PMID- 33566191
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221209
IS  - 1931-7573 (Print)
IS  - 1556-276X (Electronic)
IS  - 1556-276X (Linking)
VI  - 16
IP  - 1
DP  - 2021 Feb 10
TI  - Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomal MicroRNA-18b-3p 
      Inhibits the Occurrence of Preeclampsia by Targeting LEP.
PG  - 27
LID - 10.1186/s11671-021-03475-5 [doi]
LID - 27
AB  - Exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs) 
      expressing microRNAs have been highlighted in human diseases. However, the 
      detailed molecular mechanism of hucMSCs-derived exosomal miR-18b-3p on 
      preeclampsia (PE) remains further investigation. We aimed to investigate the 
      effect of exosomes and miR-18b-3p/leptin (LEP) on occurrence of PE. The 
      morphology of the hucMSC and hucMSC-exosomes (Exos) was identified. The exosomes 
      were infected with different lentivirus expressing miR-18b-3p to explore the role 
      of miR-18b-3p in PE. The PE rat model was established by intraperitoneal 
      injection of N-nitro-L-arginine methyl ester. The expression of LEP and 
      miR-18b-3p was tested in PE rat placenta tissues. Also, the effect of exosomes on 
      LEP and miR-18b-3p expression was detected. The systolic blood pressure (SBP), 
      proteinuria, inflammatory factors, the weight of fetal rat and placenta and cell 
      apoptosis in PE rats were detected. Finally, the relationship between miR-18b-3p 
      and LEP was verified using dual-luciferase reporter gene assay and RNA pull-down 
      assay. Exosomes, restoring miR-18b-3p or inhibiting LEP reduced SBP and 
      proteinuria of PE rats as well as increased the weight of fetal rat and placenta, 
      decreased serum levels of inflammatory factors as well as suppressed apoptotic 
      cells of PE rats, exerting a suppressive effect on PE progression. miR-18b-3p was 
      decreased and LEP was increased in placenta tissues of PE rats. LEP was the 
      direct target gene of miR-18b-3p. Upregulation of miR-18b-3p or treatment of the 
      exosomes suppressed LEP expression and reduced PE occurrence, while 
      downregulation of miR-18b-3p had contrary effects. Downregulated LEP reversed the 
      effect of miR-18b-3p reduction on PE rats. HucMSCs-derived exosomal miR-18b-3p 
      targets LEP to participate in the occurrence and development of PE. This study 
      may provide a novel theoretical basis for the mechanism and investigation of PE.
FAU - Huang, Qin
AU  - Huang Q
AD  - Department of Obstetrics and Gynecology, Renmin Hosptial of Wuhan University, 238 
      Jiefang Road, Wuchang District, Wuhan, 430060, Hubei, China.
FAU - Gong, Meng
AU  - Gong M
AD  - Department of Obstetrics and Gynecology, Renmin Hosptial of Wuhan University, 238 
      Jiefang Road, Wuchang District, Wuhan, 430060, Hubei, China.
FAU - Tan, Tuantuan
AU  - Tan T
AD  - Ultrasound Imaging Department, Renmin Hosptial of Wuhan University, Wuhan, 
      430060, Hubei, China.
FAU - Lin, Yunong
AU  - Lin Y
AD  - Department of Statistics, UW-Madison, Madison, 53703, USA.
FAU - Bao, Yan
AU  - Bao Y
AD  - Department of Obstetrics and Gynecology, Renmin Hosptial of Wuhan University, 238 
      Jiefang Road, Wuchang District, Wuhan, 430060, Hubei, China.
FAU - Fan, Cuifang
AU  - Fan C
AD  - Department of Obstetrics and Gynecology, Renmin Hosptial of Wuhan University, 238 
      Jiefang Road, Wuchang District, Wuhan, 430060, Hubei, China. 
      fancuifang@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20210210
PL  - United States
TA  - Nanoscale Res Lett
JT  - Nanoscale research letters
JID - 101279750
RIN - Nanoscale Res Lett. 2022 Dec 9;17(1):119. PMID: 36484896
PMC - PMC7876216
OTO - NOTNLM
OT  - Apoptosis
OT  - Exosomes
OT  - Human umbilical cord mesenchymal stem cells
OT  - Leptin
OT  - MicroRNA-18b-3p
OT  - Preeclampsia
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2021/02/11 06:00
MHDA- 2021/02/11 06:01
PMCR- 2021/02/10
CRDT- 2021/02/10 12:11
PHST- 2020/07/03 00:00 [received]
PHST- 2021/01/05 00:00 [accepted]
PHST- 2021/02/10 12:11 [entrez]
PHST- 2021/02/11 06:00 [pubmed]
PHST- 2021/02/11 06:01 [medline]
PHST- 2021/02/10 00:00 [pmc-release]
AID - 10.1186/s11671-021-03475-5 [pii]
AID - 3475 [pii]
AID - 10.1186/s11671-021-03475-5 [doi]
PST - epublish
SO  - Nanoscale Res Lett. 2021 Feb 10;16(1):27. doi: 10.1186/s11671-021-03475-5.