PMID- 33576983
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210330
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2246
DP  - 2021
TI  - Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization.
PG  - 69-86
LID - 10.1007/978-1-0716-1115-9_5 [doi]
AB  - Traditionally, RNA and DNA probes are used in fluorescence in situ hybridization 
      (FISH) methods for microbial detection and characterization of communities' 
      structure and diversity. However, the recent introduction of nucleic acid 
      mimics (NAMs) has improved the robustness of the FISH methods in terms of 
      sensitivity and specificity. Several NAMs have been used, of which the most 
      relevant are peptide nucleic acid (PNA), locked nucleic acids (LNA), 2'-O-methyl 
      RNA (2'OMe), and phosphorothioates (PS). In this chapter, we describe a protocol 
      using PNA and LNA/2'OMe probes for microbial detection by FISH, pointing out the 
      differences between them. These protocols are easily adapted to different 
      microorganisms and different probe sequences.
FAU - Oliveira, Ricardo
AU  - Oliveira R
AD  - LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and 
      Energy, Department of Chemical Engineering, Faculty of Engineering, University of 
      Porto, Porto, Portugal.
AD  - INIAV - National Institute for Agrarian and Veterinarian Research, Rua dos 
      Lagidos, Lugar da Madalena, Vairao, Vila do Conde, Portugal.
FAU - Azevedo, Andreia S
AU  - Azevedo AS
AD  - LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and 
      Energy, Department of Chemical Engineering, Faculty of Engineering, University of 
      Porto, Porto, Portugal.
AD  - i3S - Instituto de Investigacao e Inovacao em Saude, Universidade do Porto, 
      Porto, Portugal.
AD  - IPATIMUP - Institute of Molecular Pathology and Immunology of the University of 
      Porto, University of Porto, Porto, Portugal.
AD  - CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal.
FAU - Mendes, Luzia
AU  - Mendes L
AD  - FMDUP - Faculty of Dental Medicine, University of Porto, Porto, Portugal. 
      lgoncalves@fmd.up.pt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Nucleic Acid Probes)
RN  - 0 (Nucleic Acids)
RN  - 0 (Oligonucleotides)
RN  - 0 (Peptide Nucleic Acids)
RN  - 0 (locked nucleic acid)
SB  - IM
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microbiota/genetics
MH  - Nucleic Acid Probes/genetics
MH  - Nucleic Acids/*genetics
MH  - Oligonucleotides/genetics
MH  - Peptide Nucleic Acids/genetics
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - 2'OMe
OT  - FISH
OT  - LNA
OT  - Microorganisms
OT  - Nucleic acid mimics
OT  - PNA
EDAT- 2021/02/13 06:00
MHDA- 2021/03/31 06:00
CRDT- 2021/02/12 12:12
PHST- 2021/02/12 12:12 [entrez]
PHST- 2021/02/13 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
AID - 10.1007/978-1-0716-1115-9_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2246:69-86. doi: 10.1007/978-1-0716-1115-9_5.