PMID- 33576368
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Linking)
VI  - 9
IP  - 7
DP  - 2021 Apr 7
TI  - A "sandwich" cell culture platform with NIR-responsive dynamic stiffness to 
      modulate macrophage phenotypes.
PG  - 2553-2561
LID - 10.1039/d0bm02194f [doi]
AB  - Considering the key roles of macrophages in tissue repair and immune therapy, 
      designing smart biomaterials able to harness macrophage phenotypes on demand 
      during the healing process has become a promising strategy. Here, a novel 
      "sandwich" cell culture platform with near-infrared (NIR) responsive dynamic 
      stiffness was fabricated to polarize bone marrow-derived macrophages (BMDMs) in 
      situ for revealing the relationship between the macrophage phenotype and 
      substrate stiffness dynamically. Under NIR irradiation, calcium ions (Ca(2+)) 
      diffused through the middle layer of the IR780-mixed phase change material (PCM) 
      due to the photothermal effect of IR780, resulting in an increase of hydrogel 
      stiffness in situ by the crosslinking of the upper layer of the hyaluronic 
      acid-sodium alginate hydrogel (MA-HA&SA). The up-regulation of inducible nitric 
      oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-alpha) was quantified by 
      immunostaining and enzyme-linked immune sorbent assay (ELISA), respectively, 
      indicating the transformation of macrophages from the anti-inflammatory to 
      pro-inflammatory phenotype under dynamic stiffness. The nuclear 
      Yes-associated-protein (YAP) ratio positively correlated with the shift of the 
      macrophage phenotype. The modulation of macrophage phenotypes by stiffness-rise 
      without the stimuli of cytokines offers an effective and noninvasive strategy to 
      manipulate immune reactions to achieve optimized healing or therapeutic outcomes.
FAU - Yuan, Peiqi
AU  - Yuan P
AD  - MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department 
      of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China. 
      liema@zju.edu.cn.
FAU - Luo, Yilun
AU  - Luo Y
FAU - Luo, Yu
AU  - Luo Y
FAU - Ma, Lie
AU  - Ma L
LA  - eng
PT  - Journal Article
DEP - 20210212
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
RN  - 0 (Hydrogels)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cell Culture Techniques
MH  - Hydrogels
MH  - *Macrophages
MH  - Phenotype
MH  - *Tumor Necrosis Factor-alpha
EDAT- 2021/02/13 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/02/12 08:40
PHST- 2021/02/13 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2021/02/12 08:40 [entrez]
AID - 10.1039/d0bm02194f [doi]
PST - ppublish
SO  - Biomater Sci. 2021 Apr 7;9(7):2553-2561. doi: 10.1039/d0bm02194f. Epub 2021 Feb 
      12.