PMID- 33566740
OWN - NLM
STAT- MEDLINE
DCOM- 20220310
LR  - 20220311
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 34
IP  - 4
DP  - 2021 May
TI  - The Role of Autophagy in Murine Cytomegalovirus Hepatitis.
PG  - 241-255
LID - 10.1089/vim.2020.0024 [doi]
AB  - Autophagy is involved in the pathogenesis of multiple pathogen infection. 
      Previous studies have reported that human cytomegalovirus (CMV) activates 
      autophagy in the early stage of infection and then inhibits autophagy. Little is 
      known about the role of autophagy in murine CMV (MCMV) infection, especially in 
      MCMV-induced hepatitis. The purpose of this study is to investigate the role of 
      autophagy in MCMV hepatitis. BALB/c mice were infected with MCMV and a series of 
      experiments involving western blot, immunofluorescence, immunohistochemistry, H&E 
      (Hematoxylin and Eosin) staining and quantitative real-time polymerase chain 
      reaction were performed in this study. The expression of SQSTM1/p62, PI3K, the 
      ratio of phosphorylated Akt to total Akt, and the ratio of phosphorylated 
      mammalian target of rapamycin (mTOR) to total mTOR were increased, and the 
      expression of light-chain 3 (LC3)-II were decreased in the livers of infected 
      mice on days 3 and 7 postinfection (p.i.). Compared with the untreated infected 
      group, increased transcription level of MCMV glycoprotein B (gB), increased 
      expression levels of interleukin1-beta (IL-1beta), aspartate aminotransferase (AST) and 
      alanine aminotransferase (ALT), decreased expression level of type I interferon alpha 
      (IFN-alpha), as well as aggravated liver pathological injury were detected in 
      starvation-treated infected group on days 3 and 7 p.i.; whereas decreased 
      transcription level of MCMV gB, decreased expression levels of IL-1beta, AST and 
      ALT, increased expression level of type I IFN-alpha, as well as alleviated liver 
      pathological injury were detected in chloroquine (CQ)-treated infected group on 
      day 3 p.i. In conclusion, autophagy is inhibited through activating the 
      PI3K/Akt/mTOR pathway in the liver of BALB/c mice during MCMV infection, and 
      autophagy may promote MCMV replication and aggravate liver pathological damage 
      and inflammation. Further understanding of the interactions between autophagy and 
      MCMV infection and its potential mechanism may bring new important cues to the 
      control of MCMV infection and antiviral therapy.
FAU - Zhang, Lin-Lin
AU  - Zhang LL
AD  - Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Zhang, Xin-Yan
AU  - Zhang XY
AD  - Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Lu, Yuan-Yuan
AU  - Lu YY
AD  - Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Bi, Yi-Dan
AU  - Bi YD
AD  - Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Liu, Xing-Lou
AU  - Liu XL
AD  - Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Fang, Feng
AU  - Fang F
AD  - Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210209
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
SB  - IM
MH  - Animals
MH  - Autophagy
MH  - Cytomegalovirus/physiology
MH  - *Cytomegalovirus Infections/complications
MH  - *Hepatitis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Muromegalovirus
MH  - Phosphatidylinositol 3-Kinases/metabolism
OTO - NOTNLM
OT  - LC3-II
OT  - autophagy
OT  - hepatitis
OT  - murine cytomegalovirus
OT  - p62
EDAT- 2021/02/11 06:00
MHDA- 2022/03/11 06:00
CRDT- 2021/02/10 17:11
PHST- 2021/02/11 06:00 [pubmed]
PHST- 2022/03/11 06:00 [medline]
PHST- 2021/02/10 17:11 [entrez]
AID - 10.1089/vim.2020.0024 [doi]
PST - ppublish
SO  - Viral Immunol. 2021 May;34(4):241-255. doi: 10.1089/vim.2020.0024. Epub 2021 Feb 
      9.