PMID- 33561150
OWN - NLM
STAT- MEDLINE
DCOM- 20210907
LR  - 20210907
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 16
IP  - 2
DP  - 2021
TI  - Transcriptome analyses reveal the utilization of nitrogen sources and related 
      metabolic mechanisms of Sporosarcina pasteurii.
PG  - e0246818
LID - 10.1371/journal.pone.0246818 [doi]
LID - e0246818
AB  - In recent years, Sporosarcina pasteurii (S. pasteurii) has become one of the most 
      popular bacteria in microbially induced calcium carbonate precipitation (MICP). 
      Various applications have been developed based on the efficient urease that can 
      induce the precipitation of calcium carbonate. However, the metabolic mechanism 
      related to biomineralization of S. pasteurii has not been clearly elucidated. The 
      process of bacterial culture and biomineralization consumes a large amount of 
      urea or ammonium salts, which are usually used as agricultural fertilizers, not 
      to mention probable environmental pollutions caused by the excessive use of these 
      raw materials. Therefore, it is urgent to reveal the mechanism of nitrogen 
      utilization and metabolism of S. pasteurii. In this paper, we compared the growth 
      and gene expression of S. pasteurii under three different culture conditions 
      through transcriptome analyses. GO and KEGG analyses revealed that both ammonium 
      and urea were direct nitrogen sources of S. pasteurii, and the bacteria could not 
      grow normally in the absence of ammonium or urea. To the best of our knowledge, 
      this paper is the first one to reveal the nitrogen utilization mechanism of S. 
      pasteurii through transcriptome methods. Furthermore, the presence of ammonium 
      might promote the synthesis of intracellular ATP and enhance the motility of the 
      bacteria. There should be an ATP synthesis mechanism associated with urea 
      hydrolysis catalyzed by urease in S. pasteurii.
FAU - Pei, Di
AU  - Pei D
AUID- ORCID: 0000-0001-6690-844X
AD  - College of Liberal Arts and Sciences, National University of Defense Technology, 
      Changsha, Hunan, China.
FAU - Liu, Zhiming
AU  - Liu Z
AD  - College of Liberal Arts and Sciences, National University of Defense Technology, 
      Changsha, Hunan, China.
AD  - Institute of Chemical Defense, Academy of Military Science, Beijing, China.
FAU - Wu, Wenjian
AU  - Wu W
AD  - College of Liberal Arts and Sciences, National University of Defense Technology, 
      Changsha, Hunan, China.
FAU - Hu, Biru
AU  - Hu B
AD  - College of Liberal Arts and Sciences, National University of Defense Technology, 
      Changsha, Hunan, China.
LA  - eng
PT  - Journal Article
DEP - 20210209
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Ammonium Compounds)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 8W8T17847W (Urea)
RN  - EC 3.5.1.5 (Urease)
RN  - N762921K75 (Nitrogen)
RN  - Sporosarcina pasteurii
SB  - IM
MH  - Adenosine Triphosphate/biosynthesis
MH  - Ammonium Compounds/pharmacology
MH  - Biosynthetic Pathways/drug effects/genetics
MH  - Cell Wall/drug effects/genetics
MH  - Down-Regulation/drug effects/genetics
MH  - Flagella/drug effects/genetics
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation, Bacterial/drug effects
MH  - Gene Ontology
MH  - Genes, Bacterial
MH  - Nitrogen/*pharmacology
MH  - Sporosarcina/drug effects/*genetics/growth & development/*metabolism
MH  - Transcriptome/drug effects/genetics
MH  - Up-Regulation/drug effects/genetics
MH  - Urea/pharmacology
MH  - Urease/genetics/metabolism
PMC - PMC7872227
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/02/10 06:00
MHDA- 2021/09/08 06:00
PMCR- 2021/02/09
CRDT- 2021/02/09 17:12
PHST- 2020/10/08 00:00 [received]
PHST- 2021/01/26 00:00 [accepted]
PHST- 2021/02/09 17:12 [entrez]
PHST- 2021/02/10 06:00 [pubmed]
PHST- 2021/09/08 06:00 [medline]
PHST- 2021/02/09 00:00 [pmc-release]
AID - PONE-D-20-31602 [pii]
AID - 10.1371/journal.pone.0246818 [doi]
PST - epublish
SO  - PLoS One. 2021 Feb 9;16(2):e0246818. doi: 10.1371/journal.pone.0246818. 
      eCollection 2021.