PMID- 33563553
OWN - NLM
STAT- MEDLINE
DCOM- 20210603
LR  - 20240226
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 93
DP  - 2021 Apr
TI  - Astragaloside IV ameliorates steroid-induced osteonecrosis of the femoral head by 
      repolarizing the phenotype of pro-inflammatory macrophages.
PG  - 107345
LID - S1567-5769(20)33813-3 [pii]
LID - 10.1016/j.intimp.2020.107345 [doi]
AB  - Osteonecrosis of the femoral head (ON-FH) is a common complication of steroid 
      use. Pro-inflammatory macrophages play a crucial role in the apoptosis of 
      osteocytes. The objective of the study was to evaluate a plant extract 
      astragaloside IV (AS-IV) in treating ON-FN. Bone-marrow-derived macrophages 
      (BMDMs) were treated with lipopolysaccharides (LPS), IFN-gamma or IL-4 to induce M1 
      and M2-like phenotypes. Quantitative real-time PCR and Western blot were used to 
      examine M1 and M2 phenotypic markers. Flow cytometry was used to analyze MHC II, 
      CD206, F4/80, and CD11b levels and cell apoptosis. Glucocorticoid was used to 
      induce ON-FN in mice. TNF-alpha and IL-1beta levels in femoral head were determined 
      using enzyme-linked immunosorbent assay. AS-IV repolarized macrophages from M1 to 
      M2 phenotypes. Culture medium from AS-IV treated M1 macrophages induced less cell 
      apoptosis osteocytes compared to that from untreated M1 macrophages. In ON-FH 
      mice, the ratio of M1 macrophages was decreased in the femoral head by AS-IV, 
      concomitant with a decrease in TNF-alpha and IL-1beta levels. AS-IV is effective in 
      alleviating ON-FH through its effects in repolarizing macrophages from M1-like 
      phenotype to M2-like phenotype, promoting survival of osteocytes, reducing 
      arthritic symptoms, and decreasing inflammatory cytokines.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Jiang, Chaolai
AU  - Jiang C
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Zhou, Zubin
AU  - Zhou Z
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Lin, Yiwei
AU  - Lin Y
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Shan, Haojie
AU  - Shan H
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Xia, Wenyang
AU  - Xia W
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Yin, Fuli
AU  - Yin F
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Wang, Nan
AU  - Wang N
AD  - Department of Emergency, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan, China.
FAU - Zhou, Lihui
AU  - Zhou L
AD  - Department of Orthopaedic Surgery, Xiangshan First People's Hospital, Ningbo 
      315700, Zhejiang, China.
FAU - Gao, Youshui
AU  - Gao Y
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Yu, Xiaowei
AU  - Yu X
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China. Electronic address: yuxw@sjtu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210206
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Glucocorticoids)
RN  - 0 (Saponins)
RN  - 0 (Triterpenes)
RN  - 3A592W8XKE (astragaloside A)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Female
MH  - Femur Head/drug effects/immunology
MH  - Femur Head Necrosis/chemically induced/*drug therapy/immunology
MH  - Glucocorticoids
MH  - Macrophages/*drug effects/immunology
MH  - Mice, Inbred C57BL
MH  - Phenotype
MH  - Saponins/pharmacology/*therapeutic use
MH  - Triterpenes/pharmacology/*therapeutic use
MH  - Mice
OTO - NOTNLM
OT  - Astragaloside IV
OT  - Inflammation
OT  - Macrophages
OT  - Osteonecrosis of the femoral head
OT  - Repolarization
EDAT- 2021/02/11 06:00
MHDA- 2021/06/04 06:00
CRDT- 2021/02/10 05:39
PHST- 2020/07/24 00:00 [received]
PHST- 2020/12/21 00:00 [revised]
PHST- 2020/12/24 00:00 [accepted]
PHST- 2021/02/11 06:00 [pubmed]
PHST- 2021/06/04 06:00 [medline]
PHST- 2021/02/10 05:39 [entrez]
AID - S1567-5769(20)33813-3 [pii]
AID - 10.1016/j.intimp.2020.107345 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2021 Apr;93:107345. doi: 10.1016/j.intimp.2020.107345. Epub 
      2021 Feb 6.