PMID- 33561425
OWN - NLM
STAT- MEDLINE
DCOM- 20210519
LR  - 20210519
IS  - 1875-9777 (Electronic)
IS  - 1875-9777 (Linking)
VI  - 28
IP  - 4
DP  - 2021 Apr 1
TI  - Endothelium-derived stromal cells contribute to hematopoietic bone marrow niche 
      formation.
PG  - 653-670.e11
LID - S1934-5909(21)00006-0 [pii]
LID - 10.1016/j.stem.2021.01.006 [doi]
AB  - Bone marrow stromal cells (BMSCs) play pivotal roles in tissue maintenance and 
      regeneration. Their origins, however, remain incompletely understood. Here we 
      identify rare LNGFR(+) cells in human fetal and regenerative bone marrow that 
      co-express endothelial and stromal markers. This endothelial subpopulation 
      displays transcriptional reprogramming consistent with endothelial-to-mesenchymal 
      transition (EndoMT) and can generate multipotent stromal cells that reconstitute 
      the bone marrow (BM) niche upon transplantation. Single-cell transcriptomics and 
      lineage tracing in mice confirm robust and sustained contributions of EndoMT to 
      bone precursor and hematopoietic niche pools. Interleukin-33 (IL-33) is 
      overexpressed in subsets of EndoMT cells and drives this conversion process 
      through ST2 receptor signaling. These data reveal generation of tissue-forming 
      BMSCs from mouse and human endothelial cells and may be instructive for 
      approaches to human tissue regeneration.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Kenswil, Keane Jared Guillaume
AU  - Kenswil KJG
AD  - Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015 CN, the 
      Netherlands.
FAU - Pisterzi, Paola
AU  - Pisterzi P
AD  - Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015 CN, the 
      Netherlands.
FAU - Sanchez-Duffhues, Gonzalo
AU  - Sanchez-Duffhues G
AD  - Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden 
      2300 RC, the Netherlands.
FAU - van Dijk, Claire
AU  - van Dijk C
AD  - Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015 CN, the 
      Netherlands.
FAU - Lolli, Andrea
AU  - Lolli A
AD  - Department of Oral and Maxillofacial Surgery, Erasmus MC, University Medical 
      Center Rotterdam, Rotterdam 3000 DR, the Netherlands.
FAU - Knuth, Callie
AU  - Knuth C
AD  - Department of Oral and Maxillofacial Surgery, Erasmus MC, University Medical 
      Center Rotterdam, Rotterdam 3000 DR, the Netherlands.
FAU - Vanchin, Byambasuren
AU  - Vanchin B
AD  - Cardiovascular Regenerative Medicine Research Group, Department of Pathology and 
      Medical Biology, University Medical Center Groningen, University of Groningen, 
      Groningen 9713 GZ, the Netherlands.
FAU - Jaramillo, Adrian Christopher
AU  - Jaramillo AC
AD  - Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015 CN, the 
      Netherlands.
FAU - Hoogenboezem, Remco Michiel
AU  - Hoogenboezem RM
AD  - Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015 CN, the 
      Netherlands.
FAU - Sanders, Mathijs Arnoud
AU  - Sanders MA
AD  - Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015 CN, the 
      Netherlands.
FAU - Feyen, Jacqueline
AU  - Feyen J
AD  - Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015 CN, the 
      Netherlands.
FAU - Cupedo, Tom
AU  - Cupedo T
AD  - Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015 CN, the 
      Netherlands.
FAU - Costa, Ivan G
AU  - Costa IG
AD  - Institute for Computational Genomics, Joint Research Center for Computational 
      Biomedicine, RWTH Aachen, Aachen 52074, Germany.
FAU - Li, Ronghui
AU  - Li R
AD  - Institute for Computational Genomics, Joint Research Center for Computational 
      Biomedicine, RWTH Aachen, Aachen 52074, Germany.
FAU - Bindels, Eric Monique Johannes
AU  - Bindels EMJ
AD  - Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015 CN, the 
      Netherlands.
FAU - Lodder, Kirsten
AU  - Lodder K
AD  - Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden 
      2300 RC, the Netherlands.
FAU - Blom, Bianca
AU  - Blom B
AD  - Amsterdam UMC, University of Amsterdam, Department of Experimental Immunology, 
      Amsterdam institute for Infection & Immunity, Amsterdam 1105 AZ, the Netherlands.
FAU - Bos, Pieter Koen
AU  - Bos PK
AD  - Department of Orthopaedics, Erasmus MC, Rotterdam 3015CE, the Netherlands.
FAU - Goumans, Marie-Jose
AU  - Goumans MJ
AD  - Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden 
      2300 RC, the Netherlands.
FAU - Ten Dijke, Peter
AU  - Ten Dijke P
AD  - Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden 
      2300 RC, the Netherlands; Oncode Institute, Leiden University Medical Centre, 
      Leiden 2300 RC, the Netherlands.
FAU - Farrell, Eric
AU  - Farrell E
AD  - Department of Oral and Maxillofacial Surgery, Erasmus MC, University Medical 
      Center Rotterdam, Rotterdam 3000 DR, the Netherlands.
FAU - Krenning, Guido
AU  - Krenning G
AD  - Cardiovascular Regenerative Medicine Research Group, Department of Pathology and 
      Medical Biology, University Medical Center Groningen, University of Groningen, 
      Groningen 9713 GZ, the Netherlands.
FAU - Raaijmakers, Marc Hermanus Gerardus Petrus
AU  - Raaijmakers MHGP
AD  - Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015 CN, the 
      Netherlands. Electronic address: m.h.g.raaijmakers@erasmusmc.nl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210208
PL  - United States
TA  - Cell Stem Cell
JT  - Cell stem cell
JID - 101311472
SB  - IM
MH  - Animals
MH  - *Bone Marrow
MH  - Bone Marrow Cells
MH  - Endothelial Cells
MH  - Endothelium
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells
MH  - Mice
MH  - Stromal Cells
OTO - NOTNLM
OT  - EndoMT
OT  - development
OT  - endothelial
OT  - endothelial-mesenchymal transition
OT  - hematopoietic stem cell
OT  - mesenchymal
OT  - niche
OT  - regeneration
OT  - stroma
OT  - transdifferentiation
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2021/02/10 06:00
MHDA- 2021/05/20 06:00
CRDT- 2021/02/09 20:09
PHST- 2020/03/20 00:00 [received]
PHST- 2020/09/29 00:00 [revised]
PHST- 2021/01/11 00:00 [accepted]
PHST- 2021/02/10 06:00 [pubmed]
PHST- 2021/05/20 06:00 [medline]
PHST- 2021/02/09 20:09 [entrez]
AID - S1934-5909(21)00006-0 [pii]
AID - 10.1016/j.stem.2021.01.006 [doi]
PST - ppublish
SO  - Cell Stem Cell. 2021 Apr 1;28(4):653-670.e11. doi: 10.1016/j.stem.2021.01.006. 
      Epub 2021 Feb 8.