PMID- 33576988
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210330
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2246
DP  - 2021
TI  - Fluorescence In Situ Hybridization with Quantum Dot Labels in E. coli Cells.
PG  - 141-155
LID - 10.1007/978-1-0716-1115-9_10 [doi]
AB  - In this chapter we describe the use of fluorescent quantum dots (QDs) as labels 
      for microbial mRNA transcripts using fluorescence in situ hybridization (FISH). 
      Unlike organic dyes, which are the standard labels in modern FISH methods, QDs 
      provide fluorescence signals that are much brighter and resistant to 
      photobleaching, with an expanded spectral range for multiplexing. We describe the 
      preparation of QDs with compact sizes necessary for accurate labeling, their 
      application for analyzing lacZ transcripts in Escherichia coli cells using FISH, 
      and an assessment of signal stability. We further discuss differences between 
      methods for mammalian cells and bacteria, for which individual nucleic acids 
      cannot be discretely counted due to the small cell size and the optical 
      diffraction limit.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, 
      IL, USA.
AD  - Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, 
      Urbana, IL, USA.
FAU - Han, Zhiyuan
AU  - Han Z
AD  - Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, 
      IL, USA.
AD  - Department of Materials Science & Engineering, University of Illinois at 
      Urbana-Champaign, Urbana, IL, USA.
FAU - Sarkar, Suresh
AU  - Sarkar S
AD  - Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, 
      IL, USA.
AD  - Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, 
      Urbana, IL, USA.
FAU - Smith, Andrew M
AU  - Smith AM
AD  - Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, 
      IL, USA. smi@illinois.edu.
AD  - Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, 
      Urbana, IL, USA. smi@illinois.edu.
AD  - Department of Materials Science & Engineering, University of Illinois at 
      Urbana-Champaign, Urbana, IL, USA. smi@illinois.edu.
AD  - Carle Illinois College of Medicine, Urbana, IL, USA. smi@illinois.edu.
LA  - eng
GR  - R33 CA196458/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Escherichia coli/*metabolism
MH  - Fluorescence
MH  - Fluorescent Dyes/metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mammals/metabolism
MH  - Photobleaching
MH  - Quantum Dots/*metabolism
MH  - RNA, Messenger/metabolism
OTO - NOTNLM
OT  - Cytology
OT  - Imaging
OT  - Microscopy
OT  - Nanocrystal
OT  - Nanomedicine
OT  - Nanotechnology
OT  - Nucleic acid
OT  - QD-FISH
OT  - Single-cell
OT  - Single-molecule
EDAT- 2021/02/13 06:00
MHDA- 2021/03/31 06:00
CRDT- 2021/02/12 12:12
PHST- 2021/02/12 12:12 [entrez]
PHST- 2021/02/13 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
AID - 10.1007/978-1-0716-1115-9_10 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2246:141-155. doi: 10.1007/978-1-0716-1115-9_10.