PMID- 33566037
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 146
IP  - 6
DP  - 2021 Mar 21
TI  - Noble-metal nanoparticle labelling multiplex miRNAs by ICP-MS readout with 
      internal standard isotopes of (115)In and (209)Bi.
PG  - 2074-2082
LID - 10.1039/d0an01975e [doi]
AB  - Inductively coupled plasma-mass spectrometry (ICP-MS) is one of the most powerful 
      techniques for multiplex nucleotide assay owing to the virtue of the high 
      resolution of multiple-elements' mass to charge ratio, in a mass spectrum. Here, 
      a small sized (less than 20 nm) noble-metal nanoparticle labelled ICP-MS 
      (NP-ICP-MS) is proposed for high-throughput microRNA (miRNA) determination. Three 
      miRNA targets - miR-486-5p, miR-221, and miR-21 - in serum, were distinguished by 
      single-stranded DNA (ssDNA) probes labelled with a small sized noble-metal 
      nanoparticle - silver nanoparticles (AgNPs), platinum nanoparticles (PtNPs), and 
      gold nanoparticles (AuNPs). The counting isotopes ion intensity per second (CPS) 
      of the noble-metal label versus internal standard isotope intensity of (115)In 
      and (209)Bi, exhibited good linearity in the range 0.25 pM to 100 pM with 
      correlation coefficients (R(2)) of 0.9680, 0.9305, and 0.9418. The specific 
      sandwich-type miRNA assay using the sensitive NP-ICP-MS readout pushed the 
      detection limits down to 0.18 pM for miR-221, 0.23 pM for miR-486-5p, and 0.22 pM 
      for miR-21. And the relative standard deviations (RSDs) for 10 pM target miRNA 
      were less than 3.7%. This work promises a potential ultrasensitive ICP-MS 
      bioassay of multiplex miRNA biomarkers for clinical serum diagnosis.
FAU - Zhang, Fei
AU  - Zhang F
AD  - Institute of Physicochemical Detection, Sichuan Centre for Disease Control and 
      Prevention, Chengdu, Sichuan 610041, China.
FAU - Yong, Li
AU  - Yong L
FAU - Hua, Xia
AU  - Hua X
FAU - You, Fan
AU  - You F
FAU - Wang, Bin
AU  - Wang B
FAU - Feng, Yu-Liang
AU  - Feng YL
FAU - Mao, Li
AU  - Mao L
LA  - eng
PT  - Journal Article
DEP - 20210210
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Isotopes)
RN  - 0 (MicroRNAs)
RN  - 3M4G523W1G (Silver)
RN  - 49DFR088MY (Platinum)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Gold
MH  - Isotopes
MH  - *Metal Nanoparticles
MH  - *MicroRNAs/genetics
MH  - Platinum
MH  - Silver
EDAT- 2021/02/11 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/02/10 12:10
PHST- 2021/02/11 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2021/02/10 12:10 [entrez]
AID - 10.1039/d0an01975e [doi]
PST - ppublish
SO  - Analyst. 2021 Mar 21;146(6):2074-2082. doi: 10.1039/d0an01975e. Epub 2021 Feb 10.