PMID- 33576986
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210330
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2246
DP  - 2021
TI  - Leaf-FISH: In Situ Hybridization Method for Visualizing Bacterial Taxa on Plant 
      Surfaces.
PG  - 111-128
LID - 10.1007/978-1-0716-1115-9_8 [doi]
AB  - High-resolution, spatial characterization of microbial communities is critical 
      for the accurate understanding of microbe-microbe and microbe-plant interactions 
      in leaf surfaces (phyllosphere). However, leaves are specially challenging 
      surfaces for imaging methods due to their high autofluorescence. In this chapter 
      we describe the Leaf-FISH method. Leaf-FISH is a fluorescence in situ 
      hybridization (FISH) method specially adapted to the requirements of plant 
      tissues. Leaf-FISH uses a combination of leaf pretreatments coupled with spectral 
      imaging confocal microscopy and image post-processing to visualize bacterial taxa 
      on a structural-informed context recreated from the residual background 
      autofluorescence of the tissues. Leaf-FISH is suitable for simultaneous 
      identification of multiple bacterial taxa using multiple taxon-specific 
      fluorescently labeled oligonucleotide probes (combinatorial labeling).
FAU - Peredo, Elena L
AU  - Peredo EL
AD  - Marine Biological Laboratory, The Ecosystems Center, Woods Hole, MA, USA. 
      elperedo@mbl.edu.
FAU - Simmons, Sheri
AU  - Simmons S
AD  - Marine Biological Laboratory, The Ecosystems Center, Woods Hole, MA, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Bacteria/genetics/*growth & development
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Confocal/methods
MH  - Oligonucleotide Probes/genetics
MH  - Plant Leaves/*microbiology
MH  - Plants/*microbiology
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - Combinatorial labeling
OT  - Fluorescence in situ hybridization
OT  - Leaf
OT  - Microenvironments
OT  - Phyllosphere
EDAT- 2021/02/13 06:00
MHDA- 2021/03/31 06:00
CRDT- 2021/02/12 12:12
PHST- 2021/02/12 12:12 [entrez]
PHST- 2021/02/13 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
AID - 10.1007/978-1-0716-1115-9_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2246:111-128. doi: 10.1007/978-1-0716-1115-9_8.