PMID- 33576989
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210330
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2246
DP  - 2021
TI  - Monitoring Bacteriophage Infection on Bacterial Cells Using FISH.
PG  - 157-168
LID - 10.1007/978-1-0716-1115-9_11 [doi]
AB  - The possibility of visualizing bacteriophage-host interactions through 
      fluorescence in situ hybridization (FISH)-derived methods is gaining relevance in 
      the last few years. These methods allow the possibility of discriminating between 
      phage-infected and noninfected cells and the assessment of the different 
      infection stages at the single cell level. In opposition to bacterial cells, the 
      detection of phages is more challenging due to the low number of nucleic acid 
      copies. However, by using a conserved region of the phage genome that is highly 
      expressed during transcription, a FISH signal targeting phage DNA copies and mRNA 
      transcripts can be easily visible inside the bacterial host cells.In this book 
      chapter, we will cover both the design of locked nucleic acid (LNA) probes for 
      phages and a FISH method for the detection of phages inside bacterial cells.
FAU - Vilas-Boas, Diana
AU  - Vilas-Boas D
AD  - Laboratorio de Investigacao em Biofilmes Rosario Oliveira (LIBRO), Centre of 
      Biological Engineering (CEB), University of Minho, Braga, Portugal.
FAU - Melo, Luis D R
AU  - Melo LDR
AD  - Laboratorio de Investigacao em Biofilmes Rosario Oliveira (LIBRO), Centre of 
      Biological Engineering (CEB), University of Minho, Braga, Portugal. 
      lmelo@deb.uminho.pt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Oligonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 0 (locked nucleic acid)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Bacteria/*virology
MH  - Bacteriophages/*genetics
MH  - DNA/genetics
MH  - Host Microbial Interactions/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Oligonucleotides/genetics
MH  - RNA, Messenger/genetics
OTO - NOTNLM
OT  - Bacteriophage
OT  - Detection
OT  - Microscopy
OT  - Phage-host interactions
EDAT- 2021/02/13 06:00
MHDA- 2021/03/31 06:00
CRDT- 2021/02/12 12:13
PHST- 2021/02/12 12:13 [entrez]
PHST- 2021/02/13 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
AID - 10.1007/978-1-0716-1115-9_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2246:157-168. doi: 10.1007/978-1-0716-1115-9_11.