PMID- 33577025
OWN - NLM
STAT- MEDLINE
DCOM- 20210629
LR  - 20210629
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 25
IP  - 2
DP  - 2021 Jan
TI  - Correlation between inflammatory cytokines and liver cancer stem cell markers in 
      DEN-induced liver cancer rats.
PG  - 710-721
LID - 24632 [pii]
LID - 10.26355/eurrev_202101_24632 [doi]
AB  - OBJECTIVE: To study the association of inflammatory factors and hepatocarcinoma 
      stem cells of induced liver cancer rats. MATERIALS AND METHODS: 30 SD male 
      healthy rats were selected. 10 rats were given water as normal control group. 10 
      rats only were implemented laparotomy as sham operation group. The remaining 10 
      rats were the liver cancer model group and treated with diethylnitrosamine (DEN) 
      to induce liver cancer. Real-time quantitative PCR was used to detect the related 
      inflammatory factors in HCC tissues, including interleukin-6 (IL-6), monocyte 
      chemoattractant protein-1 (MCP-1), transforming growth factor-beta1 (TGF-beta), human 
      interleukin-1alpha (IL-1alpha), human interleukin 1beta (IL-1beta) and levels of 
      hepatocarcinoma stem cells indicators CD90, CD133, Alpha-fetoprotein (AFP). 
      Correlation analysis was used to analyze the correlation between inflammatory 
      factors and hepatocarcinoma stem cells markers CD90 and CD133. RESULTS: The 
      expression levels of IL-6, MCP-1 and TGF-beta of HCC tissues in liver cancer model 
      group were significantly higher than those in the control group and the sham 
      operation group. The expression levels of CD90 and CD133 of tissues in the liver 
      cancer model group were significantly higher than those in the control group and 
      the sham operation group. The differences were statistically significant 
      (p<0.001). By inhibiting related inflammatory factors, the growth, migration and 
      invasion of liver cancer cells were significantly inhibited, and apoptosis was 
      promoted. Correlation analysis results showed that the expression changes of 
      IL-6, MCP-1 and TGF-beta were significantly positively correlated with CD90 
      up-regulation (p<0.05), while the expression changes of IL-6, MCP-1 and TGF-beta 
      were significantly positively correlated with CD133 up-regulation (p<0.05). 
      CONCLUSIONS: The inflammatory factors IL-6, MCP-1 and TGF-beta are closely related 
      to hepatocarcinoma stem cells, which play an important role in promoting the 
      occurrence and deterioration of liver cancer.
FAU - Liu, Z-H
AU  - Liu ZH
AD  - Department of Oncology, Medical School of Chinese PLA, Chinese PLA General 
      Hospital, Beijing, China. 755591296@qq.com.
FAU - Bai, M-J
AU  - Bai MJ
FAU - Li, J-Q
AU  - Li JQ
FAU - Yan, S-H
AU  - Yan SH
FAU - Ye, X-W
AU  - Ye XW
FAU - Bu, J-G
AU  - Bu JG
FAU - Chen, C-Y
AU  - Chen CY
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Il6 protein, rat)
RN  - 0 (Interleukin-6)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 3IQ78TTX1A (Diethylnitrosamine)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Apoptosis
MH  - Biomarkers, Tumor/*genetics/metabolism
MH  - Cell Proliferation
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Diethylnitrosamine/administration & dosage
MH  - Disease Models, Animal
MH  - Interleukin-6/*genetics/metabolism
MH  - Liver Neoplasms/chemically induced/*metabolism/pathology
MH  - Male
MH  - Neoplastic Stem Cells/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor beta1/*genetics/metabolism
EDAT- 2021/02/13 06:00
MHDA- 2021/06/30 06:00
CRDT- 2021/02/12 12:13
PHST- 2021/02/12 12:13 [entrez]
PHST- 2021/02/13 06:00 [pubmed]
PHST- 2021/06/30 06:00 [medline]
AID - 24632 [pii]
AID - 10.26355/eurrev_202101_24632 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2021 Jan;25(2):710-721. doi: 
      10.26355/eurrev_202101_24632.