PMID- 33566442
OWN - NLM
STAT- MEDLINE
DCOM- 20211119
LR  - 20211119
IS  - 1759-7714 (Electronic)
IS  - 1759-7706 (Print)
IS  - 1759-7706 (Linking)
VI  - 12
IP  - 7
DP  - 2021 Apr
TI  - Chaperone-mediated autophagy affects tumor cell proliferation and cisplatin 
      resistance in esophageal squamous cell carcinoma.
PG  - 1048-1057
LID - 10.1111/1759-7714.13849 [doi]
AB  - BACKGROUND: Chaperone-mediated autophagy (CMA) is a lysosomal degradation pathway 
      of selective soluble proteins. Lysosomal membrane associated protein 2a (LAMP2a) 
      is the lysosomal membrane receptor of CMA and influences CMA activity. Although 
      it has been suggested that higher expression of LAMP2a is associated with more 
      advanced tumor node metastasis (TNM) stages and shorter survival time in patients 
      with esophageal squamous cell carcinoma (ESCC), the underlying mechanism has not 
      been known yet. METHODS: In this study, we modulated the activity of CMA through 
      LAMP2a or small molecular compounds in human ESCC cells to investigate its role 
      in ESCC. RESULTS: We found that down-regulating the activity of CMA could inhibit 
      the proliferation and colony formation of ESCC cells as well as increase their 
      sensitivity to cisplatin. CONCLUSIONS: Our results promote better understanding 
      of how CMA affects human ESCC and provide a new therapeutic target against ESCC 
      through down-regulating LAMP2a.
CI  - (c) 2021 The Authors. Thoracic Cancer published by China Lung Oncology Group and 
      John Wiley & Sons Australia, Ltd.
FAU - Cao, Dujuan
AU  - Cao D
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Shan, Danyang
AU  - Shan D
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Yan, Wenli
AU  - Yan W
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Zhang, Zifang
AU  - Zhang Z
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Song, Qianqian
AU  - Song Q
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Jiang, Yuanyuan
AU  - Jiang Y
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Zhang, Xinmiao
AU  - Zhang X
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Zhang, Zhongxian
AU  - Zhang Z
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Wang, Zhimin
AU  - Wang Z
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Wang, Yaohe
AU  - Wang Y
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
AD  - Centre for Biomarkers & Biotherapeutics, Barts Cancer Institute, Queen Mary 
      University of London, London, UK.
FAU - Lu, Shuangshuang
AU  - Lu S
AUID- ORCID: 0000-0002-8144-1830
AD  - National Center for International Research in Cell and Gene Therapy, Sino-British 
      Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy 
      of Medical Sciences, Zhengzhou University, Zhengzhou, China.
LA  - eng
GR  - This project is supported by the National Key R&D program of China 
      (2016YFE0200800), the Department of Education of Henan Province, key scientific 
      research projects of Henan Province colleges and universities (21A310023), the 
      Nature Sciences Foundation of China (U1704282, 81771776), and the core funding 
      for development of the Cell and Gene Therapy Program by Zhengzhou University 
      Dujuan Cao and Danyang Shan contributed equally to this work/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210210
PL  - Singapore
TA  - Thorac Cancer
JT  - Thoracic cancer
JID - 101531441
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Chaperone-Mediated Autophagy/*genetics
MH  - Drug Resistance, Neoplasm/*genetics
MH  - Esophageal Squamous Cell Carcinoma/*drug therapy
MH  - Female
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Signal Transduction
MH  - Transfection
MH  - Xenograft Model Antitumor Assays
PMC - PMC8017246
OTO - NOTNLM
OT  - cell proliferation
OT  - chaperone-mediated autophagy
OT  - cisplatin resistance
OT  - esophageal squamous cell carcinoma
OT  - lysosomal membrane associated protein 2a
COIS- All authors declare no potential conflict of interests.
EDAT- 2021/02/11 06:00
MHDA- 2021/11/20 06:00
PMCR- 2021/04/01
CRDT- 2021/02/10 12:14
PHST- 2021/01/03 00:00 [revised]
PHST- 2020/12/18 00:00 [received]
PHST- 2021/01/04 00:00 [accepted]
PHST- 2021/02/11 06:00 [pubmed]
PHST- 2021/11/20 06:00 [medline]
PHST- 2021/02/10 12:14 [entrez]
PHST- 2021/04/01 00:00 [pmc-release]
AID - TCA13849 [pii]
AID - 10.1111/1759-7714.13849 [doi]
PST - ppublish
SO  - Thorac Cancer. 2021 Apr;12(7):1048-1057. doi: 10.1111/1759-7714.13849. Epub 2021 
      Feb 10.