PMID- 33581126
OWN - NLM
STAT- MEDLINE
DCOM- 20210317
LR  - 20211204
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 271
DP  - 2021 Apr 15
TI  - Enterovirus 71 induces autophagy in mice via mTOR inhibition and ERK pathway 
      activation.
PG  - 119188
LID - S0024-3205(21)00173-9 [pii]
LID - 10.1016/j.lfs.2021.119188 [doi]
AB  - AIMS: Enterovirus 71 (EV71) is one of the main viruses that cause hand-foot-mouth 
      disease; however, its pathogenic mechanism remains unclear. This study 
      characterized the relationship between EV71 infection and autophagy in vivo and 
      explored the molecular mechanism underlying EV71-induced autophagy. MATERIALS AND 
      METHODS: A mouse model of EV71 infection was prepared by intraperitoneally 
      injecting one-day-old BALB/c suckling mice with 30 muL/g of EV71 virus stock 
      solution for 3 days. The behavior, fur condition, weight, and mice mortality were 
      monitored, and disease scores were calculated. The pathological damage to the 
      brain, lung, and muscle tissues after the viral infection was assessed by 
      hematoxylin and eosin staining. Western blot and immunofluorescence analyses were 
      used to detect the expression levels of viral protein 1, Beclin-1, 
      microtubule-associated protein light chain 3B, mammalian target of rapamycin 
      (mTOR), phosphorylated (p)-mTOR, extracellular signal-regulated protein kinase 
      (ERK) 1/2, and p-ERK. KEY FINDINGS: EV71 infection can trigger autophagy in the 
      brains, lungs, and muscles of infected mice. The autophagy response triggered by 
      EV71 is achieved by the simultaneous mTOR inhibition and the ERK pathway 
      activation. Blocking the mTOR pathway may aggravate autophagy, whereas ERK 
      inhibition alleviates autophagy but cannot completely prevent it. SIGNIFICANCE: 
      EV71 infection can induce autophagy in mice, involving mTOR and ERK signaling 
      pathways. These two signaling pathways are independent and do not interfere with 
      each other.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Wang, Baixin
AU  - Wang B
AD  - School of Basic Medicine, Jiamusi University, Jiamusi 154007, China.
FAU - Zhu, Yuanzhi
AU  - Zhu Y
AD  - School of Basic Medicine, Jiamusi University, Jiamusi 154007, China.
FAU - Liu, Lei
AU  - Liu L
AD  - School of Basic Medicine, Jiamusi University, Jiamusi 154007, China.
FAU - Wang, Binshan
AU  - Wang B
AD  - School of Basic Medicine, Jiamusi University, Jiamusi 154007, China.
FAU - Chen, Mei
AU  - Chen M
AD  - School of Basic Medicine, Jiamusi University, Jiamusi 154007, China.
FAU - Wang, Jingtao
AU  - Wang J
AD  - School of Basic Medicine, Jiamusi University, Jiamusi 154007, China.
FAU - Yang, Limin
AU  - Yang L
AD  - School of Medicine, Dalian University, Dalian 116622, China. Electronic address: 
      yanglimin@dlu.edu.cn.
FAU - Liu, JiGuang
AU  - Liu J
AD  - School of Basic Medicine, Jiamusi University, Jiamusi 154007, China. Electronic 
      address: 13694668555@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210211
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Autophagy/*physiology
MH  - Cell Line, Tumor
MH  - Enterovirus A, Human/*metabolism
MH  - Enterovirus Infections/*metabolism/pathology
MH  - Enzyme Activation/physiology
MH  - Female
MH  - Humans
MH  - MAP Kinase Signaling System/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - TOR Serine-Threonine Kinases/*antagonists & inhibitors/*metabolism
OTO - NOTNLM
OT  - Autophagy
OT  - Enterovirus 71
OT  - Extracellular signal-regulated kinase
OT  - Hand-foot-mouth disease
OT  - Mammalian target of rapamycin
EDAT- 2021/02/14 06:00
MHDA- 2021/03/18 06:00
CRDT- 2021/02/13 20:09
PHST- 2020/10/25 00:00 [received]
PHST- 2021/02/01 00:00 [revised]
PHST- 2021/02/02 00:00 [accepted]
PHST- 2021/02/14 06:00 [pubmed]
PHST- 2021/03/18 06:00 [medline]
PHST- 2021/02/13 20:09 [entrez]
AID - S0024-3205(21)00173-9 [pii]
AID - 10.1016/j.lfs.2021.119188 [doi]
PST - ppublish
SO  - Life Sci. 2021 Apr 15;271:119188. doi: 10.1016/j.lfs.2021.119188. Epub 2021 Feb 
      11.