PMID- 33576998
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210330
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2246
DP  - 2021
TI  - Quality Control in Diagnostic Fluorescence In Situ Hybridization (FISH) in 
      Microbiology.
PG  - 301-316
LID - 10.1007/978-1-0716-1115-9_20 [doi]
AB  - This overview addresses fluorescence in situ hybridization (FISH) in a diagnostic 
      microbiology setting with its associated problems and pitfalls and how to control 
      them, but also the advantages and opportunities the method offers. This article 
      focuses mainly on diagnostic FISH assays on tissue sections and on techniques and 
      experiences in our laboratory. FISH in a routine diagnostic setting in 
      microbiology requires strict quality control measures to ensure consistent 
      high-quality and reliable assay results. Here, for the first time, we define 
      quality control requirements for microbiological diagnostic FISH applications and 
      discuss their impact and possible future developments of the FISH technique for 
      infection diagnostics. We focus on diagnosis of biofilm-associated infections 
      including infective endocarditis, oral biofilms, and device-associated infections 
      as well as infections due to fastidious or yet uncultured microorganisms like 
      Treponema spp., Tropheryma whipplei, Bartonella, Coxiella burnetii, or 
      Brachyspira.
FAU - Kikhney, Judith
AU  - Kikhney J
AD  - Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, 
      Charite-Universitatsmedizin Berlin, Berlin, Germany.
AD  - MoKi Analytics GmbH, Berlin, Germany.
FAU - Moter, Annette
AU  - Moter A
AD  - Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, 
      Charite-Universitatsmedizin Berlin, Berlin, Germany. annette.moter@charite.de.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Bacteria/*genetics
MH  - Biofilms/growth & development
MH  - Endocarditis, Bacterial/microbiology
MH  - Fluorescence
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microbiology
MH  - Quality Control
OTO - NOTNLM
OT  - Biofilm
OT  - Diagnostic FISH
OT  - Fluorescence in situ hybridization (FISH)
OT  - Microbiology
OT  - Molecular diagnosis
OT  - Pathogen identification
OT  - Quality control
EDAT- 2021/02/13 06:00
MHDA- 2021/03/31 06:00
CRDT- 2021/02/12 12:13
PHST- 2021/02/12 12:13 [entrez]
PHST- 2021/02/13 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
AID - 10.1007/978-1-0716-1115-9_20 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2246:301-316. doi: 10.1007/978-1-0716-1115-9_20.