PMID- 33574566
OWN - NLM
STAT- MEDLINE
DCOM- 20220125
LR  - 20221030
IS  - 1476-5497 (Electronic)
IS  - 0307-0565 (Print)
IS  - 0307-0565 (Linking)
VI  - 45
IP  - 6
DP  - 2021 Jun
TI  - Integrative analysis of physiological responses to high fat feeding with 
      diffusion tensor images and neurochemical profiles of the mouse brain.
PG  - 1203-1214
LID - 10.1038/s41366-021-00775-9 [doi]
AB  - BACKGROUND: Obesity proceeds with important physiological and microstructural 
      alterations in the brain, but the precise relationships between the diet and 
      feeding status, its physiological responses, and the observed neuroimaging 
      repercussions, remain elusive. Here, we implemented a mouse model of high fat 
      diet (HFD) feeding to explore specific associations between diet, feeding status, 
      phenotypic and endocrine repercussions, and the resulting microstructural and 
      metabolic alterations in the brain, as detected by diffusion tensor imaging (DTI) 
      and neurochemical metabolic profiling. METHODS: Brain DTI images were acquired 
      from adult male C57BL6/J mice after 6 weeks of HFD, or standard diet (SD) 
      administrations, both under the fed, and overnight fasted conditions. Metabolomic 
      profiles of the cortex (Ctx), hippocampus (Hipc), and hypothalamus (Hyp) were 
      determined by (1)H high-resolution magic angle spinning (HRMAS) spectroscopy, in 
      cerebral biopsies dissected after microwave fixation. Mean diffusivity (MD), 
      fractional anisotropy (FA) maps, and HRMAS profiles were complemented with 
      determinations of phenotypic alterations and plasma levels of appetite-related 
      hormones, measured by indirect calorimetry and multiplex assays, respectively. We 
      used Z-score and alternating least squares scaling (ALSCAL) analysis to 
      investigate specific associations between diet and feeding status, physiological, 
      and imaging parameters. RESULTS: HFD induced significant increases in body weight 
      and the plasma levels of glucose and fatty acids in the fed and fasted 
      conditions, as well as higher cerebral MD (Ctx, Hipc, Hyp), FA (Hipc), and mobile 
      saturated fatty acids resonances (Ctx, Hipc, Hyp). Z-score and ASLCAL analysis 
      identified the precise associations between physiological and imaging variables. 
      CONCLUSIONS: The present study reveals that diet and feeding conditions elicit 
      prominent effects on specific imaging and spectroscopic parameters of the mouse 
      brain that can be associated to the alterations in phenotypic and endocrine 
      variables. Together, present results disclose a neuro-inflammatory response to 
      HFD, characterized primarily by vasogenic edema and compensatory responses in 
      osmolyte concentrations.
FAU - Guadilla, Irene
AU  - Guadilla I
AUID- ORCID: 0000-0002-3932-0840
AD  - Instituto de Investigaciones Biomedicas "Alberto Sols" CSIC-UAM, Madrid, Spain.
FAU - Lizarbe, Blanca
AU  - Lizarbe B
AD  - Instituto de Investigaciones Biomedicas "Alberto Sols" CSIC-UAM, Madrid, Spain.
FAU - Barrios, Laura
AU  - Barrios L
AD  - Centro Tecnico de Informatica CSIC, Madrid, Spain.
FAU - Cerdan, Sebastian
AU  - Cerdan S
AUID- ORCID: 0000-0001-9965-0270
AD  - Instituto de Investigaciones Biomedicas "Alberto Sols" CSIC-UAM, Madrid, Spain.
FAU - Lopez-Larrubia, Pilar
AU  - Lopez-Larrubia P
AUID- ORCID: 0000-0002-5141-8736
AD  - Instituto de Investigaciones Biomedicas "Alberto Sols" CSIC-UAM, Madrid, Spain. 
      plopez@iib.uam.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210211
PL  - England
TA  - Int J Obes (Lond)
JT  - International journal of obesity (2005)
JID - 101256108
SB  - IM
MH  - Animals
MH  - Body Weight/physiology
MH  - *Brain/diagnostic imaging/physiology
MH  - Brain Chemistry/*physiology
MH  - *Diet, High-Fat
MH  - *Diffusion Tensor Imaging
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
PMC - PMC8159736
COIS- The authors declare that they have no conflict of interest.
EDAT- 2021/02/13 06:00
MHDA- 2022/01/27 06:00
PMCR- 2021/02/11
CRDT- 2021/02/12 06:02
PHST- 2020/10/08 00:00 [received]
PHST- 2021/01/21 00:00 [accepted]
PHST- 2020/12/28 00:00 [revised]
PHST- 2021/02/13 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2021/02/12 06:02 [entrez]
PHST- 2021/02/11 00:00 [pmc-release]
AID - 10.1038/s41366-021-00775-9 [pii]
AID - 775 [pii]
AID - 10.1038/s41366-021-00775-9 [doi]
PST - ppublish
SO  - Int J Obes (Lond). 2021 Jun;45(6):1203-1214. doi: 10.1038/s41366-021-00775-9. 
      Epub 2021 Feb 11.