PMID- 33572209
OWN - NLM
STAT- MEDLINE
DCOM- 20210302
LR  - 20210316
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 13
IP  - 2
DP  - 2021 Feb 9
TI  - Porcine Circovirus 3 Detection in Aborted Fetuses and Stillborn Piglets from 
      Swine Reproductive Failure Cases.
LID - 10.3390/v13020264 [doi]
LID - 264
AB  - Porcine circovirus 3 (PCV-3) has been widely detected in healthy and diseased 
      pigs; among different pathologic conditions, the strongest evidence of 
      association comes from reproductive disease cases. However, simple viral 
      detection does not imply the causality of the clinical conditions. Detection of 
      PCV-3 within lesions may provide stronger evidence of causality. Thus, this study 
      aimed to assess the frequency of PCV-3 detection in tissues from 
      fetuses/stillborn piglets in cases of reproductive problems in domestic swine, as 
      well as the histopathologic assessment of fetal tissues. Fetuses or stillborn 
      piglets from 53 cases of reproductive failure were collected and analyzed by 
      PCV-3 qPCR. The presence of porcine reproductive and respiratory syndrome virus 
      (PRRSV), porcine circovirus 2 (PCV-2), and porcine parvovirus 1 (PPV1) was also 
      checked. PCV-3 qPCR positive samples with a high viral load were tested by PCV-3 
      in situ hybridization (ISH), sequenced, and phylogenetically analyzed. PCV-3 DNA 
      was detected in 18/53 (33.9%) reproductive failure cases and in 16 of them PCV-3 
      was the only pathogen found. PCV-2 DNA was found in 5/53 (9.4%), PRRSV RNA in 
      4/53 (7.5%) and PPV1 was not detected. Four out of the six PCV-3 qPCR-positive 
      cases with Ct value <30 were positive when tested by ISH. In these samples, PCV-3 
      was detected within mild histopathologic lesions, such as arteritis and 
      periarteritis in multiple tissues. The present work emphasizes the need to 
      include PCV-3 as a potential causative agent of reproductive failure in swine.
FAU - Saporiti, Viviane
AU  - Saporiti V
AD  - IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la 
      Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain.
AD  - OIE Collaborating Centre for the Research and Control of Emerging and Re-emerging 
      Swine Diseases in Europe (IRTA-CReSA), Bellaterra, 08193 Barcelona, Spain.
FAU - Valls, Laura
AU  - Valls L
AD  - Laboratorios Hipra, S.A., Amer, 17170 Girona, Spain.
FAU - Maldonado, Jaime
AU  - Maldonado J
AD  - Laboratorios Hipra, S.A., Amer, 17170 Girona, Spain.
FAU - Perez, Monica
AU  - Perez M
AD  - IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la 
      Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain.
AD  - OIE Collaborating Centre for the Research and Control of Emerging and Re-emerging 
      Swine Diseases in Europe (IRTA-CReSA), Bellaterra, 08193 Barcelona, Spain.
FAU - Correa-Fiz, Florencia
AU  - Correa-Fiz F
AUID- ORCID: 0000-0002-9459-5871
AD  - IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la 
      Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain.
AD  - OIE Collaborating Centre for the Research and Control of Emerging and Re-emerging 
      Swine Diseases in Europe (IRTA-CReSA), Bellaterra, 08193 Barcelona, Spain.
FAU - Segales, Joaquim
AU  - Segales J
AUID- ORCID: 0000-0002-1539-7261
AD  - OIE Collaborating Centre for the Research and Control of Emerging and Re-emerging 
      Swine Diseases in Europe (IRTA-CReSA), Bellaterra, 08193 Barcelona, Spain.
AD  - Departament de Sanitat i Anatomia Animals, Universitat Autonoma de Barcelona 
      (UAB), Bellaterra, 08193 Barcelona, Spain.
AD  - UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la 
      Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain.
FAU - Sibila, Marina
AU  - Sibila M
AUID- ORCID: 0000-0003-3867-1988
AD  - IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la 
      Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain.
AD  - OIE Collaborating Centre for the Research and Control of Emerging and Re-emerging 
      Swine Diseases in Europe (IRTA-CReSA), Bellaterra, 08193 Barcelona, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210209
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Aborted Fetus/pathology/virology
MH  - Abortion, Veterinary/pathology/*virology
MH  - Animals
MH  - Animals, Domestic
MH  - Circoviridae Infections/pathology/*veterinary/virology
MH  - Circovirus/classification/genetics/*isolation & purification
MH  - DNA, Viral/genetics
MH  - Female
MH  - Genome, Viral/genetics
MH  - Phylogeny
MH  - Pregnancy
MH  - Stillbirth/veterinary
MH  - Swine
MH  - Swine Diseases/pathology/*virology
MH  - Viral Load
MH  - Viruses/classification/genetics/isolation & purification
PMC - PMC7915229
OTO - NOTNLM
OT  - aborted fetuses
OT  - histopathology
OT  - in situ hybridization
OT  - porcine circovirus 3 (PCV-3)
OT  - quantitative PCR
OT  - reproductive failure
OT  - stillborn
COIS- The authors declare no conflict of interest.
EDAT- 2021/02/13 06:00
MHDA- 2021/03/03 06:00
PMCR- 2021/02/09
CRDT- 2021/02/12 01:01
PHST- 2020/12/31 00:00 [received]
PHST- 2021/01/27 00:00 [revised]
PHST- 2021/02/05 00:00 [accepted]
PHST- 2021/02/12 01:01 [entrez]
PHST- 2021/02/13 06:00 [pubmed]
PHST- 2021/03/03 06:00 [medline]
PHST- 2021/02/09 00:00 [pmc-release]
AID - v13020264 [pii]
AID - viruses-13-00264 [pii]
AID - 10.3390/v13020264 [doi]
PST - epublish
SO  - Viruses. 2021 Feb 9;13(2):264. doi: 10.3390/v13020264.