PMID- 33573714
OWN - NLM
STAT- MEDLINE
DCOM- 20211104
LR  - 20211104
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 33
IP  - 4
DP  - 2021 Feb
TI  - Capacity of Trolox to improve the development and quality of metabolically 
      compromised bovine oocytes and embryos invitro during different windows of 
      development.
PG  - 291-304
LID - 10.1071/RD20194 [doi]
AB  - Trials to improve oocyte developmental competence under metabolic stress by using 
      antioxidants may start before or after oocyte maturation. In the present 
      conceptual study, we aimed to identify the most efficient timing of antioxidant 
      application in relation to a metabolic insult using a bovine invitro embryo 
      production model. Pathophysiological concentrations of palmitic acid (PA) were 
      used to induce metabolic stress during oocyte maturation or embryo development. 
      Trolox (TR; antioxidant) treatment prior to, during or after the PA insult was 
      tested to evaluate the protective, neutralising and rescuing capacity of TR 
      respectively. Changes in embryo developmental competence, mitochondrial activity, 
      reactive oxygen species (ROS) concentrations, blastocyst cell allocation and 
      apoptosis and cell stress-related gene expression were monitored. The improvement 
      in developmental capacity was most obvious when oocytes were preloaded with TR 
      before the PA insult. This protective effect could be explained by the observed 
      combination of increased mitochondrial activity with reduced ROS production. This 
      resulted in blastocysts with normal cell counts and apoptosis, as well as 
      increased nuclear factor erythroid 2-related factor 2 (NRF2) expression (a marker 
      for redox regulatory processes) and normalised the expression of the 
      mitochondrial transcription factor A (TFAM), a marker of mitochondrial 
      biogenesis. These results indicate that 'pretreatment' of oocytes with 
      antioxidants produces embryos that seem to be more resilient to a metabolic 
      stress insult.
FAU - De Bie, J
AU  - De Bie J
AD  - Centre for the Evaluation of Vaccination, Vaccine and Infectious Disease 
      Institute, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium.
FAU - Smits, A
AU  - Smits A
AD  - Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, 
      Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 
      B-2610 Wilrijk, Belgium.
FAU - Marei, W F A
AU  - Marei WFA
AD  - Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, 
      Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 
      B-2610 Wilrijk, Belgium; and Department of Theriogenology, Faculty of Veterinary 
      Medicine, Cairo University, Giza 12211, Egypt.
FAU - Leroy, J L M R
AU  - Leroy JLMR
AD  - Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, 
      Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 
      B-2610 Wilrijk, Belgium; and Corresponding author. Email: jo.leroy@uantwerpen.be.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Antioxidants)
RN  - 0 (Chromans)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Transcription Factors)
RN  - 0 (mitochondrial transcription factor A)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - S18UL9710X (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Apoptosis/drug effects
MH  - Blastocyst/*drug effects/metabolism/pathology
MH  - Cattle
MH  - Cells, Cultured
MH  - Chromans/*pharmacology
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Embryo Culture Techniques
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - In Vitro Oocyte Maturation Techniques
MH  - Mitochondria/drug effects/metabolism/pathology
MH  - Mitochondrial Proteins/genetics/metabolism
MH  - NF-E2-Related Factor 2/genetics/metabolism
MH  - Oocytes/*drug effects/metabolism/pathology
MH  - Oxidative Stress/*drug effects
MH  - Palmitic Acid/toxicity
MH  - Reactive Oxygen Species/*metabolism
MH  - Transcription Factors/genetics/metabolism
EDAT- 2021/02/13 06:00
MHDA- 2021/11/05 06:00
CRDT- 2021/02/12 05:40
PHST- 2020/08/21 00:00 [received]
PHST- 2020/12/01 00:00 [accepted]
PHST- 2021/02/13 06:00 [pubmed]
PHST- 2021/11/05 06:00 [medline]
PHST- 2021/02/12 05:40 [entrez]
AID - RD20194 [pii]
AID - 10.1071/RD20194 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2021 Feb;33(4):291-304. doi: 10.1071/RD20194.