wang, Lihong
AU  - Wang L
AD  - Department of Immunology, Translational Medicine Institute, Shenyang Medical 
      College, Shenyang, China.
FAU - Zhan, Mengmeng
AU  - Zhan M
AD  - Department of Immunology, Translational Medicine Institute, Shenyang Medical 
      College, Shenyang, China.
FAU - Wang, Junling
AU  - Wang J
AD  - Allergy and Clinical Immunology Research Centre, the First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
FAU - Chen, Dong
AU  - Chen D
AD  - Allergy and Clinical Immunology Research Centre, the First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
FAU - Zhao, Nan
AU  - Zhao N
AD  - Allergy and Clinical Immunology Research Centre, the First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
FAU - Wang, Ling
AU  - Wang L
AD  - Department of Immunology, Translational Medicine Institute, Shenyang Medical 
      College, Shenyang, China.
FAU - Wang, Wei
AU  - Wang W
AD  - Allergy and Clinical Immunology Research Centre, the First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
FAU - Zhang, Xiaowen
AU  - Zhang X
AD  - Allergy and Clinical Immunology Research Centre, the First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
FAU - Huang, Yixia
AU  - Huang Y
AD  - Allergy and Clinical Immunology Research Centre, the First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
FAU - Zhang, Huiyun
AU  - Zhang H
AD  - Department of Immunology, Translational Medicine Institute, Shenyang Medical 
      College, Shenyang, China.
FAU - He, Shaoheng
AU  - He S
AUID- ORCID: 0000-0002-4230-7639
AD  - Department of Immunology, Translational Medicine Institute, Shenyang Medical 
      College, Shenyang, China.
LA  - eng
PT  - Journal Article
DEP - 20210208
PL  - United States
TA  - Am J Rhinol Allergy
JT  - American journal of rhinology & allergy
JID - 101490775
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (TLR7 protein, human)
RN  - 0 (Toll-Like Receptor 7)
SB  - IM
MH  - Allergens
MH  - *Basophils
MH  - Cytokines
MH  - Humans
MH  - *Rhinitis, Allergic
MH  - *Toll-Like Receptor 7/genetics
OTO - NOTNLM
OT  - KU812 cells
OT  - TLR7
OT  - allergen
OT  - allergic rhinitis
OT  - basophil
OT  - cytokine
EDAT- 2021/02/10 06:00
MHDA- 2021/11/03 06:00
CRDT- 2021/02/09 05:51
PHST- 2021/02/10 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
PHST- 2021/02/09 05:51 [entrez]
AID - 10.1177/1945892421993034 [doi]
PST - ppublish
SO  - Am J Rhinol Allergy. 2021 Nov;35(6):746-760. doi: 10.1177/1945892421993034. Epub 
      2021 Feb 8.