PMID- 33570661
OWN - NLM
STAT- MEDLINE
DCOM- 20210909
LR  - 20210909
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 479
IP  - 2
DP  - 2021 Aug
TI  - GATA3 is a useful immunohistochemical marker for distinguishing sarcomatoid 
      malignant mesothelioma from lung sarcomatoid carcinoma and organizing pleuritis.
PG  - 257-263
LID - 10.1007/s00428-021-03048-y [doi]
AB  - Sarcomatoid malignant mesothelioma (SMM) tends to occur in the pleura and is 
      morphologically similar to lung sarcomatoid carcinoma (LSC) and organizing 
      pleuritis (OP). Because SMM often does not express mesothelial markers, it is 
      very difficult to distinguish from LSC and OP. GATA-binding protein 3 (GATA3) is 
      a specific immunohistochemical (IHC) marker of breast and urothelial carcinoma. 
      We routinely find that GATA is expressed in MM; however, GATA3 expression in SMM 
      and its reference value for distinguishing SMM from LSC and OP remain unclear. 
      Here, we used IHC methods to detect the expression of GATA3 and classic 
      mesothelial markers in 17 SMM, 12 LSC, and 7 OP cases. We detected the following 
      expression rates in SMM versus LSC cases: GATA3 (70.6% vs. 16.7%, p = 0.008), 
      calretinin (52.9% vs. 8.3%, p = 0.019), Wilms tumor (WT)-1 (64.7% vs. 0%, p = 
      0.000), D2-40 (47.1% vs. 16.7%, p = 0.126), CK5/6 (35.3% vs. 25.0%, p = 0.694), 
      and pan-cytokeratin (CKpan) (88.2% vs. 100.0%, p = 0.498). The specificities of 
      calretinin, WT-1, and GATA3 in distinguishing SMM from LSC were 91.7%, 100%, and 
      83.3%, respectively, and combinations of any two of these three markers exhibited 
      100% specificity for SMM. Notably, the sensitivity of calretinin+/WT1+ staining 
      for SMM was only 23.5%, which increased to 64.7% after including GATA3. 
      Furthermore, all OP cases showed partial or diffuse expression of CKpan, WT-1, 
      and D2-40 but no GATA3 and calretinin expression. In conclusion, GATA3 is an IHC 
      marker with excellent sensitivity and specificity for SMM, and the combined 
      consideration of GATA3, calretinin, and WT-1 was best for distinguishing SMM from 
      LSC. Moreover, CKpan, WT-1, and D2-40 had no value for distinguishing SMM from 
      OP, and GATA3 and calretinin were the most specific markers for distinguishing 
      these two lesions.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part 
      of Springer Nature.
FAU - Piao, Zheng Hua
AU  - Piao ZH
AD  - Department of Pathology, Ningbo Diagnostic Pathology Center, Huan Cheng Bei Lu, 
      Jiang Bei District, Ningbo, 315000, Zhejiang, China. zhpiao0318@163.com.
FAU - Zhou, Xin Cheng
AU  - Zhou XC
AD  - Department of Pathology, Ningbo Diagnostic Pathology Center, Huan Cheng Bei Lu, 
      Jiang Bei District, Ningbo, 315000, Zhejiang, China.
FAU - Chen, Jia Yi
AU  - Chen JY
AD  - Department of Pathology, Ningbo Diagnostic Pathology Center, Huan Cheng Bei Lu, 
      Jiang Bei District, Ningbo, 315000, Zhejiang, China.
LA  - eng
PT  - Journal Article
DEP - 20210211
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CALB2 protein, human)
RN  - 0 (Calbindin 2)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (GATA3 protein, human)
RN  - 0 (WT1 Proteins)
RN  - 0 (WT1 protein, human)
SB  - IM
EIN - Virchows Arch. 2021 Mar 12;:. PMID: 33712881
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*analysis
MH  - Calbindin 2/analysis
MH  - Carcinoma/*chemistry/pathology
MH  - Diagnosis, Differential
MH  - Female
MH  - GATA3 Transcription Factor/*analysis
MH  - Humans
MH  - *Immunohistochemistry
MH  - Lung Neoplasms/*chemistry/pathology
MH  - Male
MH  - Mesothelioma, Malignant/*chemistry/pathology
MH  - Middle Aged
MH  - Pleurisy/*metabolism/pathology
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
MH  - Sarcoma/*chemistry/pathology
MH  - WT1 Proteins/analysis
MH  - Young Adult
OTO - NOTNLM
OT  - GATA3
OT  - Immunohistochemistry
OT  - Lung sarcomatoid carcinoma
OT  - Organizing pleuritis
OT  - Pathologic diagnosis
OT  - Sarcomatoid malignant mesothelioma
EDAT- 2021/02/12 06:00
MHDA- 2021/09/10 06:00
CRDT- 2021/02/11 12:11
PHST- 2020/11/10 00:00 [received]
PHST- 2021/01/28 00:00 [accepted]
PHST- 2021/01/09 00:00 [revised]
PHST- 2021/02/12 06:00 [pubmed]
PHST- 2021/09/10 06:00 [medline]
PHST- 2021/02/11 12:11 [entrez]
AID - 10.1007/s00428-021-03048-y [pii]
AID - 10.1007/s00428-021-03048-y [doi]
PST - ppublish
SO  - Virchows Arch. 2021 Aug;479(2):257-263. doi: 10.1007/s00428-021-03048-y. Epub 
      2021 Feb 11.